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Identifying DCN and HSPD1 as Potential Biomarkers in Colon Cancer Using 2D-LC-MS/MS Combined with iTRAQ Technology
Colon cancer is one of the most common types of gastrointestinal cancers and the fourth cause of cancer death worldwide. To discover novel diagnostic biomarkers for colon cancer and investigate potential mechanisms of oncogenesis, quantitative proteomic approach using iTRAQ-tagging and 2D-LC-MS/MS w...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5332900/ https://www.ncbi.nlm.nih.gov/pubmed/28261350 http://dx.doi.org/10.7150/jca.17192 |
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author | Li, Guoqing Li, Maoyu Liang, Xujun Xiao, Zhefeng Zhang, Pengfei Shao, Meiying Peng, Fang Chen, Yongheng Li, Yuanyuan Chen, Zhuchu |
author_facet | Li, Guoqing Li, Maoyu Liang, Xujun Xiao, Zhefeng Zhang, Pengfei Shao, Meiying Peng, Fang Chen, Yongheng Li, Yuanyuan Chen, Zhuchu |
author_sort | Li, Guoqing |
collection | PubMed |
description | Colon cancer is one of the most common types of gastrointestinal cancers and the fourth cause of cancer death worldwide. To discover novel diagnostic biomarkers for colon cancer and investigate potential mechanisms of oncogenesis, quantitative proteomic approach using iTRAQ-tagging and 2D-LC-MS/MS was performed to characterize proteins alterations in colon cancer and non-neoplastic colonic mucosa (NNCM) using laser capture microdissection-harvested from the two types of tissues, respectively. As a result, 188 DEPs were identified, and the differential expression of two DEPs (DCN and HSPD1) was further verified by Western blotting and immunohistochemistry. KEGG pathway analysis disclosed that the DEPs were related to signaling pathways associated with cancer; furthermore, DCN and HSPD1 are in the relative central hub position among protein-protein interaction subnetwork of the DEPs. The results not only shed light on the mechanism by the DEPs contributed to colonic carcinogenesis, but also showed that DCN and HSPD1 are novel potential biomarkers for the diagnosis of colon cancer. |
format | Online Article Text |
id | pubmed-5332900 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-53329002017-03-03 Identifying DCN and HSPD1 as Potential Biomarkers in Colon Cancer Using 2D-LC-MS/MS Combined with iTRAQ Technology Li, Guoqing Li, Maoyu Liang, Xujun Xiao, Zhefeng Zhang, Pengfei Shao, Meiying Peng, Fang Chen, Yongheng Li, Yuanyuan Chen, Zhuchu J Cancer Research Paper Colon cancer is one of the most common types of gastrointestinal cancers and the fourth cause of cancer death worldwide. To discover novel diagnostic biomarkers for colon cancer and investigate potential mechanisms of oncogenesis, quantitative proteomic approach using iTRAQ-tagging and 2D-LC-MS/MS was performed to characterize proteins alterations in colon cancer and non-neoplastic colonic mucosa (NNCM) using laser capture microdissection-harvested from the two types of tissues, respectively. As a result, 188 DEPs were identified, and the differential expression of two DEPs (DCN and HSPD1) was further verified by Western blotting and immunohistochemistry. KEGG pathway analysis disclosed that the DEPs were related to signaling pathways associated with cancer; furthermore, DCN and HSPD1 are in the relative central hub position among protein-protein interaction subnetwork of the DEPs. The results not only shed light on the mechanism by the DEPs contributed to colonic carcinogenesis, but also showed that DCN and HSPD1 are novel potential biomarkers for the diagnosis of colon cancer. Ivyspring International Publisher 2017-02-11 /pmc/articles/PMC5332900/ /pubmed/28261350 http://dx.doi.org/10.7150/jca.17192 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Li, Guoqing Li, Maoyu Liang, Xujun Xiao, Zhefeng Zhang, Pengfei Shao, Meiying Peng, Fang Chen, Yongheng Li, Yuanyuan Chen, Zhuchu Identifying DCN and HSPD1 as Potential Biomarkers in Colon Cancer Using 2D-LC-MS/MS Combined with iTRAQ Technology |
title | Identifying DCN and HSPD1 as Potential Biomarkers in Colon Cancer Using 2D-LC-MS/MS Combined with iTRAQ Technology |
title_full | Identifying DCN and HSPD1 as Potential Biomarkers in Colon Cancer Using 2D-LC-MS/MS Combined with iTRAQ Technology |
title_fullStr | Identifying DCN and HSPD1 as Potential Biomarkers in Colon Cancer Using 2D-LC-MS/MS Combined with iTRAQ Technology |
title_full_unstemmed | Identifying DCN and HSPD1 as Potential Biomarkers in Colon Cancer Using 2D-LC-MS/MS Combined with iTRAQ Technology |
title_short | Identifying DCN and HSPD1 as Potential Biomarkers in Colon Cancer Using 2D-LC-MS/MS Combined with iTRAQ Technology |
title_sort | identifying dcn and hspd1 as potential biomarkers in colon cancer using 2d-lc-ms/ms combined with itraq technology |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5332900/ https://www.ncbi.nlm.nih.gov/pubmed/28261350 http://dx.doi.org/10.7150/jca.17192 |
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