Cargando…

Hydrophobic ionic liquids for quantitative bacterial cell lysis with subsequent DNA quantification

DNA is one of the most frequently analyzed molecules in the life sciences. In this article we describe a simple and fast protocol for quantitative DNA isolation from bacteria based on hydrophobic ionic liquid supported cell lysis at elevated temperatures (120–150 °C) for subsequent PCR-based analysi...

Descripción completa

Detalles Bibliográficos
Autores principales:	Fuchs-Telka, Sabine, Fister, Susanne, Mester, Patrick-Julian, Wagner, Martin, Rossmanith, Peter
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer Berlin Heidelberg 2016
Materias:	Paper in Forefront
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5333376/ https://www.ncbi.nlm.nih.gov/pubmed/28004172 http://dx.doi.org/10.1007/s00216-016-0112-x

Ejemplares similares

Evaluation of the performance of quantitative detection of the Listeria monocytogenes prfA locus with droplet digital PCR
por: Witte, Anna Kristina, et al.
Publicado: (2016)

Virucidal Influence of Ionic Liquids on Phages P100 and MS2
por: Fister, Susanne, et al.
Publicado: (2017)

Virucidal or Not Virucidal? That Is the Question—Predictability of Ionic Liquid’s Virucidal Potential in Biological Test Systems
por: Sommer, Julia, et al.
Publicado: (2018)

Quantitative determination and validation of 17 cannabinoids in cannabis and hemp using liquid chromatography-tandem mass spectrometry
por: McRae, Garnet, et al.
Publicado: (2020)

Capillary coating as an important factor in optimization of the off-line and on-line MEKC assays of the highly hydrophobic enzyme chlorophyllase
por: Nowak, Paweł Mateusz, et al.
Publicado: (2016)

Assessment of the real-time PCR and different digital PCR platforms for DNA quantification
por: Pavšič, Jernej, et al.
Publicado: (2015)

Quantification of the dynamics of population heterogeneities in CHO cultures with stably integrated fluorescent markers
por: Möller, Johannes, et al.
Publicado: (2020)

Site-specific carbon isotope measurements of vanillin reference materials by nuclear magnetic resonance spectrometry
por: Le, Phuong Mai, et al.
Publicado: (2022)

Interlaboratory assessment of quantification of SARS-CoV-2 RNA by reverse transcription digital PCR
por: Niu, Chunyan, et al.
Publicado: (2021)

Towards standardisation of cell-free DNA measurement in plasma: controls for extraction efficiency, fragment size bias and quantification
por: Devonshire, Alison S., et al.
Publicado: (2014)

Comparing (13)C methyl and deuterated methyl isotopic labeling for the quantification of methyl cellulose patterns using mass spectrometry
por: Schleicher, Sarah, et al.
Publicado: (2023)

Determination of porphyrins in oral bacteria by liquid chromatography electrospray ionization tandem mass spectrometry
por: Fyrestam, Jonas, et al.
Publicado: (2015)

Isothermal circular strand displacement–based assay for microRNA detection in liquid biopsy
por: Bellassai, Noemi, et al.
Publicado: (2022)

An antibody-free LC-MS/MS method for the quantification of intact insulin-like growth factors 1 and 2 in human plasma
por: Pratt, Mark S., et al.
Publicado: (2021)

Bacterial Resistance Toward Antimicrobial Ionic Liquids Mediated by Multidrug Efflux Pumps
por: Gundolf, Tobias, et al.
Publicado: (2022)

Liquid chromatography–high-resolution tandem mass spectrometry of anatoxins, including new conjugates and reduction products
por: Beach, Daniel G., et al.
Publicado: (2023)

Liquid chromatographic nanofractionation with parallel mass spectrometric detection for the screening of plasmin inhibitors and (metallo)proteinases in snake venoms
por: Zietek, Barbara M., et al.
Publicado: (2018)

N-Alkylpyridinium sulfonates for retention time indexing in reversed-phase-liquid chromatography-mass spectrometry–based metabolomics
por: Stoffel, Rainer, et al.
Publicado: (2021)

First bioelectronic immunoplatform for quantitative secretomic analysis of total and metastasis-driven glycosylated haptoglobin
por: Muñoz-San Martín, Cristina, et al.
Publicado: (2022)

Affinity profiling of monoclonal antibody and antibody-drug-conjugate preparations by coupled liquid chromatography-surface plasmon resonance biosensing
por: Lakayan, Dina, et al.
Publicado: (2018)

Development of a sandwich ELISA for the specific quantitation of hemagglutinin (HA)-tagged proteins during their inducible expression in Escherichia coli
por: Yin, Zihan, et al.
Publicado: (2023)

Portable, quantitative, and sequential monitoring of copper ions and pyrophosphate based on a DNAzyme-Fe(3)O(4) nanosystem and glucometer readout
por: Gu, Chunchuan, et al.
Publicado: (2021)

Multi-residue ultra-performance liquid chromatography coupled with tandem mass spectrometry method for comprehensive multi-class anthropogenic compounds of emerging concern analysis in a catchment-based exposure-driven study
por: Proctor, Kathryn, et al.
Publicado: (2019)

Impact of instrumental settings in electrospray ionization ion trap mass spectrometry on the analysis of multi-CH(3)-/CD(3)-isotopologs in cellulose ether analysis: a quantitative evaluation
por: Schleicher, Sarah, et al.
Publicado: (2021)

Quantitation of methotrexate polyglutamates in human whole blood, erythrocytes and leukocytes collected via venepuncture and volumetric absorptive micro-sampling: a green LC–MS/MS-based method
por: Daraghmeh, Dala N., et al.
Publicado: (2022)

Antimicrobial and Virucidal Potential of Morpholinium-Based Ionic Liquids
por: Michalski, Jakub, et al.
Publicado: (2023)

Characterization of the specific interaction between the DNA aptamer sgc8c and protein tyrosine kinase-7 receptors at the surface of T-cells by biosensing AFM
por: Leitner, Michael, et al.
Publicado: (2017)

The influence of the shape of Au nanoparticles on the catalytic current of fructose dehydrogenase
por: Bollella, Paolo, et al.
Publicado: (2019)

Comparison of Whiskbroom and Pushbroom darkfield elastic light scattering spectroscopic imaging for head and neck cancer identification in a mouse model
por: Bassler, Miriam C., et al.
Publicado: (2021)

Liposome-based high-throughput and point-of-care assays toward the quick, simple, and sensitive detection of neutralizing antibodies against SARS-CoV-2 in patient sera
por: Streif, Simon, et al.
Publicado: (2023)

Suspect screening and targeted analysis of acyl coenzyme A thioesters in bacterial cultures using a high-resolution tribrid mass spectrometer
por: Cakić, Nevenka, et al.
Publicado: (2021)

PCR-Stop analysis as a new tool for qPCR assay validation
por: Witte, Anna Kristina, et al.
Publicado: (2018)

Induction of the viable but non-culturable state in bacterial pathogens by household cleaners and inorganic salts
por: Robben, Christian, et al.
Publicado: (2018)

Detailed structural elucidation of polyesters and acrylates using Fourier transform mass spectrometry
por: Simonsick, William J., et al.
Publicado: (2008)

An on-line post-column detection system for the detection of reactive-oxygen-species-producing compounds and antioxidants in mixtures
por: Kool, Jeroen, et al.
Publicado: (2007)

Stable isotope dilution assay for the accurate determination of mycotoxins in maize by UHPLC-MS/MS
por: Varga, Elisabeth, et al.
Publicado: (2012)

Development of a multiplex DNA-based traceability tool for crop plant materials
por: Voorhuijzen, Marleen M., et al.
Publicado: (2011)

NMR methods to monitor the enzymatic depolymerization of heparin
por: Limtiaco, John F. K., et al.
Publicado: (2010)

Masking effect of anti-androgens on androgenic activity in European river sediment unveiled by effect-directed analysis
por: Weiss, Jana M., et al.
Publicado: (2009)

N-Methyl-4-hydrazino-7-nitrobenzofurazan: a fluorogenic substrate for peroxidase-like DNAzyme, and its potential application
por: Kosman, Joanna, et al.
Publicado: (2014)

Cannot write session to /tmp/vufind_sessions/sess_ck0admtpsl44otg516g2dj11ie