A streamlined method for the design and cloning of shRNAs into an optimized Dox-inducible lentiviral vector

BACKGROUND: Short hairpin RNA (shRNA) is an established and effective tool for stable knock down of gene expression. Lentiviral vectors can be used to deliver shRNAs, thereby providing the ability to infect most mammalian cell types with high efficiency, regardless of proliferation state. Furthermor...

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Detalles Bibliográficos
Autores principales: Frank, Sander B., Schulz, Veronique V., Miranti, Cindy K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5331646/
https://www.ncbi.nlm.nih.gov/pubmed/28245848
http://dx.doi.org/10.1186/s12896-017-0341-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5331646/
https://www.ncbi.nlm.nih.gov/pubmed/28245848
http://dx.doi.org/10.1186/s12896-017-0341-x

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