Procedures for cryogenic X-ray ptychographic imaging of biological samples

Biological sample-preparation procedures have been developed for imaging human chromosomes under cryogenic conditions. A new experimental setup, developed for imaging frozen samples using beamline I13 at Diamond Light Source, is described. This manuscript describes the equipment and experimental pro...

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Detalles Bibliográficos
Autores principales: Yusuf, M., Zhang, F., Chen, B., Bhartiya, A., Cunnea, K., Wagner, U., Cacho-Nerin, F., Schwenke, J., Robinson, I. K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2017
Materias:
Research Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5330525/
https://www.ncbi.nlm.nih.gov/pubmed/28250953
http://dx.doi.org/10.1107/S2052252516020029
Descripción
Sumario:Biological sample-preparation procedures have been developed for imaging human chromosomes under cryogenic conditions. A new experimental setup, developed for imaging frozen samples using beamline I13 at Diamond Light Source, is described. This manuscript describes the equipment and experimental procedures as well as the authors’ first ptychographic reconstructions using X-rays.

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