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Environmental DNA reflects spatial and temporal jellyfish distribution
Recent development of environmental DNA (eDNA) analysis allows us to survey underwater macro-organisms easily and cost effectively; however, there have been no reports on eDNA detection or quantification for jellyfish. Here we present the first report on an eDNA analysis of marine jellyfish using Ja...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5330514/ https://www.ncbi.nlm.nih.gov/pubmed/28245277 http://dx.doi.org/10.1371/journal.pone.0173073 |
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author | Minamoto, Toshifumi Fukuda, Miho Katsuhara, Koki R. Fujiwara, Ayaka Hidaka, Shunsuke Yamamoto, Satoshi Takahashi, Kohji Masuda, Reiji |
author_facet | Minamoto, Toshifumi Fukuda, Miho Katsuhara, Koki R. Fujiwara, Ayaka Hidaka, Shunsuke Yamamoto, Satoshi Takahashi, Kohji Masuda, Reiji |
author_sort | Minamoto, Toshifumi |
collection | PubMed |
description | Recent development of environmental DNA (eDNA) analysis allows us to survey underwater macro-organisms easily and cost effectively; however, there have been no reports on eDNA detection or quantification for jellyfish. Here we present the first report on an eDNA analysis of marine jellyfish using Japanese sea nettle (Chrysaora pacifica) as a model species by combining a tank experiment with spatial and temporal distribution surveys. We performed a tank experiment monitoring eDNA concentrations over a range of time intervals after the introduction of jellyfish, and quantified the eDNA concentrations by quantitative real-time PCR. The eDNA concentrations peaked twice, at 1 and 8 h after the beginning of the experiment, and became stable within 48 h. The estimated release rates of the eDNA in jellyfish were higher than the rates previously reported in fishes. A spatial survey was conducted in June 2014 in Maizuru Bay, Kyoto, in which eDNA was collected from surface water and sea floor water samples at 47 sites while jellyfish near surface water were counted on board by eye. The distribution of eDNA in the bay corresponded with the distribution of jellyfish inferred by visual observation, and the eDNA concentration in the bay was ~13 times higher on the sea floor than on the surface. The temporal survey was conducted from March to November 2014, in which jellyfish were counted by eye every morning while eDNA was collected from surface and sea floor water at three sampling points along a pier once a month. The temporal fluctuation pattern of the eDNA concentrations and the numbers of observed individuals were well correlated. We conclude that an eDNA approach is applicable for jellyfish species in the ocean. |
format | Online Article Text |
id | pubmed-5330514 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-53305142017-03-09 Environmental DNA reflects spatial and temporal jellyfish distribution Minamoto, Toshifumi Fukuda, Miho Katsuhara, Koki R. Fujiwara, Ayaka Hidaka, Shunsuke Yamamoto, Satoshi Takahashi, Kohji Masuda, Reiji PLoS One Research Article Recent development of environmental DNA (eDNA) analysis allows us to survey underwater macro-organisms easily and cost effectively; however, there have been no reports on eDNA detection or quantification for jellyfish. Here we present the first report on an eDNA analysis of marine jellyfish using Japanese sea nettle (Chrysaora pacifica) as a model species by combining a tank experiment with spatial and temporal distribution surveys. We performed a tank experiment monitoring eDNA concentrations over a range of time intervals after the introduction of jellyfish, and quantified the eDNA concentrations by quantitative real-time PCR. The eDNA concentrations peaked twice, at 1 and 8 h after the beginning of the experiment, and became stable within 48 h. The estimated release rates of the eDNA in jellyfish were higher than the rates previously reported in fishes. A spatial survey was conducted in June 2014 in Maizuru Bay, Kyoto, in which eDNA was collected from surface water and sea floor water samples at 47 sites while jellyfish near surface water were counted on board by eye. The distribution of eDNA in the bay corresponded with the distribution of jellyfish inferred by visual observation, and the eDNA concentration in the bay was ~13 times higher on the sea floor than on the surface. The temporal survey was conducted from March to November 2014, in which jellyfish were counted by eye every morning while eDNA was collected from surface and sea floor water at three sampling points along a pier once a month. The temporal fluctuation pattern of the eDNA concentrations and the numbers of observed individuals were well correlated. We conclude that an eDNA approach is applicable for jellyfish species in the ocean. Public Library of Science 2017-02-28 /pmc/articles/PMC5330514/ /pubmed/28245277 http://dx.doi.org/10.1371/journal.pone.0173073 Text en © 2017 Minamoto et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Minamoto, Toshifumi Fukuda, Miho Katsuhara, Koki R. Fujiwara, Ayaka Hidaka, Shunsuke Yamamoto, Satoshi Takahashi, Kohji Masuda, Reiji Environmental DNA reflects spatial and temporal jellyfish distribution |
title | Environmental DNA reflects spatial and temporal jellyfish distribution |
title_full | Environmental DNA reflects spatial and temporal jellyfish distribution |
title_fullStr | Environmental DNA reflects spatial and temporal jellyfish distribution |
title_full_unstemmed | Environmental DNA reflects spatial and temporal jellyfish distribution |
title_short | Environmental DNA reflects spatial and temporal jellyfish distribution |
title_sort | environmental dna reflects spatial and temporal jellyfish distribution |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5330514/ https://www.ncbi.nlm.nih.gov/pubmed/28245277 http://dx.doi.org/10.1371/journal.pone.0173073 |
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