Procedures for cryogenic X-ray ptychographic imaging of biological samples

Biological sample-preparation procedures have been developed for imaging human chromosomes under cryogenic conditions. A new experimental setup, developed for imaging frozen samples using beamline I13 at Diamond Light Source, is described. This manuscript describes the equipment and experimental pro...

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Detalles Bibliográficos
Autores principales: Yusuf, M., Zhang, F., Chen, B., Bhartiya, A., Cunnea, K., Wagner, U., Cacho-Nerin, F., Schwenke, J., Robinson, I. K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2017
Materias:
Research Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5330525/
https://www.ncbi.nlm.nih.gov/pubmed/28250953
http://dx.doi.org/10.1107/S2052252516020029
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author Yusuf, M.
Zhang, F.
Chen, B.
Bhartiya, A.
Cunnea, K.
Wagner, U.
Cacho-Nerin, F.
Schwenke, J.
Robinson, I. K.
author_facet Yusuf, M.
Zhang, F.
Chen, B.
Bhartiya, A.
Cunnea, K.
Wagner, U.
Cacho-Nerin, F.
Schwenke, J.
Robinson, I. K.
author_sort Yusuf, M.
collection PubMed
description Biological sample-preparation procedures have been developed for imaging human chromosomes under cryogenic conditions. A new experimental setup, developed for imaging frozen samples using beamline I13 at Diamond Light Source, is described. This manuscript describes the equipment and experimental procedures as well as the authors’ first ptychographic reconstructions using X-rays.
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spelling pubmed-53305252017-03-01 Procedures for cryogenic X-ray ptychographic imaging of biological samples Yusuf, M. Zhang, F. Chen, B. Bhartiya, A. Cunnea, K. Wagner, U. Cacho-Nerin, F. Schwenke, J. Robinson, I. K. IUCrJ Research Papers Biological sample-preparation procedures have been developed for imaging human chromosomes under cryogenic conditions. A new experimental setup, developed for imaging frozen samples using beamline I13 at Diamond Light Source, is described. This manuscript describes the equipment and experimental procedures as well as the authors’ first ptychographic reconstructions using X-rays. International Union of Crystallography 2017-01-12 /pmc/articles/PMC5330525/ /pubmed/28250953 http://dx.doi.org/10.1107/S2052252516020029 Text en © M. Yusuf et al. 2017 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/2.0/uk/
spellingShingle Research Papers
Yusuf, M.
Zhang, F.
Chen, B.
Bhartiya, A.
Cunnea, K.
Wagner, U.
Cacho-Nerin, F.
Schwenke, J.
Robinson, I. K.
Procedures for cryogenic X-ray ptychographic imaging of biological samples
title Procedures for cryogenic X-ray ptychographic imaging of biological samples
title_full Procedures for cryogenic X-ray ptychographic imaging of biological samples
title_fullStr Procedures for cryogenic X-ray ptychographic imaging of biological samples
title_full_unstemmed Procedures for cryogenic X-ray ptychographic imaging of biological samples
title_short Procedures for cryogenic X-ray ptychographic imaging of biological samples
title_sort procedures for cryogenic x-ray ptychographic imaging of biological samples
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5330525/
https://www.ncbi.nlm.nih.gov/pubmed/28250953
http://dx.doi.org/10.1107/S2052252516020029
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