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Herbivore-induced chemical and molecular responses of the kelps Laminaria digitata and Lessonia spicata

Kelps are founding species of temperate marine ecosystems, living in intertidal coastal areas where they are often challenged by generalist and specialist herbivores. As most sessile organisms, kelps develop defensive strategies to restrain grazing damage and preserve their own fitness during intera...

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Autores principales: Ritter, Andrés, Cabioch, Léa, Brillet-Guéguen, Loraine, Corre, Erwan, Cosse, Audrey, Dartevelle, Laurence, Duruflé, Harold, Fasshauer, Carina, Goulitquer, Sophie, Thomas, François, Correa, Juan A., Potin, Philippe, Faugeron, Sylvain, Leblanc, Catherine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5333891/
https://www.ncbi.nlm.nih.gov/pubmed/28253346
http://dx.doi.org/10.1371/journal.pone.0173315
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author Ritter, Andrés
Cabioch, Léa
Brillet-Guéguen, Loraine
Corre, Erwan
Cosse, Audrey
Dartevelle, Laurence
Duruflé, Harold
Fasshauer, Carina
Goulitquer, Sophie
Thomas, François
Correa, Juan A.
Potin, Philippe
Faugeron, Sylvain
Leblanc, Catherine
author_facet Ritter, Andrés
Cabioch, Léa
Brillet-Guéguen, Loraine
Corre, Erwan
Cosse, Audrey
Dartevelle, Laurence
Duruflé, Harold
Fasshauer, Carina
Goulitquer, Sophie
Thomas, François
Correa, Juan A.
Potin, Philippe
Faugeron, Sylvain
Leblanc, Catherine
author_sort Ritter, Andrés
collection PubMed
description Kelps are founding species of temperate marine ecosystems, living in intertidal coastal areas where they are often challenged by generalist and specialist herbivores. As most sessile organisms, kelps develop defensive strategies to restrain grazing damage and preserve their own fitness during interactions with herbivores. To decipher some inducible defense and signaling mechanisms, we carried out metabolome and transcriptome analyses in two emblematic kelp species, Lessonia spicata from South Pacific coasts and Laminaria digitata from North Atlantic, when challenged with their main specialist herbivores. Mass spectrometry based metabolomics revealed large metabolic changes induced in these two brown algae following challenges with their own specialist herbivores. Targeted metabolic profiling of L. spicata further showed that free fatty acid (FFA) and amino acid (AA) metabolisms were particularly regulated under grazing. An early stress response was illustrated by the accumulation of Sulphur containing amino acids in the first twelve hours of herbivory pressure. At latter time periods (after 24 hours), we observed FFA liberation and eicosanoid oxylipins synthesis likely representing metabolites related to stress. Global transcriptomic analysis identified sets of candidate genes specifically induced by grazing in both kelps. qPCR analysis of the top candidate genes during a 48-hours time course validated the results. Most of these genes were particularly activated by herbivore challenge after 24 hours, suggesting that transcriptional reprogramming could be operated at this time period. We demonstrated the potential utility of these genes as molecular markers for herbivory by measuring their inductions in grazed individuals of field harvested L. digitata and L. spicata. By unravelling the regulation of some metabolites and genes following grazing pressure in two kelps representative of the two hemispheres, this work contributes to provide a set of herbivore-induced chemical and molecular responses in kelp species, showing similar inducible responses upon specialist herbivores in their respective ecosystems.
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spelling pubmed-53338912017-03-10 Herbivore-induced chemical and molecular responses of the kelps Laminaria digitata and Lessonia spicata Ritter, Andrés Cabioch, Léa Brillet-Guéguen, Loraine Corre, Erwan Cosse, Audrey Dartevelle, Laurence Duruflé, Harold Fasshauer, Carina Goulitquer, Sophie Thomas, François Correa, Juan A. Potin, Philippe Faugeron, Sylvain Leblanc, Catherine PLoS One Research Article Kelps are founding species of temperate marine ecosystems, living in intertidal coastal areas where they are often challenged by generalist and specialist herbivores. As most sessile organisms, kelps develop defensive strategies to restrain grazing damage and preserve their own fitness during interactions with herbivores. To decipher some inducible defense and signaling mechanisms, we carried out metabolome and transcriptome analyses in two emblematic kelp species, Lessonia spicata from South Pacific coasts and Laminaria digitata from North Atlantic, when challenged with their main specialist herbivores. Mass spectrometry based metabolomics revealed large metabolic changes induced in these two brown algae following challenges with their own specialist herbivores. Targeted metabolic profiling of L. spicata further showed that free fatty acid (FFA) and amino acid (AA) metabolisms were particularly regulated under grazing. An early stress response was illustrated by the accumulation of Sulphur containing amino acids in the first twelve hours of herbivory pressure. At latter time periods (after 24 hours), we observed FFA liberation and eicosanoid oxylipins synthesis likely representing metabolites related to stress. Global transcriptomic analysis identified sets of candidate genes specifically induced by grazing in both kelps. qPCR analysis of the top candidate genes during a 48-hours time course validated the results. Most of these genes were particularly activated by herbivore challenge after 24 hours, suggesting that transcriptional reprogramming could be operated at this time period. We demonstrated the potential utility of these genes as molecular markers for herbivory by measuring their inductions in grazed individuals of field harvested L. digitata and L. spicata. By unravelling the regulation of some metabolites and genes following grazing pressure in two kelps representative of the two hemispheres, this work contributes to provide a set of herbivore-induced chemical and molecular responses in kelp species, showing similar inducible responses upon specialist herbivores in their respective ecosystems. Public Library of Science 2017-03-02 /pmc/articles/PMC5333891/ /pubmed/28253346 http://dx.doi.org/10.1371/journal.pone.0173315 Text en © 2017 Ritter et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ritter, Andrés
Cabioch, Léa
Brillet-Guéguen, Loraine
Corre, Erwan
Cosse, Audrey
Dartevelle, Laurence
Duruflé, Harold
Fasshauer, Carina
Goulitquer, Sophie
Thomas, François
Correa, Juan A.
Potin, Philippe
Faugeron, Sylvain
Leblanc, Catherine
Herbivore-induced chemical and molecular responses of the kelps Laminaria digitata and Lessonia spicata
title Herbivore-induced chemical and molecular responses of the kelps Laminaria digitata and Lessonia spicata
title_full Herbivore-induced chemical and molecular responses of the kelps Laminaria digitata and Lessonia spicata
title_fullStr Herbivore-induced chemical and molecular responses of the kelps Laminaria digitata and Lessonia spicata
title_full_unstemmed Herbivore-induced chemical and molecular responses of the kelps Laminaria digitata and Lessonia spicata
title_short Herbivore-induced chemical and molecular responses of the kelps Laminaria digitata and Lessonia spicata
title_sort herbivore-induced chemical and molecular responses of the kelps laminaria digitata and lessonia spicata
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5333891/
https://www.ncbi.nlm.nih.gov/pubmed/28253346
http://dx.doi.org/10.1371/journal.pone.0173315
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