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Metabolic changes in the midgut of Eri silkworm after Oral administration of 1-deoxynojirimycin: A (1)H-NMR-based metabonomic study
1-deoxynojirimycin (DNJ) is a natural D-glucose analogue and has a strong physiological activity in inhibiting α-glucosidase in vivo. The antidiabetic effects of DNJ in mice or other mammals were extensively explored, but the physiological and toxic roles of DNJ in insects was seldom reported. In th...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5332107/ https://www.ncbi.nlm.nih.gov/pubmed/28249023 http://dx.doi.org/10.1371/journal.pone.0173213 |
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author | Deng, Ming-Jie Lin, Xiao-Dong Wen, Chao-Wei Dong, Min-Jian Lin, Qiu-Ting Zhang, Shang-Zhi Xu, Jia-Ping |
author_facet | Deng, Ming-Jie Lin, Xiao-Dong Wen, Chao-Wei Dong, Min-Jian Lin, Qiu-Ting Zhang, Shang-Zhi Xu, Jia-Ping |
author_sort | Deng, Ming-Jie |
collection | PubMed |
description | 1-deoxynojirimycin (DNJ) is a natural D-glucose analogue and has a strong physiological activity in inhibiting α-glucosidase in vivo. The antidiabetic effects of DNJ in mice or other mammals were extensively explored, but the physiological and toxic roles of DNJ in insects was seldom reported. In this study, the biological effects of DNJ were examined in midgut extracts of fourth-instar larvae of Eri silkworm (Samia cynthia ricini, Saturniidae). Based on nuclear magnetic resonance (NMR) metabonomics technology, we analyzed the alterations of glycometabolism, lipids, and energy metabolism pathways in the midgut of S. cynthia ricini caused by DNJ. Pattern recognition analysis (partial least square-discriminant analysis, PLS-DA) showed that four groups of latex, 0.25% DNJ, 0.5% DNJ and the mixture of 0.5% DNJ and latex (1:1) were distinctly different from the control group. Moreover, several metabolic pathways of DNJ-mediated modulation in the midgut were identified. Compared with the control group, alanine, succinate, glutamate, and fumarate concentrations decreased in three groups of 0.5% DNJ, latex, and the mixture, choline levels increased in two DNJ groups, and trehalose levels increased in all experimental groups. Therefore, these results suggest that DNJ modulated lipid metabolism by limiting the hydrolysis pathways of phospholipids metabolism. Additionally, DNJ has a potent negative effect on energy metabolism by inhibiting the hydrolysis of trehalose, glycolysis and the tricarboxylic acid (TCA) cycle. Overall, DNJ, as a single-ingredient, is an efficient substance for modulating lipid metabolism and inhibiting energy metabolism. |
format | Online Article Text |
id | pubmed-5332107 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-53321072017-03-10 Metabolic changes in the midgut of Eri silkworm after Oral administration of 1-deoxynojirimycin: A (1)H-NMR-based metabonomic study Deng, Ming-Jie Lin, Xiao-Dong Wen, Chao-Wei Dong, Min-Jian Lin, Qiu-Ting Zhang, Shang-Zhi Xu, Jia-Ping PLoS One Research Article 1-deoxynojirimycin (DNJ) is a natural D-glucose analogue and has a strong physiological activity in inhibiting α-glucosidase in vivo. The antidiabetic effects of DNJ in mice or other mammals were extensively explored, but the physiological and toxic roles of DNJ in insects was seldom reported. In this study, the biological effects of DNJ were examined in midgut extracts of fourth-instar larvae of Eri silkworm (Samia cynthia ricini, Saturniidae). Based on nuclear magnetic resonance (NMR) metabonomics technology, we analyzed the alterations of glycometabolism, lipids, and energy metabolism pathways in the midgut of S. cynthia ricini caused by DNJ. Pattern recognition analysis (partial least square-discriminant analysis, PLS-DA) showed that four groups of latex, 0.25% DNJ, 0.5% DNJ and the mixture of 0.5% DNJ and latex (1:1) were distinctly different from the control group. Moreover, several metabolic pathways of DNJ-mediated modulation in the midgut were identified. Compared with the control group, alanine, succinate, glutamate, and fumarate concentrations decreased in three groups of 0.5% DNJ, latex, and the mixture, choline levels increased in two DNJ groups, and trehalose levels increased in all experimental groups. Therefore, these results suggest that DNJ modulated lipid metabolism by limiting the hydrolysis pathways of phospholipids metabolism. Additionally, DNJ has a potent negative effect on energy metabolism by inhibiting the hydrolysis of trehalose, glycolysis and the tricarboxylic acid (TCA) cycle. Overall, DNJ, as a single-ingredient, is an efficient substance for modulating lipid metabolism and inhibiting energy metabolism. Public Library of Science 2017-03-01 /pmc/articles/PMC5332107/ /pubmed/28249023 http://dx.doi.org/10.1371/journal.pone.0173213 Text en © 2017 Deng et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Deng, Ming-Jie Lin, Xiao-Dong Wen, Chao-Wei Dong, Min-Jian Lin, Qiu-Ting Zhang, Shang-Zhi Xu, Jia-Ping Metabolic changes in the midgut of Eri silkworm after Oral administration of 1-deoxynojirimycin: A (1)H-NMR-based metabonomic study |
title | Metabolic changes in the midgut of Eri silkworm after Oral administration of 1-deoxynojirimycin: A (1)H-NMR-based metabonomic study |
title_full | Metabolic changes in the midgut of Eri silkworm after Oral administration of 1-deoxynojirimycin: A (1)H-NMR-based metabonomic study |
title_fullStr | Metabolic changes in the midgut of Eri silkworm after Oral administration of 1-deoxynojirimycin: A (1)H-NMR-based metabonomic study |
title_full_unstemmed | Metabolic changes in the midgut of Eri silkworm after Oral administration of 1-deoxynojirimycin: A (1)H-NMR-based metabonomic study |
title_short | Metabolic changes in the midgut of Eri silkworm after Oral administration of 1-deoxynojirimycin: A (1)H-NMR-based metabonomic study |
title_sort | metabolic changes in the midgut of eri silkworm after oral administration of 1-deoxynojirimycin: a (1)h-nmr-based metabonomic study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5332107/ https://www.ncbi.nlm.nih.gov/pubmed/28249023 http://dx.doi.org/10.1371/journal.pone.0173213 |
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