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Uranium XAFS analysis of kidney from rats exposed to uranium
The kidney is the critical target of uranium exposure because uranium accumulates in the proximal tubules and causes tubular damage, but the chemical nature of uranium in kidney, such as its chemical status in the toxic target site, is poorly understood. Micro-X-ray absorption fine-structure (µXAFS)...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5330292/ https://www.ncbi.nlm.nih.gov/pubmed/28244440 http://dx.doi.org/10.1107/S1600577517001850 |
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author | Kitahara, Keisuke Numako, Chiya Terada, Yasuko Nitta, Kiyohumi Shimada, Yoshiya Homma-Takeda, Shino |
author_facet | Kitahara, Keisuke Numako, Chiya Terada, Yasuko Nitta, Kiyohumi Shimada, Yoshiya Homma-Takeda, Shino |
author_sort | Kitahara, Keisuke |
collection | PubMed |
description | The kidney is the critical target of uranium exposure because uranium accumulates in the proximal tubules and causes tubular damage, but the chemical nature of uranium in kidney, such as its chemical status in the toxic target site, is poorly understood. Micro-X-ray absorption fine-structure (µXAFS) analysis was used to examine renal thin sections of rats exposed to uranyl acetate. The U L (III)-edge X-ray absorption near-edge structure spectra of bulk renal specimens obtained at various toxicological phases were similar to that of uranyl acetate: their edge position did not shift compared with that of uranyl acetate (17.175 keV) although the peak widths for some kidney specimens were slightly narrowed. µXAFS measurements of spots of concentrated uranium in the micro-regions of the proximal tubules showed that the edge jump slightly shifted to lower energy. The results suggest that most uranium accumulated in kidney was uranium (VI) but a portion might have been biotransformed in rats exposed to uranyl acetate. |
format | Online Article Text |
id | pubmed-5330292 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-53302922017-03-01 Uranium XAFS analysis of kidney from rats exposed to uranium Kitahara, Keisuke Numako, Chiya Terada, Yasuko Nitta, Kiyohumi Shimada, Yoshiya Homma-Takeda, Shino J Synchrotron Radiat Research Papers The kidney is the critical target of uranium exposure because uranium accumulates in the proximal tubules and causes tubular damage, but the chemical nature of uranium in kidney, such as its chemical status in the toxic target site, is poorly understood. Micro-X-ray absorption fine-structure (µXAFS) analysis was used to examine renal thin sections of rats exposed to uranyl acetate. The U L (III)-edge X-ray absorption near-edge structure spectra of bulk renal specimens obtained at various toxicological phases were similar to that of uranyl acetate: their edge position did not shift compared with that of uranyl acetate (17.175 keV) although the peak widths for some kidney specimens were slightly narrowed. µXAFS measurements of spots of concentrated uranium in the micro-regions of the proximal tubules showed that the edge jump slightly shifted to lower energy. The results suggest that most uranium accumulated in kidney was uranium (VI) but a portion might have been biotransformed in rats exposed to uranyl acetate. International Union of Crystallography 2017-02-20 /pmc/articles/PMC5330292/ /pubmed/28244440 http://dx.doi.org/10.1107/S1600577517001850 Text en © Keisuke Kitahara et al. 2017 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/2.0/uk/ |
spellingShingle | Research Papers Kitahara, Keisuke Numako, Chiya Terada, Yasuko Nitta, Kiyohumi Shimada, Yoshiya Homma-Takeda, Shino Uranium XAFS analysis of kidney from rats exposed to uranium |
title | Uranium XAFS analysis of kidney from rats exposed to uranium |
title_full | Uranium XAFS analysis of kidney from rats exposed to uranium |
title_fullStr | Uranium XAFS analysis of kidney from rats exposed to uranium |
title_full_unstemmed | Uranium XAFS analysis of kidney from rats exposed to uranium |
title_short | Uranium XAFS analysis of kidney from rats exposed to uranium |
title_sort | uranium xafs analysis of kidney from rats exposed to uranium |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5330292/ https://www.ncbi.nlm.nih.gov/pubmed/28244440 http://dx.doi.org/10.1107/S1600577517001850 |
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