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Genetic Assessment of African Swine Fever Isolates Involved in Outbreaks in the Democratic Republic of Congo between 2005 and 2012 Reveals Co-Circulation of p72 Genotypes I, IX and XIV, Including 19 Variants

African swine fever (ASF) is a devastating disease of domestic pigs. It is a socioeconomically important disease, initially described from Kenya, but subsequently reported in most Sub-Saharan countries. ASF spread to Europe, South America and the Caribbean through multiple introductions which were i...

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Autores principales: Mulumba–Mfumu, Leopold K., Achenbach, Jenna E., Mauldin, Matthew R., Dixon, Linda K., Tshilenge, Curé Georges, Thiry, Etienne, Moreno, Noelia, Blanco, Esther, Saegerman, Claude, Lamien, Charles E., Diallo, Adama
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5332950/
https://www.ncbi.nlm.nih.gov/pubmed/28218698
http://dx.doi.org/10.3390/v9020031
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author Mulumba–Mfumu, Leopold K.
Achenbach, Jenna E.
Mauldin, Matthew R.
Dixon, Linda K.
Tshilenge, Curé Georges
Thiry, Etienne
Moreno, Noelia
Blanco, Esther
Saegerman, Claude
Lamien, Charles E.
Diallo, Adama
author_facet Mulumba–Mfumu, Leopold K.
Achenbach, Jenna E.
Mauldin, Matthew R.
Dixon, Linda K.
Tshilenge, Curé Georges
Thiry, Etienne
Moreno, Noelia
Blanco, Esther
Saegerman, Claude
Lamien, Charles E.
Diallo, Adama
author_sort Mulumba–Mfumu, Leopold K.
collection PubMed
description African swine fever (ASF) is a devastating disease of domestic pigs. It is a socioeconomically important disease, initially described from Kenya, but subsequently reported in most Sub-Saharan countries. ASF spread to Europe, South America and the Caribbean through multiple introductions which were initially eradicated—except for Sardinia—followed by re‑introduction into Europe in 2007. In this study of ASF within the Democratic Republic of the Congo, 62 domestic pig samples, collected between 2005–2012, were examined for viral DNA and sequencing at multiple loci: C-terminus of the B646L gene (p72 protein), central hypervariable region (CVR) of the B602L gene, and the E183L gene (p54 protein). Phylogenetic analyses identified three circulating genotypes: I (64.5% of samples), IX (32.3%), and XIV (3.2%). This is the first evidence of genotypes IX and XIV within this country. Examination of the CVR revealed high levels of intra-genotypic variation, with 19 identified variants.
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spelling pubmed-53329502017-03-13 Genetic Assessment of African Swine Fever Isolates Involved in Outbreaks in the Democratic Republic of Congo between 2005 and 2012 Reveals Co-Circulation of p72 Genotypes I, IX and XIV, Including 19 Variants Mulumba–Mfumu, Leopold K. Achenbach, Jenna E. Mauldin, Matthew R. Dixon, Linda K. Tshilenge, Curé Georges Thiry, Etienne Moreno, Noelia Blanco, Esther Saegerman, Claude Lamien, Charles E. Diallo, Adama Viruses Article African swine fever (ASF) is a devastating disease of domestic pigs. It is a socioeconomically important disease, initially described from Kenya, but subsequently reported in most Sub-Saharan countries. ASF spread to Europe, South America and the Caribbean through multiple introductions which were initially eradicated—except for Sardinia—followed by re‑introduction into Europe in 2007. In this study of ASF within the Democratic Republic of the Congo, 62 domestic pig samples, collected between 2005–2012, were examined for viral DNA and sequencing at multiple loci: C-terminus of the B646L gene (p72 protein), central hypervariable region (CVR) of the B602L gene, and the E183L gene (p54 protein). Phylogenetic analyses identified three circulating genotypes: I (64.5% of samples), IX (32.3%), and XIV (3.2%). This is the first evidence of genotypes IX and XIV within this country. Examination of the CVR revealed high levels of intra-genotypic variation, with 19 identified variants. MDPI 2017-02-18 /pmc/articles/PMC5332950/ /pubmed/28218698 http://dx.doi.org/10.3390/v9020031 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mulumba–Mfumu, Leopold K.
Achenbach, Jenna E.
Mauldin, Matthew R.
Dixon, Linda K.
Tshilenge, Curé Georges
Thiry, Etienne
Moreno, Noelia
Blanco, Esther
Saegerman, Claude
Lamien, Charles E.
Diallo, Adama
Genetic Assessment of African Swine Fever Isolates Involved in Outbreaks in the Democratic Republic of Congo between 2005 and 2012 Reveals Co-Circulation of p72 Genotypes I, IX and XIV, Including 19 Variants
title Genetic Assessment of African Swine Fever Isolates Involved in Outbreaks in the Democratic Republic of Congo between 2005 and 2012 Reveals Co-Circulation of p72 Genotypes I, IX and XIV, Including 19 Variants
title_full Genetic Assessment of African Swine Fever Isolates Involved in Outbreaks in the Democratic Republic of Congo between 2005 and 2012 Reveals Co-Circulation of p72 Genotypes I, IX and XIV, Including 19 Variants
title_fullStr Genetic Assessment of African Swine Fever Isolates Involved in Outbreaks in the Democratic Republic of Congo between 2005 and 2012 Reveals Co-Circulation of p72 Genotypes I, IX and XIV, Including 19 Variants
title_full_unstemmed Genetic Assessment of African Swine Fever Isolates Involved in Outbreaks in the Democratic Republic of Congo between 2005 and 2012 Reveals Co-Circulation of p72 Genotypes I, IX and XIV, Including 19 Variants
title_short Genetic Assessment of African Swine Fever Isolates Involved in Outbreaks in the Democratic Republic of Congo between 2005 and 2012 Reveals Co-Circulation of p72 Genotypes I, IX and XIV, Including 19 Variants
title_sort genetic assessment of african swine fever isolates involved in outbreaks in the democratic republic of congo between 2005 and 2012 reveals co-circulation of p72 genotypes i, ix and xiv, including 19 variants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5332950/
https://www.ncbi.nlm.nih.gov/pubmed/28218698
http://dx.doi.org/10.3390/v9020031
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