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Genetic Assessment of African Swine Fever Isolates Involved in Outbreaks in the Democratic Republic of Congo between 2005 and 2012 Reveals Co-Circulation of p72 Genotypes I, IX and XIV, Including 19 Variants
African swine fever (ASF) is a devastating disease of domestic pigs. It is a socioeconomically important disease, initially described from Kenya, but subsequently reported in most Sub-Saharan countries. ASF spread to Europe, South America and the Caribbean through multiple introductions which were i...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5332950/ https://www.ncbi.nlm.nih.gov/pubmed/28218698 http://dx.doi.org/10.3390/v9020031 |
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author | Mulumba–Mfumu, Leopold K. Achenbach, Jenna E. Mauldin, Matthew R. Dixon, Linda K. Tshilenge, Curé Georges Thiry, Etienne Moreno, Noelia Blanco, Esther Saegerman, Claude Lamien, Charles E. Diallo, Adama |
author_facet | Mulumba–Mfumu, Leopold K. Achenbach, Jenna E. Mauldin, Matthew R. Dixon, Linda K. Tshilenge, Curé Georges Thiry, Etienne Moreno, Noelia Blanco, Esther Saegerman, Claude Lamien, Charles E. Diallo, Adama |
author_sort | Mulumba–Mfumu, Leopold K. |
collection | PubMed |
description | African swine fever (ASF) is a devastating disease of domestic pigs. It is a socioeconomically important disease, initially described from Kenya, but subsequently reported in most Sub-Saharan countries. ASF spread to Europe, South America and the Caribbean through multiple introductions which were initially eradicated—except for Sardinia—followed by re‑introduction into Europe in 2007. In this study of ASF within the Democratic Republic of the Congo, 62 domestic pig samples, collected between 2005–2012, were examined for viral DNA and sequencing at multiple loci: C-terminus of the B646L gene (p72 protein), central hypervariable region (CVR) of the B602L gene, and the E183L gene (p54 protein). Phylogenetic analyses identified three circulating genotypes: I (64.5% of samples), IX (32.3%), and XIV (3.2%). This is the first evidence of genotypes IX and XIV within this country. Examination of the CVR revealed high levels of intra-genotypic variation, with 19 identified variants. |
format | Online Article Text |
id | pubmed-5332950 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-53329502017-03-13 Genetic Assessment of African Swine Fever Isolates Involved in Outbreaks in the Democratic Republic of Congo between 2005 and 2012 Reveals Co-Circulation of p72 Genotypes I, IX and XIV, Including 19 Variants Mulumba–Mfumu, Leopold K. Achenbach, Jenna E. Mauldin, Matthew R. Dixon, Linda K. Tshilenge, Curé Georges Thiry, Etienne Moreno, Noelia Blanco, Esther Saegerman, Claude Lamien, Charles E. Diallo, Adama Viruses Article African swine fever (ASF) is a devastating disease of domestic pigs. It is a socioeconomically important disease, initially described from Kenya, but subsequently reported in most Sub-Saharan countries. ASF spread to Europe, South America and the Caribbean through multiple introductions which were initially eradicated—except for Sardinia—followed by re‑introduction into Europe in 2007. In this study of ASF within the Democratic Republic of the Congo, 62 domestic pig samples, collected between 2005–2012, were examined for viral DNA and sequencing at multiple loci: C-terminus of the B646L gene (p72 protein), central hypervariable region (CVR) of the B602L gene, and the E183L gene (p54 protein). Phylogenetic analyses identified three circulating genotypes: I (64.5% of samples), IX (32.3%), and XIV (3.2%). This is the first evidence of genotypes IX and XIV within this country. Examination of the CVR revealed high levels of intra-genotypic variation, with 19 identified variants. MDPI 2017-02-18 /pmc/articles/PMC5332950/ /pubmed/28218698 http://dx.doi.org/10.3390/v9020031 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mulumba–Mfumu, Leopold K. Achenbach, Jenna E. Mauldin, Matthew R. Dixon, Linda K. Tshilenge, Curé Georges Thiry, Etienne Moreno, Noelia Blanco, Esther Saegerman, Claude Lamien, Charles E. Diallo, Adama Genetic Assessment of African Swine Fever Isolates Involved in Outbreaks in the Democratic Republic of Congo between 2005 and 2012 Reveals Co-Circulation of p72 Genotypes I, IX and XIV, Including 19 Variants |
title | Genetic Assessment of African Swine Fever Isolates Involved in Outbreaks in the Democratic Republic of Congo between 2005 and 2012 Reveals Co-Circulation of p72 Genotypes I, IX and XIV, Including 19 Variants |
title_full | Genetic Assessment of African Swine Fever Isolates Involved in Outbreaks in the Democratic Republic of Congo between 2005 and 2012 Reveals Co-Circulation of p72 Genotypes I, IX and XIV, Including 19 Variants |
title_fullStr | Genetic Assessment of African Swine Fever Isolates Involved in Outbreaks in the Democratic Republic of Congo between 2005 and 2012 Reveals Co-Circulation of p72 Genotypes I, IX and XIV, Including 19 Variants |
title_full_unstemmed | Genetic Assessment of African Swine Fever Isolates Involved in Outbreaks in the Democratic Republic of Congo between 2005 and 2012 Reveals Co-Circulation of p72 Genotypes I, IX and XIV, Including 19 Variants |
title_short | Genetic Assessment of African Swine Fever Isolates Involved in Outbreaks in the Democratic Republic of Congo between 2005 and 2012 Reveals Co-Circulation of p72 Genotypes I, IX and XIV, Including 19 Variants |
title_sort | genetic assessment of african swine fever isolates involved in outbreaks in the democratic republic of congo between 2005 and 2012 reveals co-circulation of p72 genotypes i, ix and xiv, including 19 variants |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5332950/ https://www.ncbi.nlm.nih.gov/pubmed/28218698 http://dx.doi.org/10.3390/v9020031 |
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