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The Hydroalcoholic Extract of Matricaria chamomilla Suppresses Migration and Invasion of Human Breast Cancer MDA-MB-468 and MCF-7 Cell Lines

BACKGROUND: Matricaria chamomilla is an aromatic plant with antioxidant, anticancer, and anti-inflammatory properties. However, the inhibitory role of M. chamomilla on migration and invasion of human breast cancer cells remains unclear. OBJECTIVE: This study investigated the methods to evaluate thes...

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Autores principales: Nikseresht, Mohsen, Kamali, Ali Mohammad, Rahimi, Hamid Reza, Delaviz, Hamdollah, Toori, Mehdi Akbartabar, Kashani, Iraj Ragerdi, Mahmoudi, Reza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5330110/
https://www.ncbi.nlm.nih.gov/pubmed/28250660
http://dx.doi.org/10.4103/0974-8490.199778
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author Nikseresht, Mohsen
Kamali, Ali Mohammad
Rahimi, Hamid Reza
Delaviz, Hamdollah
Toori, Mehdi Akbartabar
Kashani, Iraj Ragerdi
Mahmoudi, Reza
author_facet Nikseresht, Mohsen
Kamali, Ali Mohammad
Rahimi, Hamid Reza
Delaviz, Hamdollah
Toori, Mehdi Akbartabar
Kashani, Iraj Ragerdi
Mahmoudi, Reza
author_sort Nikseresht, Mohsen
collection PubMed
description BACKGROUND: Matricaria chamomilla is an aromatic plant with antioxidant, anticancer, and anti-inflammatory properties. However, the inhibitory role of M. chamomilla on migration and invasion of human breast cancer cells remains unclear. OBJECTIVE: This study investigated the methods to evaluate these anticancer mechanisms of M. chamomilla on human breast cancer MCF-7 and MDA-MB-468 cell lines. MATERIALS AND METHODS: The cells were treated with hydroalcoholic extract of M. chamomilla at different concentrations (50–1300 μg/mL) for 24, 48, and 72 h in a culture medium containing 10% fetal bovine serum. This study quantified the 50% growth inhibition concentrations (IC(50)) by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay; apoptosis and necrosis through Hoechst 33342/propidium iodide staining; cell proliferation and clone formation by clonogenic assay as well as cellular migration, invasion, and attachment. After 24, 48, and 72 h of treatment, the IC(50)levels were 992 ± 2.3 μg/mL, 893 ± 5.4 μg/mL, and 785 ± 4.8 μg/mL against MDA-MB-468, respectively, and 1288 ± 5.6 μg/mL, 926 ± 2.5 μg/mL, and 921 ± 3.5 μg/mL, against MCF-7, respectively. Furthermore, increasing the extract concentrations induced cellular apoptosis and necrosis and decreased cellular invasion or migration through 8 μm pores, colonization and attachment in a dose-dependent manner. RESULTS: It indicated time- and dose-dependent anti-invasive and antimigrative or proliferative and antitoxic effects of hydroalcoholic extract of aerial parts of chamomile on breast cancer cells. CONCLUSION: This study demonstrated an effective plant in preventing or treating breast cancer. SUMMARY: Antioxidant compounds in Matricaria chamomilla have anticancer effects. Hydroalcoholic extract of M. chamomilla controls cellular proliferation and apoptosis induction. Hoechst 33342/propidium iodide staining suggested that the extract induces apoptosis more than necrosis. Hydroalcoholic extract of M. chamomilla prevents colonization and cellular migration of human breast cancer MDA-MB-468 and MCF-7 cell lines in a time- and dose-dependent manner. M. chamomilla has low cytotoxic effects on natural cells. [Image: see text] Abbreviations Used: IARC: International Agency for Research on Cancer; WHO: World Health Organization; FBS: Fetal bovine serum; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; DMSO: Dimethyl sulfoxide; PI: Propidium iodide; LN: Live cells with normal nucleus; LA: Live cells with apoptized nucleus; DN: Dead cells with normal nucleus; DA: Dead cells with apoptized nucleus; BSA: Bovine serum albumin; ANOVA: Analysis of variance; IC(50): 50% growth inhibition concentration; GSE: Grape seed extract
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spelling pubmed-53301102017-03-01 The Hydroalcoholic Extract of Matricaria chamomilla Suppresses Migration and Invasion of Human Breast Cancer MDA-MB-468 and MCF-7 Cell Lines Nikseresht, Mohsen Kamali, Ali Mohammad Rahimi, Hamid Reza Delaviz, Hamdollah Toori, Mehdi Akbartabar Kashani, Iraj Ragerdi Mahmoudi, Reza Pharmacognosy Res Original Article BACKGROUND: Matricaria chamomilla is an aromatic plant with antioxidant, anticancer, and anti-inflammatory properties. However, the inhibitory role of M. chamomilla on migration and invasion of human breast cancer cells remains unclear. OBJECTIVE: This study investigated the methods to evaluate these anticancer mechanisms of M. chamomilla on human breast cancer MCF-7 and MDA-MB-468 cell lines. MATERIALS AND METHODS: The cells were treated with hydroalcoholic extract of M. chamomilla at different concentrations (50–1300 μg/mL) for 24, 48, and 72 h in a culture medium containing 10% fetal bovine serum. This study quantified the 50% growth inhibition concentrations (IC(50)) by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay; apoptosis and necrosis through Hoechst 33342/propidium iodide staining; cell proliferation and clone formation by clonogenic assay as well as cellular migration, invasion, and attachment. After 24, 48, and 72 h of treatment, the IC(50)levels were 992 ± 2.3 μg/mL, 893 ± 5.4 μg/mL, and 785 ± 4.8 μg/mL against MDA-MB-468, respectively, and 1288 ± 5.6 μg/mL, 926 ± 2.5 μg/mL, and 921 ± 3.5 μg/mL, against MCF-7, respectively. Furthermore, increasing the extract concentrations induced cellular apoptosis and necrosis and decreased cellular invasion or migration through 8 μm pores, colonization and attachment in a dose-dependent manner. RESULTS: It indicated time- and dose-dependent anti-invasive and antimigrative or proliferative and antitoxic effects of hydroalcoholic extract of aerial parts of chamomile on breast cancer cells. CONCLUSION: This study demonstrated an effective plant in preventing or treating breast cancer. SUMMARY: Antioxidant compounds in Matricaria chamomilla have anticancer effects. Hydroalcoholic extract of M. chamomilla controls cellular proliferation and apoptosis induction. Hoechst 33342/propidium iodide staining suggested that the extract induces apoptosis more than necrosis. Hydroalcoholic extract of M. chamomilla prevents colonization and cellular migration of human breast cancer MDA-MB-468 and MCF-7 cell lines in a time- and dose-dependent manner. M. chamomilla has low cytotoxic effects on natural cells. [Image: see text] Abbreviations Used: IARC: International Agency for Research on Cancer; WHO: World Health Organization; FBS: Fetal bovine serum; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; DMSO: Dimethyl sulfoxide; PI: Propidium iodide; LN: Live cells with normal nucleus; LA: Live cells with apoptized nucleus; DN: Dead cells with normal nucleus; DA: Dead cells with apoptized nucleus; BSA: Bovine serum albumin; ANOVA: Analysis of variance; IC(50): 50% growth inhibition concentration; GSE: Grape seed extract Medknow Publications & Media Pvt Ltd 2017 /pmc/articles/PMC5330110/ /pubmed/28250660 http://dx.doi.org/10.4103/0974-8490.199778 Text en Copyright: © 2017 Pharmacognosy Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Nikseresht, Mohsen
Kamali, Ali Mohammad
Rahimi, Hamid Reza
Delaviz, Hamdollah
Toori, Mehdi Akbartabar
Kashani, Iraj Ragerdi
Mahmoudi, Reza
The Hydroalcoholic Extract of Matricaria chamomilla Suppresses Migration and Invasion of Human Breast Cancer MDA-MB-468 and MCF-7 Cell Lines
title The Hydroalcoholic Extract of Matricaria chamomilla Suppresses Migration and Invasion of Human Breast Cancer MDA-MB-468 and MCF-7 Cell Lines
title_full The Hydroalcoholic Extract of Matricaria chamomilla Suppresses Migration and Invasion of Human Breast Cancer MDA-MB-468 and MCF-7 Cell Lines
title_fullStr The Hydroalcoholic Extract of Matricaria chamomilla Suppresses Migration and Invasion of Human Breast Cancer MDA-MB-468 and MCF-7 Cell Lines
title_full_unstemmed The Hydroalcoholic Extract of Matricaria chamomilla Suppresses Migration and Invasion of Human Breast Cancer MDA-MB-468 and MCF-7 Cell Lines
title_short The Hydroalcoholic Extract of Matricaria chamomilla Suppresses Migration and Invasion of Human Breast Cancer MDA-MB-468 and MCF-7 Cell Lines
title_sort hydroalcoholic extract of matricaria chamomilla suppresses migration and invasion of human breast cancer mda-mb-468 and mcf-7 cell lines
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5330110/
https://www.ncbi.nlm.nih.gov/pubmed/28250660
http://dx.doi.org/10.4103/0974-8490.199778
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