Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP

BACKGROUND: While NGS allows rapid global detection of transcripts, it remains difficult to distinguish ncRNAs from short mRNAs. To detect potentially translated RNAs, we developed an improved protocol for bacterial ribosomal footprinting (RIBOseq). This allowed distinguishing ncRNA from mRNA in EHE...

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Detalles Bibliográficos
Autores principales: Neuhaus, Klaus, Landstorfer, Richard, Simon, Svenja, Schober, Steffen, Wright, Patrick R., Smith, Cameron, Backofen, Rolf, Wecko, Romy, Keim, Daniel A., Scherer, Siegfried
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5331693/
https://www.ncbi.nlm.nih.gov/pubmed/28245801
http://dx.doi.org/10.1186/s12864-017-3586-9

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