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The Cardiovascular and Neurotoxic Effects of the Venoms of Six Bony and Cartilaginous Fish Species
Fish venoms are often poorly studied, in part due to the difficulty in obtaining, extracting, and storing them. In this study, we characterize the cardiovascular and neurotoxic effects of the venoms from the following six species of fish: the cartilaginous stingrays Neotrygon kuhlii and Himantura to...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5331446/ https://www.ncbi.nlm.nih.gov/pubmed/28212333 http://dx.doi.org/10.3390/toxins9020067 |
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author | Han, Han Baumann, Kate Casewell, Nicholas R. Ali, Syed A. Dobson, James Koludarov, Ivan Debono, Jordan Cutmore, Scott C. Rajapakse, Niwanthi W. Jackson, Timothy N. W. Jones, Rob Hodgson, Wayne C. Fry, Bryan G. Kuruppu, Sanjaya |
author_facet | Han, Han Baumann, Kate Casewell, Nicholas R. Ali, Syed A. Dobson, James Koludarov, Ivan Debono, Jordan Cutmore, Scott C. Rajapakse, Niwanthi W. Jackson, Timothy N. W. Jones, Rob Hodgson, Wayne C. Fry, Bryan G. Kuruppu, Sanjaya |
author_sort | Han, Han |
collection | PubMed |
description | Fish venoms are often poorly studied, in part due to the difficulty in obtaining, extracting, and storing them. In this study, we characterize the cardiovascular and neurotoxic effects of the venoms from the following six species of fish: the cartilaginous stingrays Neotrygon kuhlii and Himantura toshi, and the bony fish Platycephalus fucus, Girella tricuspidata, Mugil cephalus, and Dentex tumifrons. All venoms (10–100 µg/kg, i.v.), except G. tricuspidata and P. fuscus, induced a biphasic response on mean arterial pressure (MAP) in the anesthetised rat. P. fucus venom exhibited a hypotensive response, while venom from G. tricuspidata displayed a single depressor response. All venoms induced cardiovascular collapse at 200 µg/kg, i.v. The in vitro neurotoxic effects of venom were examined using the chick biventer cervicis nerve-muscle (CBCNM) preparation. N. kuhlii, H. toshi, and P. fucus venoms caused concentration-dependent inhibition of indirect twitches in the CBCNM preparation. These three venoms also inhibited responses to exogenous acetylcholine (ACh) and carbachol (CCh), but not potassium chloride (KCl), indicating a post-synaptic mode of action. Venom from G. tricuspidata, M. cephalus, and D. tumifrons had no significant effect on indirect twitches or agonist responses in the CBCNM. Our results demonstrate that envenoming by these species of fish may result in moderate cardiovascular and/or neurotoxic effects. Future studies aimed at identifying the molecules responsible for these effects could uncover potentially novel lead compounds for future pharmaceuticals, in addition to generating new knowledge about the evolutionary relationships between venomous animals. |
format | Online Article Text |
id | pubmed-5331446 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-53314462017-03-13 The Cardiovascular and Neurotoxic Effects of the Venoms of Six Bony and Cartilaginous Fish Species Han, Han Baumann, Kate Casewell, Nicholas R. Ali, Syed A. Dobson, James Koludarov, Ivan Debono, Jordan Cutmore, Scott C. Rajapakse, Niwanthi W. Jackson, Timothy N. W. Jones, Rob Hodgson, Wayne C. Fry, Bryan G. Kuruppu, Sanjaya Toxins (Basel) Article Fish venoms are often poorly studied, in part due to the difficulty in obtaining, extracting, and storing them. In this study, we characterize the cardiovascular and neurotoxic effects of the venoms from the following six species of fish: the cartilaginous stingrays Neotrygon kuhlii and Himantura toshi, and the bony fish Platycephalus fucus, Girella tricuspidata, Mugil cephalus, and Dentex tumifrons. All venoms (10–100 µg/kg, i.v.), except G. tricuspidata and P. fuscus, induced a biphasic response on mean arterial pressure (MAP) in the anesthetised rat. P. fucus venom exhibited a hypotensive response, while venom from G. tricuspidata displayed a single depressor response. All venoms induced cardiovascular collapse at 200 µg/kg, i.v. The in vitro neurotoxic effects of venom were examined using the chick biventer cervicis nerve-muscle (CBCNM) preparation. N. kuhlii, H. toshi, and P. fucus venoms caused concentration-dependent inhibition of indirect twitches in the CBCNM preparation. These three venoms also inhibited responses to exogenous acetylcholine (ACh) and carbachol (CCh), but not potassium chloride (KCl), indicating a post-synaptic mode of action. Venom from G. tricuspidata, M. cephalus, and D. tumifrons had no significant effect on indirect twitches or agonist responses in the CBCNM. Our results demonstrate that envenoming by these species of fish may result in moderate cardiovascular and/or neurotoxic effects. Future studies aimed at identifying the molecules responsible for these effects could uncover potentially novel lead compounds for future pharmaceuticals, in addition to generating new knowledge about the evolutionary relationships between venomous animals. MDPI 2017-02-16 /pmc/articles/PMC5331446/ /pubmed/28212333 http://dx.doi.org/10.3390/toxins9020067 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Han, Han Baumann, Kate Casewell, Nicholas R. Ali, Syed A. Dobson, James Koludarov, Ivan Debono, Jordan Cutmore, Scott C. Rajapakse, Niwanthi W. Jackson, Timothy N. W. Jones, Rob Hodgson, Wayne C. Fry, Bryan G. Kuruppu, Sanjaya The Cardiovascular and Neurotoxic Effects of the Venoms of Six Bony and Cartilaginous Fish Species |
title | The Cardiovascular and Neurotoxic Effects of the Venoms of Six Bony and Cartilaginous Fish Species |
title_full | The Cardiovascular and Neurotoxic Effects of the Venoms of Six Bony and Cartilaginous Fish Species |
title_fullStr | The Cardiovascular and Neurotoxic Effects of the Venoms of Six Bony and Cartilaginous Fish Species |
title_full_unstemmed | The Cardiovascular and Neurotoxic Effects of the Venoms of Six Bony and Cartilaginous Fish Species |
title_short | The Cardiovascular and Neurotoxic Effects of the Venoms of Six Bony and Cartilaginous Fish Species |
title_sort | cardiovascular and neurotoxic effects of the venoms of six bony and cartilaginous fish species |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5331446/ https://www.ncbi.nlm.nih.gov/pubmed/28212333 http://dx.doi.org/10.3390/toxins9020067 |
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