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Oscillating Magnet Array−Based Nanomagnetic Gene Transfection: A Valuable Tool for Molecular Neurobiology Studies
To develop treatments for neurodegenerative disorders, it is critical to understand the biology and function of neurons in both normal and diseased states. Molecular studies of neurons involve the delivery of small biomolecules into cultured neurons via transfection to study genetic variants. Howeve...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5333013/ https://www.ncbi.nlm.nih.gov/pubmed/28336862 http://dx.doi.org/10.3390/nano7020028 |
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author | Subramanian, Mahendran Tyler, Aimee-Jayne Luther, Eva Maria Daniel, Elena Di Lim, Jenson Dobson, Jon |
author_facet | Subramanian, Mahendran Tyler, Aimee-Jayne Luther, Eva Maria Daniel, Elena Di Lim, Jenson Dobson, Jon |
author_sort | Subramanian, Mahendran |
collection | PubMed |
description | To develop treatments for neurodegenerative disorders, it is critical to understand the biology and function of neurons in both normal and diseased states. Molecular studies of neurons involve the delivery of small biomolecules into cultured neurons via transfection to study genetic variants. However, as cultured primary neurons are sensitive to temperature change, stress, and shifts in pH, these factors make biomolecule delivery difficult, particularly non-viral delivery. Herein we used oscillating nanomagnetic gene transfection to successfully transfect SH-SY5Y cells as well as primary hippocampal and cortical neurons on different days in vitro. This novel technique has been used to effectively deliver genetic material into various cell types, resulting in high transfection efficiency and viability. From these observations and other related studies, we suggest that oscillating nanomagnetic gene transfection is an effective method for gene delivery into hard-to-transfect neuronal cell types. |
format | Online Article Text |
id | pubmed-5333013 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-53330132017-03-21 Oscillating Magnet Array−Based Nanomagnetic Gene Transfection: A Valuable Tool for Molecular Neurobiology Studies Subramanian, Mahendran Tyler, Aimee-Jayne Luther, Eva Maria Daniel, Elena Di Lim, Jenson Dobson, Jon Nanomaterials (Basel) Brief Report To develop treatments for neurodegenerative disorders, it is critical to understand the biology and function of neurons in both normal and diseased states. Molecular studies of neurons involve the delivery of small biomolecules into cultured neurons via transfection to study genetic variants. However, as cultured primary neurons are sensitive to temperature change, stress, and shifts in pH, these factors make biomolecule delivery difficult, particularly non-viral delivery. Herein we used oscillating nanomagnetic gene transfection to successfully transfect SH-SY5Y cells as well as primary hippocampal and cortical neurons on different days in vitro. This novel technique has been used to effectively deliver genetic material into various cell types, resulting in high transfection efficiency and viability. From these observations and other related studies, we suggest that oscillating nanomagnetic gene transfection is an effective method for gene delivery into hard-to-transfect neuronal cell types. MDPI 2017-01-29 /pmc/articles/PMC5333013/ /pubmed/28336862 http://dx.doi.org/10.3390/nano7020028 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Brief Report Subramanian, Mahendran Tyler, Aimee-Jayne Luther, Eva Maria Daniel, Elena Di Lim, Jenson Dobson, Jon Oscillating Magnet Array−Based Nanomagnetic Gene Transfection: A Valuable Tool for Molecular Neurobiology Studies |
title | Oscillating Magnet Array−Based Nanomagnetic Gene Transfection: A Valuable Tool for Molecular Neurobiology Studies |
title_full | Oscillating Magnet Array−Based Nanomagnetic Gene Transfection: A Valuable Tool for Molecular Neurobiology Studies |
title_fullStr | Oscillating Magnet Array−Based Nanomagnetic Gene Transfection: A Valuable Tool for Molecular Neurobiology Studies |
title_full_unstemmed | Oscillating Magnet Array−Based Nanomagnetic Gene Transfection: A Valuable Tool for Molecular Neurobiology Studies |
title_short | Oscillating Magnet Array−Based Nanomagnetic Gene Transfection: A Valuable Tool for Molecular Neurobiology Studies |
title_sort | oscillating magnet array−based nanomagnetic gene transfection: a valuable tool for molecular neurobiology studies |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5333013/ https://www.ncbi.nlm.nih.gov/pubmed/28336862 http://dx.doi.org/10.3390/nano7020028 |
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