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Automated sub-5 nm image registration in integrated correlative fluorescence and electron microscopy using cathodoluminescence pointers
In the biological sciences, data from fluorescence and electron microscopy is correlated to allow fluorescence biomolecule identification within the cellular ultrastructure and/or ultrastructural analysis following live-cell imaging. High-accuracy (sub-100 nm) image overlay requires the addition of...
Autores principales: | Haring, Martijn T., Liv, Nalan, Zonnevylle, A. Christiaan, Narvaez, Angela C., Voortman, Lenard M., Kruit, Pieter, Hoogenboom, Jacob P. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5333625/ https://www.ncbi.nlm.nih.gov/pubmed/28252673 http://dx.doi.org/10.1038/srep43621 |
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