Hydrophobic ionic liquids for quantitative bacterial cell lysis with subsequent DNA quantification

DNA is one of the most frequently analyzed molecules in the life sciences. In this article we describe a simple and fast protocol for quantitative DNA isolation from bacteria based on hydrophobic ionic liquid supported cell lysis at elevated temperatures (120–150 °C) for subsequent PCR-based analysi...

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Detalles Bibliográficos
Autores principales: Fuchs-Telka, Sabine, Fister, Susanne, Mester, Patrick-Julian, Wagner, Martin, Rossmanith, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Paper in Forefront
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5333376/
https://www.ncbi.nlm.nih.gov/pubmed/28004172
http://dx.doi.org/10.1007/s00216-016-0112-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5333376/
https://www.ncbi.nlm.nih.gov/pubmed/28004172
http://dx.doi.org/10.1007/s00216-016-0112-x

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