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Genome-wide characterization and expression profiling of HD-Zip gene family related to abiotic stress in cassava
Homeodomain-leucine zipper (HD-Zip) gene family plays important roles in various abiotic stresses and hormone signaling in plants. However, no information is currently available regarding this family in cassava (Manihot esculenta), an important drought-tolerant crop in tropical and sub-tropical area...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5332091/ https://www.ncbi.nlm.nih.gov/pubmed/28249019 http://dx.doi.org/10.1371/journal.pone.0173043 |
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author | Ding, Zehong Fu, Lili Yan, Yan Tie, Weiwei Xia, Zhiqiang Wang, Wenquan Peng, Ming Hu, Wei Zhang, Jiaming |
author_facet | Ding, Zehong Fu, Lili Yan, Yan Tie, Weiwei Xia, Zhiqiang Wang, Wenquan Peng, Ming Hu, Wei Zhang, Jiaming |
author_sort | Ding, Zehong |
collection | PubMed |
description | Homeodomain-leucine zipper (HD-Zip) gene family plays important roles in various abiotic stresses and hormone signaling in plants. However, no information is currently available regarding this family in cassava (Manihot esculenta), an important drought-tolerant crop in tropical and sub-tropical areas. Here, 57 HD-Zip genes (MeHDZ01-57) were identified in the cassava genome, and they were classified into four subfamilies based on phylogenetic analysis, which was further supported by their gene structure and conserved motif characteristics. Of which five gene pairs were involved in segmental duplication but none for tandem duplication, suggesting that segmental duplication was the main cause for the expansion of MeHDZ gene family in cassava. Global expression profiles revealed that MeHDZ genes were constitutively expressed, or not expressed, or tissue-specific expressed in examined tissues in both cultivated and wild subspecies. Transcriptomic analysis of three genotypes showed that most of MeHDZ genes responded differently to drought and polyethylene glycol treatments. Subsequently, quantitative RT-PCR analysis revealed comprehensive responses of twelve selected MeHDZ genes to various stimuli including cold, salt, and ABA treatments. These findings will increase our understanding of HD-Zip gene family involved in abiotic stresses and signaling transduction, and will provide a solid base for further functional characterization of MeHDZ genes in cassava. |
format | Online Article Text |
id | pubmed-5332091 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-53320912017-03-10 Genome-wide characterization and expression profiling of HD-Zip gene family related to abiotic stress in cassava Ding, Zehong Fu, Lili Yan, Yan Tie, Weiwei Xia, Zhiqiang Wang, Wenquan Peng, Ming Hu, Wei Zhang, Jiaming PLoS One Research Article Homeodomain-leucine zipper (HD-Zip) gene family plays important roles in various abiotic stresses and hormone signaling in plants. However, no information is currently available regarding this family in cassava (Manihot esculenta), an important drought-tolerant crop in tropical and sub-tropical areas. Here, 57 HD-Zip genes (MeHDZ01-57) were identified in the cassava genome, and they were classified into four subfamilies based on phylogenetic analysis, which was further supported by their gene structure and conserved motif characteristics. Of which five gene pairs were involved in segmental duplication but none for tandem duplication, suggesting that segmental duplication was the main cause for the expansion of MeHDZ gene family in cassava. Global expression profiles revealed that MeHDZ genes were constitutively expressed, or not expressed, or tissue-specific expressed in examined tissues in both cultivated and wild subspecies. Transcriptomic analysis of three genotypes showed that most of MeHDZ genes responded differently to drought and polyethylene glycol treatments. Subsequently, quantitative RT-PCR analysis revealed comprehensive responses of twelve selected MeHDZ genes to various stimuli including cold, salt, and ABA treatments. These findings will increase our understanding of HD-Zip gene family involved in abiotic stresses and signaling transduction, and will provide a solid base for further functional characterization of MeHDZ genes in cassava. Public Library of Science 2017-03-01 /pmc/articles/PMC5332091/ /pubmed/28249019 http://dx.doi.org/10.1371/journal.pone.0173043 Text en © 2017 Ding et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Ding, Zehong Fu, Lili Yan, Yan Tie, Weiwei Xia, Zhiqiang Wang, Wenquan Peng, Ming Hu, Wei Zhang, Jiaming Genome-wide characterization and expression profiling of HD-Zip gene family related to abiotic stress in cassava |
title | Genome-wide characterization and expression profiling of HD-Zip gene family related to abiotic stress in cassava |
title_full | Genome-wide characterization and expression profiling of HD-Zip gene family related to abiotic stress in cassava |
title_fullStr | Genome-wide characterization and expression profiling of HD-Zip gene family related to abiotic stress in cassava |
title_full_unstemmed | Genome-wide characterization and expression profiling of HD-Zip gene family related to abiotic stress in cassava |
title_short | Genome-wide characterization and expression profiling of HD-Zip gene family related to abiotic stress in cassava |
title_sort | genome-wide characterization and expression profiling of hd-zip gene family related to abiotic stress in cassava |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5332091/ https://www.ncbi.nlm.nih.gov/pubmed/28249019 http://dx.doi.org/10.1371/journal.pone.0173043 |
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