Cargando…

Stromal interaction molecule 1 regulates growth, cell cycle, and apoptosis of human tongue squamous carcinoma cells

Oral tongue squamous cell carcinoma (OTSCC) is the most common type of oral carcinomas. However, the molecular mechanism by which OTSCC developed is not fully identified. Stromal interaction molecule 1 (STIM1) is a transmembrane protein, mainly located in the endoplasmic reticulum (ER). STIM1 is inv...

Descripción completa

Detalles Bibliográficos
Autores principales: Cui, Xiaobo, Song, Laixiao, Bai, Yunfei, Wang, Yaping, Wang, Boqian, Wang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5333779/
https://www.ncbi.nlm.nih.gov/pubmed/28108672
http://dx.doi.org/10.1042/BSR20160519
_version_ 1782511768861409280
author Cui, Xiaobo
Song, Laixiao
Bai, Yunfei
Wang, Yaping
Wang, Boqian
Wang, Wei
author_facet Cui, Xiaobo
Song, Laixiao
Bai, Yunfei
Wang, Yaping
Wang, Boqian
Wang, Wei
author_sort Cui, Xiaobo
collection PubMed
description Oral tongue squamous cell carcinoma (OTSCC) is the most common type of oral carcinomas. However, the molecular mechanism by which OTSCC developed is not fully identified. Stromal interaction molecule 1 (STIM1) is a transmembrane protein, mainly located in the endoplasmic reticulum (ER). STIM1 is involved in several types of cancers. Here, we report that STIM1 contributes to the development of human OTSCC. We knocked down STIM1 in OTSCC cell line Tca-8113 with lentivirus-mediated shRNA and found that STIM1 knockdown repressed the proliferation of Tca-8113 cells. In addition, we also showed that STIM1 deficiency reduced colony number of Tca-8113 cells. Knockdown of STIM1 repressed cells to enter M phase of cell cycle and induced cellular apoptosis. Furthermore, we performed microarray and bioinformatics analysis and found that STIM1 was associated with p53 and MAPK pathways, which may contribute to the effects of STIM1 on cell growth, cell cycle, and apoptosis. Finally, we confirmed that STIM1 controlled the expression of MDM2, cyclin-dependent kinase 4 (CDK4), and growth arrest and DNA damage inducible α (GADD45A) in OTSCC cells. In conclusion, we provide evidence that STIM1 contributes to the development of OTSCC partially through regulating p53 and MAPK pathways to promote cell cycle and survival.
format Online
Article
Text
id pubmed-5333779
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Portland Press Ltd.
record_format MEDLINE/PubMed
spelling pubmed-53337792017-04-30 Stromal interaction molecule 1 regulates growth, cell cycle, and apoptosis of human tongue squamous carcinoma cells Cui, Xiaobo Song, Laixiao Bai, Yunfei Wang, Yaping Wang, Boqian Wang, Wei Biosci Rep Research Articles Oral tongue squamous cell carcinoma (OTSCC) is the most common type of oral carcinomas. However, the molecular mechanism by which OTSCC developed is not fully identified. Stromal interaction molecule 1 (STIM1) is a transmembrane protein, mainly located in the endoplasmic reticulum (ER). STIM1 is involved in several types of cancers. Here, we report that STIM1 contributes to the development of human OTSCC. We knocked down STIM1 in OTSCC cell line Tca-8113 with lentivirus-mediated shRNA and found that STIM1 knockdown repressed the proliferation of Tca-8113 cells. In addition, we also showed that STIM1 deficiency reduced colony number of Tca-8113 cells. Knockdown of STIM1 repressed cells to enter M phase of cell cycle and induced cellular apoptosis. Furthermore, we performed microarray and bioinformatics analysis and found that STIM1 was associated with p53 and MAPK pathways, which may contribute to the effects of STIM1 on cell growth, cell cycle, and apoptosis. Finally, we confirmed that STIM1 controlled the expression of MDM2, cyclin-dependent kinase 4 (CDK4), and growth arrest and DNA damage inducible α (GADD45A) in OTSCC cells. In conclusion, we provide evidence that STIM1 contributes to the development of OTSCC partially through regulating p53 and MAPK pathways to promote cell cycle and survival. Portland Press Ltd. 2017-03-02 /pmc/articles/PMC5333779/ /pubmed/28108672 http://dx.doi.org/10.1042/BSR20160519 Text en © 2017 The Author(s) http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Cui, Xiaobo
Song, Laixiao
Bai, Yunfei
Wang, Yaping
Wang, Boqian
Wang, Wei
Stromal interaction molecule 1 regulates growth, cell cycle, and apoptosis of human tongue squamous carcinoma cells
title Stromal interaction molecule 1 regulates growth, cell cycle, and apoptosis of human tongue squamous carcinoma cells
title_full Stromal interaction molecule 1 regulates growth, cell cycle, and apoptosis of human tongue squamous carcinoma cells
title_fullStr Stromal interaction molecule 1 regulates growth, cell cycle, and apoptosis of human tongue squamous carcinoma cells
title_full_unstemmed Stromal interaction molecule 1 regulates growth, cell cycle, and apoptosis of human tongue squamous carcinoma cells
title_short Stromal interaction molecule 1 regulates growth, cell cycle, and apoptosis of human tongue squamous carcinoma cells
title_sort stromal interaction molecule 1 regulates growth, cell cycle, and apoptosis of human tongue squamous carcinoma cells
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5333779/
https://www.ncbi.nlm.nih.gov/pubmed/28108672
http://dx.doi.org/10.1042/BSR20160519
work_keys_str_mv AT cuixiaobo stromalinteractionmolecule1regulatesgrowthcellcycleandapoptosisofhumantonguesquamouscarcinomacells
AT songlaixiao stromalinteractionmolecule1regulatesgrowthcellcycleandapoptosisofhumantonguesquamouscarcinomacells
AT baiyunfei stromalinteractionmolecule1regulatesgrowthcellcycleandapoptosisofhumantonguesquamouscarcinomacells
AT wangyaping stromalinteractionmolecule1regulatesgrowthcellcycleandapoptosisofhumantonguesquamouscarcinomacells
AT wangboqian stromalinteractionmolecule1regulatesgrowthcellcycleandapoptosisofhumantonguesquamouscarcinomacells
AT wangwei stromalinteractionmolecule1regulatesgrowthcellcycleandapoptosisofhumantonguesquamouscarcinomacells