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A Twist2-Dependent Progenitor Cell Contributes to Adult Skeletal Muscle
Skeletal muscle possesses remarkable regenerative potential due to satellite cells, an injury-responsive stem cell population located beneath the muscle basal lamina that expresses Pax7. By lineage tracing of progenitor cells expressing the Twist2 (Tw2) transcription factor in mice, we discovered a...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5332283/ https://www.ncbi.nlm.nih.gov/pubmed/28218909 http://dx.doi.org/10.1038/ncb3477 |
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author | Liu, Ning Garry, Glynnis A. Li, Stephen Bezprozvannaya, Svetlana Sanchez-Ortiz, Efrain Chen, Beibei Shelton, John M. Jaichander, Priscilla Bassel-Duby, Rhonda Olson, Eric N. |
author_facet | Liu, Ning Garry, Glynnis A. Li, Stephen Bezprozvannaya, Svetlana Sanchez-Ortiz, Efrain Chen, Beibei Shelton, John M. Jaichander, Priscilla Bassel-Duby, Rhonda Olson, Eric N. |
author_sort | Liu, Ning |
collection | PubMed |
description | Skeletal muscle possesses remarkable regenerative potential due to satellite cells, an injury-responsive stem cell population located beneath the muscle basal lamina that expresses Pax7. By lineage tracing of progenitor cells expressing the Twist2 (Tw2) transcription factor in mice, we discovered a myogenic lineage that resides outside the basal lamina of adult skeletal muscle. Tw2+ progenitors are molecularly and anatomically distinct from satellite cells, are highly myogenic in vitro, and can fuse with themselves and with satellite cells. Tw2+ progenitors contribute specifically to type IIb/x myofibers during adulthood and muscle regeneration, and their genetic ablation causes wasting of type IIb myofibers. We show that Tw2 expression maintains progenitor cells in an undifferentiated state that is poised to initiate myogenesis in response to appropriate cues that extinguish Tw2 expression. Tw2-expressing myogenic progenitors represent a previously unrecognized, fiber-type specific stem cell involved in post-natal muscle growth and regeneration. |
format | Online Article Text |
id | pubmed-5332283 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
record_format | MEDLINE/PubMed |
spelling | pubmed-53322832017-08-20 A Twist2-Dependent Progenitor Cell Contributes to Adult Skeletal Muscle Liu, Ning Garry, Glynnis A. Li, Stephen Bezprozvannaya, Svetlana Sanchez-Ortiz, Efrain Chen, Beibei Shelton, John M. Jaichander, Priscilla Bassel-Duby, Rhonda Olson, Eric N. Nat Cell Biol Article Skeletal muscle possesses remarkable regenerative potential due to satellite cells, an injury-responsive stem cell population located beneath the muscle basal lamina that expresses Pax7. By lineage tracing of progenitor cells expressing the Twist2 (Tw2) transcription factor in mice, we discovered a myogenic lineage that resides outside the basal lamina of adult skeletal muscle. Tw2+ progenitors are molecularly and anatomically distinct from satellite cells, are highly myogenic in vitro, and can fuse with themselves and with satellite cells. Tw2+ progenitors contribute specifically to type IIb/x myofibers during adulthood and muscle regeneration, and their genetic ablation causes wasting of type IIb myofibers. We show that Tw2 expression maintains progenitor cells in an undifferentiated state that is poised to initiate myogenesis in response to appropriate cues that extinguish Tw2 expression. Tw2-expressing myogenic progenitors represent a previously unrecognized, fiber-type specific stem cell involved in post-natal muscle growth and regeneration. 2017-02-20 2017-03 /pmc/articles/PMC5332283/ /pubmed/28218909 http://dx.doi.org/10.1038/ncb3477 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Liu, Ning Garry, Glynnis A. Li, Stephen Bezprozvannaya, Svetlana Sanchez-Ortiz, Efrain Chen, Beibei Shelton, John M. Jaichander, Priscilla Bassel-Duby, Rhonda Olson, Eric N. A Twist2-Dependent Progenitor Cell Contributes to Adult Skeletal Muscle |
title | A Twist2-Dependent Progenitor Cell Contributes to Adult Skeletal Muscle |
title_full | A Twist2-Dependent Progenitor Cell Contributes to Adult Skeletal Muscle |
title_fullStr | A Twist2-Dependent Progenitor Cell Contributes to Adult Skeletal Muscle |
title_full_unstemmed | A Twist2-Dependent Progenitor Cell Contributes to Adult Skeletal Muscle |
title_short | A Twist2-Dependent Progenitor Cell Contributes to Adult Skeletal Muscle |
title_sort | twist2-dependent progenitor cell contributes to adult skeletal muscle |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5332283/ https://www.ncbi.nlm.nih.gov/pubmed/28218909 http://dx.doi.org/10.1038/ncb3477 |
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