Cargando…

Exploring gastric bacterial community in young pigs

Microbiota plays an important role in the homeostasis of the gastrointestinal tract. Understanding the variations of the commensal microbiota composition is crucial for a more efficient control of enteric infectious diseases and for the reduction of the use of antibiotics in animal production, which...

Descripción completa

Detalles Bibliográficos
Autores principales: Motta, Vincenzo, Trevisi, Paolo, Bertolini, Francesca, Ribani, Anisa, Schiavo, Giuseppina, Fontanesi, Luca, Bosi, Paolo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5332105/
https://www.ncbi.nlm.nih.gov/pubmed/28249050
http://dx.doi.org/10.1371/journal.pone.0173029
_version_ 1782511492975820800
author Motta, Vincenzo
Trevisi, Paolo
Bertolini, Francesca
Ribani, Anisa
Schiavo, Giuseppina
Fontanesi, Luca
Bosi, Paolo
author_facet Motta, Vincenzo
Trevisi, Paolo
Bertolini, Francesca
Ribani, Anisa
Schiavo, Giuseppina
Fontanesi, Luca
Bosi, Paolo
author_sort Motta, Vincenzo
collection PubMed
description Microbiota plays an important role in the homeostasis of the gastrointestinal tract. Understanding the variations of the commensal microbiota composition is crucial for a more efficient control of enteric infectious diseases and for the reduction of the use of antibiotics in animal production, which are the main points of interest for improved animal healthcare and welfare and for consumer health protection. Even though the intestinal microbiota has been extensively studied, little is known about the gastric microbiota. This pilot study was aimed at a descriptive analysis of the gastric microbiota in healthy pigs and at the identification of any differences among four potentially distinct microbial niches in the stomach. Gastric mucosal samples from the oxyntic area, the pylorus and the gastric groove, and a sample of gastric contents were collected from four healthy weaned pigs. Bacterial DNA was isolated and extracted from each sample and amplicons from the V6 region of the 16S rRNA gene were sequenced using Ion Torrent PGM. The data were analysed by an “unsupervised” and a “supervised” approach in the Ribosomal Database Project (RDP) pipeline. Proteobacteria was the dominant phylum in all the samples. Differences in bacterial community composition were found between mucosal and content samples (one-way ANOSIM pairwise post hoc test, p < 0.05); instead, the different mucosal regions did not show differences between them. The mucosal samples were characterised by Herbiconiux and Brevundimonas, two genera which include cellulolytic and xylanolytic strains. Nevertheless, additional larger trials are needed to support the data presented in this pilot study and to increase the knowledge regarding the resident microbiota of the stomach.
format Online
Article
Text
id pubmed-5332105
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-53321052017-03-10 Exploring gastric bacterial community in young pigs Motta, Vincenzo Trevisi, Paolo Bertolini, Francesca Ribani, Anisa Schiavo, Giuseppina Fontanesi, Luca Bosi, Paolo PLoS One Research Article Microbiota plays an important role in the homeostasis of the gastrointestinal tract. Understanding the variations of the commensal microbiota composition is crucial for a more efficient control of enteric infectious diseases and for the reduction of the use of antibiotics in animal production, which are the main points of interest for improved animal healthcare and welfare and for consumer health protection. Even though the intestinal microbiota has been extensively studied, little is known about the gastric microbiota. This pilot study was aimed at a descriptive analysis of the gastric microbiota in healthy pigs and at the identification of any differences among four potentially distinct microbial niches in the stomach. Gastric mucosal samples from the oxyntic area, the pylorus and the gastric groove, and a sample of gastric contents were collected from four healthy weaned pigs. Bacterial DNA was isolated and extracted from each sample and amplicons from the V6 region of the 16S rRNA gene were sequenced using Ion Torrent PGM. The data were analysed by an “unsupervised” and a “supervised” approach in the Ribosomal Database Project (RDP) pipeline. Proteobacteria was the dominant phylum in all the samples. Differences in bacterial community composition were found between mucosal and content samples (one-way ANOSIM pairwise post hoc test, p < 0.05); instead, the different mucosal regions did not show differences between them. The mucosal samples were characterised by Herbiconiux and Brevundimonas, two genera which include cellulolytic and xylanolytic strains. Nevertheless, additional larger trials are needed to support the data presented in this pilot study and to increase the knowledge regarding the resident microbiota of the stomach. Public Library of Science 2017-03-01 /pmc/articles/PMC5332105/ /pubmed/28249050 http://dx.doi.org/10.1371/journal.pone.0173029 Text en © 2017 Motta et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Motta, Vincenzo
Trevisi, Paolo
Bertolini, Francesca
Ribani, Anisa
Schiavo, Giuseppina
Fontanesi, Luca
Bosi, Paolo
Exploring gastric bacterial community in young pigs
title Exploring gastric bacterial community in young pigs
title_full Exploring gastric bacterial community in young pigs
title_fullStr Exploring gastric bacterial community in young pigs
title_full_unstemmed Exploring gastric bacterial community in young pigs
title_short Exploring gastric bacterial community in young pigs
title_sort exploring gastric bacterial community in young pigs
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5332105/
https://www.ncbi.nlm.nih.gov/pubmed/28249050
http://dx.doi.org/10.1371/journal.pone.0173029
work_keys_str_mv AT mottavincenzo exploringgastricbacterialcommunityinyoungpigs
AT trevisipaolo exploringgastricbacterialcommunityinyoungpigs
AT bertolinifrancesca exploringgastricbacterialcommunityinyoungpigs
AT ribanianisa exploringgastricbacterialcommunityinyoungpigs
AT schiavogiuseppina exploringgastricbacterialcommunityinyoungpigs
AT fontanesiluca exploringgastricbacterialcommunityinyoungpigs
AT bosipaolo exploringgastricbacterialcommunityinyoungpigs