CADEE: Computer-Aided Directed Evolution of Enzymes

The tremendous interest in enzymes as biocatalysts has led to extensive work in enzyme engineering, as well as associated methodology development. Here, a new framework for computer-aided directed evolution of enzymes (CADEE) is presented which allows a drastic reduction in the time necessary to pre...

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Autores principales: Amrein, Beat Anton, Steffen-Munsberg, Fabian, Szeler, Ireneusz, Purg, Miha, Kulkarni, Yashraj, Kamerlin, Shina Caroline Lynn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2017
Materias:
Research Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5331465/
https://www.ncbi.nlm.nih.gov/pubmed/28250941
http://dx.doi.org/10.1107/S2052252516018017
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author Amrein, Beat Anton
Steffen-Munsberg, Fabian
Szeler, Ireneusz
Purg, Miha
Kulkarni, Yashraj
Kamerlin, Shina Caroline Lynn
author_facet Amrein, Beat Anton
Steffen-Munsberg, Fabian
Szeler, Ireneusz
Purg, Miha
Kulkarni, Yashraj
Kamerlin, Shina Caroline Lynn
author_sort Amrein, Beat Anton
collection PubMed
description The tremendous interest in enzymes as biocatalysts has led to extensive work in enzyme engineering, as well as associated methodology development. Here, a new framework for computer-aided directed evolution of enzymes (CADEE) is presented which allows a drastic reduction in the time necessary to prepare and analyze in silico semi-automated directed evolution of enzymes. A pedagogical example of the application of CADEE to a real biological system is also presented in order to illustrate the CADEE workflow.
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spelling pubmed-53314652017-03-01 CADEE: Computer-Aided Directed Evolution of Enzymes Amrein, Beat Anton Steffen-Munsberg, Fabian Szeler, Ireneusz Purg, Miha Kulkarni, Yashraj Kamerlin, Shina Caroline Lynn IUCrJ Research Papers The tremendous interest in enzymes as biocatalysts has led to extensive work in enzyme engineering, as well as associated methodology development. Here, a new framework for computer-aided directed evolution of enzymes (CADEE) is presented which allows a drastic reduction in the time necessary to prepare and analyze in silico semi-automated directed evolution of enzymes. A pedagogical example of the application of CADEE to a real biological system is also presented in order to illustrate the CADEE workflow. International Union of Crystallography 2017-01-01 /pmc/articles/PMC5331465/ /pubmed/28250941 http://dx.doi.org/10.1107/S2052252516018017 Text en © Beat Anton Amrein et al. 2017 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/2.0/uk/
spellingShingle Research Papers
Amrein, Beat Anton
Steffen-Munsberg, Fabian
Szeler, Ireneusz
Purg, Miha
Kulkarni, Yashraj
Kamerlin, Shina Caroline Lynn
CADEE: Computer-Aided Directed Evolution of Enzymes
title CADEE: Computer-Aided Directed Evolution of Enzymes
title_full CADEE: Computer-Aided Directed Evolution of Enzymes
title_fullStr CADEE: Computer-Aided Directed Evolution of Enzymes
title_full_unstemmed CADEE: Computer-Aided Directed Evolution of Enzymes
title_short CADEE: Computer-Aided Directed Evolution of Enzymes
title_sort cadee: computer-aided directed evolution of enzymes
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5331465/
https://www.ncbi.nlm.nih.gov/pubmed/28250941
http://dx.doi.org/10.1107/S2052252516018017
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