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Prognostic stratification improvement by integrating ID1/ID3/IGJ gene expression signature and immunophenotypic profile in adult patients with B-ALL
BACKGROUND: Survival of adults with B-Acute Lymphoblastic Leukemia requires accurate risk stratification of patients in order to provide the appropriate therapy. Contemporary techniques, using clinical and cytogenetic variables are incomplete for prognosis prediction. METHODS: To improve the classif...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5331651/ https://www.ncbi.nlm.nih.gov/pubmed/28245840 http://dx.doi.org/10.1186/s13046-017-0506-4 |
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author | Cruz-Rodriguez, Nataly Combita, Alba L. Enciso, Leonardo J. Raney, Lauren F. Pinzon, Paula L. Lozano, Olga C. Campos, Alba M. Peñaloza, Niyireth Solano, Julio Herrera, Maria V. Zabaleta, Jovanny Quijano, Sandra |
author_facet | Cruz-Rodriguez, Nataly Combita, Alba L. Enciso, Leonardo J. Raney, Lauren F. Pinzon, Paula L. Lozano, Olga C. Campos, Alba M. Peñaloza, Niyireth Solano, Julio Herrera, Maria V. Zabaleta, Jovanny Quijano, Sandra |
author_sort | Cruz-Rodriguez, Nataly |
collection | PubMed |
description | BACKGROUND: Survival of adults with B-Acute Lymphoblastic Leukemia requires accurate risk stratification of patients in order to provide the appropriate therapy. Contemporary techniques, using clinical and cytogenetic variables are incomplete for prognosis prediction. METHODS: To improve the classification of adult patients diagnosed with B-ALL into prognosis groups, two strategies were examined and combined: the expression of the ID1/ID3/IGJ gene signature by RT-PCR and the immunophenotypic profile of 19 markers proposed in the EuroFlow protocol by Flow Cytometry in bone marrow samples. RESULTS: Both techniques were correlated to stratify patients into prognostic groups. An inverse relationship between survival and expression of the three-genes signature was observed and an immunophenotypic profile associated with clinical outcome was identified. Markers CD10 and CD20 were correlated with simultaneous overexpression of ID1, ID3 and IGJ. Patients with simultaneous expression of the poor prognosis gene signature and overexpression of CD10 or CD20, had worse Event Free Survival and Overall Survival than patients who had either the poor prognosis gene expression signature or only CD20 or CD10 overexpressed. CONCLUSION: By utilizing the combined evaluation of these two immunophenotypic markers along with the poor prognosis gene expression signature, the risk stratification can be significantly strengthened. Further studies including a large number of patients are needed to confirm these findings. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13046-017-0506-4) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5331651 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-53316512017-03-03 Prognostic stratification improvement by integrating ID1/ID3/IGJ gene expression signature and immunophenotypic profile in adult patients with B-ALL Cruz-Rodriguez, Nataly Combita, Alba L. Enciso, Leonardo J. Raney, Lauren F. Pinzon, Paula L. Lozano, Olga C. Campos, Alba M. Peñaloza, Niyireth Solano, Julio Herrera, Maria V. Zabaleta, Jovanny Quijano, Sandra J Exp Clin Cancer Res Research BACKGROUND: Survival of adults with B-Acute Lymphoblastic Leukemia requires accurate risk stratification of patients in order to provide the appropriate therapy. Contemporary techniques, using clinical and cytogenetic variables are incomplete for prognosis prediction. METHODS: To improve the classification of adult patients diagnosed with B-ALL into prognosis groups, two strategies were examined and combined: the expression of the ID1/ID3/IGJ gene signature by RT-PCR and the immunophenotypic profile of 19 markers proposed in the EuroFlow protocol by Flow Cytometry in bone marrow samples. RESULTS: Both techniques were correlated to stratify patients into prognostic groups. An inverse relationship between survival and expression of the three-genes signature was observed and an immunophenotypic profile associated with clinical outcome was identified. Markers CD10 and CD20 were correlated with simultaneous overexpression of ID1, ID3 and IGJ. Patients with simultaneous expression of the poor prognosis gene signature and overexpression of CD10 or CD20, had worse Event Free Survival and Overall Survival than patients who had either the poor prognosis gene expression signature or only CD20 or CD10 overexpressed. CONCLUSION: By utilizing the combined evaluation of these two immunophenotypic markers along with the poor prognosis gene expression signature, the risk stratification can be significantly strengthened. Further studies including a large number of patients are needed to confirm these findings. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13046-017-0506-4) contains supplementary material, which is available to authorized users. BioMed Central 2017-02-28 /pmc/articles/PMC5331651/ /pubmed/28245840 http://dx.doi.org/10.1186/s13046-017-0506-4 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Cruz-Rodriguez, Nataly Combita, Alba L. Enciso, Leonardo J. Raney, Lauren F. Pinzon, Paula L. Lozano, Olga C. Campos, Alba M. Peñaloza, Niyireth Solano, Julio Herrera, Maria V. Zabaleta, Jovanny Quijano, Sandra Prognostic stratification improvement by integrating ID1/ID3/IGJ gene expression signature and immunophenotypic profile in adult patients with B-ALL |
title | Prognostic stratification improvement by integrating ID1/ID3/IGJ gene expression signature and immunophenotypic profile in adult patients with B-ALL |
title_full | Prognostic stratification improvement by integrating ID1/ID3/IGJ gene expression signature and immunophenotypic profile in adult patients with B-ALL |
title_fullStr | Prognostic stratification improvement by integrating ID1/ID3/IGJ gene expression signature and immunophenotypic profile in adult patients with B-ALL |
title_full_unstemmed | Prognostic stratification improvement by integrating ID1/ID3/IGJ gene expression signature and immunophenotypic profile in adult patients with B-ALL |
title_short | Prognostic stratification improvement by integrating ID1/ID3/IGJ gene expression signature and immunophenotypic profile in adult patients with B-ALL |
title_sort | prognostic stratification improvement by integrating id1/id3/igj gene expression signature and immunophenotypic profile in adult patients with b-all |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5331651/ https://www.ncbi.nlm.nih.gov/pubmed/28245840 http://dx.doi.org/10.1186/s13046-017-0506-4 |
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