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Fine selection of up-regulated genes during ovulation by in vivo induction of oocyte maturation and ovulation in zebrafish

BACKGROUND: Two essential processes, oocyte maturation and ovulation, are independently induced, but proceed cooperatively as the final step in oogenesis before oocytes become fertilizable. Although these two processes are induced by the same maturation-inducing steroid, 17α, 20β-dihydroxy-4-pregnen...

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Autores principales: Klangnurak, Wanlada, Tokumoto, Toshinobu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5330128/
https://www.ncbi.nlm.nih.gov/pubmed/28265462
http://dx.doi.org/10.1186/s40851-017-0065-8
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author Klangnurak, Wanlada
Tokumoto, Toshinobu
author_facet Klangnurak, Wanlada
Tokumoto, Toshinobu
author_sort Klangnurak, Wanlada
collection PubMed
description BACKGROUND: Two essential processes, oocyte maturation and ovulation, are independently induced, but proceed cooperatively as the final step in oogenesis before oocytes become fertilizable. Although these two processes are induced by the same maturation-inducing steroid, 17α, 20β-dihydroxy-4-pregnen-3-one (17, 20β-DHP), in zebrafish, it has been suggested that the receptor, and thus the signal transduction pathway is different for each process. Although much progress has been made in understanding the molecular mechanisms underlying the induction of oocyte maturation, the mechanisms for inducing ovulation remain under investigation. In the present study, in vivo induction techniques that permit the induction of oocyte maturation and ovulation in living zebrafish (in vivo assays) were used to select highly up-regulated genes (genes associated with ovulation). Using an in vivo assay, ovarian tissues that induced only oocyte maturation could be obtained. This made it possible for the first time to distinguish maturation-inducing genes from ovulation-inducing genes. Using a genome-wide microarray of zebrafish sequences, the gene expression levels were compared among an ethanol (EtOH)-treated group (non-activated group), a diethylstilbestrol (DES)- or testosterone (Tes)-treated group (maturation-induced group), and a 17, 20β-DHP-treated group (maturation- and ovulation-induced group). Ovulation-specific up-regulated genes were selected. The mRNA expression levels of the selected genes were measured by quantitative polymerase chain reaction (qPCR). RESULTS: Among 34 genes identified, three that showed ovulation-specific increases were selected as candidates potentially associated with ovulation. The ovulation-specific up-regulation of three candidates, slc37a4a, zgc:65811 and zgc:92184 was confirmed by qPCR. CONCLUSION: Our in vivo assay provides a new approach to precisely select genes associated with ovulation.
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spelling pubmed-53301282017-03-06 Fine selection of up-regulated genes during ovulation by in vivo induction of oocyte maturation and ovulation in zebrafish Klangnurak, Wanlada Tokumoto, Toshinobu Zoological Lett Research Article BACKGROUND: Two essential processes, oocyte maturation and ovulation, are independently induced, but proceed cooperatively as the final step in oogenesis before oocytes become fertilizable. Although these two processes are induced by the same maturation-inducing steroid, 17α, 20β-dihydroxy-4-pregnen-3-one (17, 20β-DHP), in zebrafish, it has been suggested that the receptor, and thus the signal transduction pathway is different for each process. Although much progress has been made in understanding the molecular mechanisms underlying the induction of oocyte maturation, the mechanisms for inducing ovulation remain under investigation. In the present study, in vivo induction techniques that permit the induction of oocyte maturation and ovulation in living zebrafish (in vivo assays) were used to select highly up-regulated genes (genes associated with ovulation). Using an in vivo assay, ovarian tissues that induced only oocyte maturation could be obtained. This made it possible for the first time to distinguish maturation-inducing genes from ovulation-inducing genes. Using a genome-wide microarray of zebrafish sequences, the gene expression levels were compared among an ethanol (EtOH)-treated group (non-activated group), a diethylstilbestrol (DES)- or testosterone (Tes)-treated group (maturation-induced group), and a 17, 20β-DHP-treated group (maturation- and ovulation-induced group). Ovulation-specific up-regulated genes were selected. The mRNA expression levels of the selected genes were measured by quantitative polymerase chain reaction (qPCR). RESULTS: Among 34 genes identified, three that showed ovulation-specific increases were selected as candidates potentially associated with ovulation. The ovulation-specific up-regulation of three candidates, slc37a4a, zgc:65811 and zgc:92184 was confirmed by qPCR. CONCLUSION: Our in vivo assay provides a new approach to precisely select genes associated with ovulation. BioMed Central 2017-02-28 /pmc/articles/PMC5330128/ /pubmed/28265462 http://dx.doi.org/10.1186/s40851-017-0065-8 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Klangnurak, Wanlada
Tokumoto, Toshinobu
Fine selection of up-regulated genes during ovulation by in vivo induction of oocyte maturation and ovulation in zebrafish
title Fine selection of up-regulated genes during ovulation by in vivo induction of oocyte maturation and ovulation in zebrafish
title_full Fine selection of up-regulated genes during ovulation by in vivo induction of oocyte maturation and ovulation in zebrafish
title_fullStr Fine selection of up-regulated genes during ovulation by in vivo induction of oocyte maturation and ovulation in zebrafish
title_full_unstemmed Fine selection of up-regulated genes during ovulation by in vivo induction of oocyte maturation and ovulation in zebrafish
title_short Fine selection of up-regulated genes during ovulation by in vivo induction of oocyte maturation and ovulation in zebrafish
title_sort fine selection of up-regulated genes during ovulation by in vivo induction of oocyte maturation and ovulation in zebrafish
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5330128/
https://www.ncbi.nlm.nih.gov/pubmed/28265462
http://dx.doi.org/10.1186/s40851-017-0065-8
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