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Cauda Epididymis-Specific Beta-Defensin 126 Promotes Sperm Motility but Not Fertilizing Ability in Cattle
Bovine beta-defensin 126 (BBD126) exhibits preferential expression for the cauda epididymis of males, where it is absorbed onto the tail and postacrosomal region of the sperm. The aim of this study was to examine the role of BBD126 in bull sperm function. Fresh and frozen-thawed semen were incubated...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society for the Study of Reproduction, Inc.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5333942/ https://www.ncbi.nlm.nih.gov/pubmed/27707713 http://dx.doi.org/10.1095/biolreprod.116.138792 |
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author | Fernandez-Fuertes, Beatriz Narciandi, Fernando O'Farrelly, Cliona Kelly, Alan K. Fair, Sean Meade, Kieran G. Lonergan, Patrick |
author_facet | Fernandez-Fuertes, Beatriz Narciandi, Fernando O'Farrelly, Cliona Kelly, Alan K. Fair, Sean Meade, Kieran G. Lonergan, Patrick |
author_sort | Fernandez-Fuertes, Beatriz |
collection | PubMed |
description | Bovine beta-defensin 126 (BBD126) exhibits preferential expression for the cauda epididymis of males, where it is absorbed onto the tail and postacrosomal region of the sperm. The aim of this study was to examine the role of BBD126 in bull sperm function. Fresh and frozen-thawed semen were incubated in the presence of different capacitating agents as well as with phosphatidylinositol-specific phospholipase C. These treatments, which have been successful in releasing beta-defensin 126 from macaque sperm, proved to be ineffective in bull sperm. This finding suggests that the protein behaves in a different manner in the bovine. The lack of success in removing BBD126 led us to use corpus epididymis sperm, a model in which the protein is not present, to study its functional role. Corpus sperm were incubated with cauda epididymal fluid (CEF) in the absence or presence of BBD126 antibody or with recombinant BBD126 (rBBD126). Confocal microscopy revealed that rBBD126 binds to corpus sperm with the same pattern observed for BBD126 in cauda sperm, whereas an aberrant binding pattern is observed when sperm are subject to CEF incubation. Addition of CEF increased motility as well as the number of corpus sperm migrating through cervical mucus from estrus cows. However, it decreased the ability of sperm to fertilize in vitro matured oocytes. The presence of the antibody failed to abrogate these effects. Furthermore, when rBBD126 was added in the absence of other factors and proteins from the CEF, an increase in motility was also observed and no negative effects in fertility were seen. These results suggest that BBD126 plays a key role in the acquisition of sperm motility in the epididymis. |
format | Online Article Text |
id | pubmed-5333942 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Society for the Study of Reproduction, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-53339422017-12-01 Cauda Epididymis-Specific Beta-Defensin 126 Promotes Sperm Motility but Not Fertilizing Ability in Cattle Fernandez-Fuertes, Beatriz Narciandi, Fernando O'Farrelly, Cliona Kelly, Alan K. Fair, Sean Meade, Kieran G. Lonergan, Patrick Biol Reprod Articles Bovine beta-defensin 126 (BBD126) exhibits preferential expression for the cauda epididymis of males, where it is absorbed onto the tail and postacrosomal region of the sperm. The aim of this study was to examine the role of BBD126 in bull sperm function. Fresh and frozen-thawed semen were incubated in the presence of different capacitating agents as well as with phosphatidylinositol-specific phospholipase C. These treatments, which have been successful in releasing beta-defensin 126 from macaque sperm, proved to be ineffective in bull sperm. This finding suggests that the protein behaves in a different manner in the bovine. The lack of success in removing BBD126 led us to use corpus epididymis sperm, a model in which the protein is not present, to study its functional role. Corpus sperm were incubated with cauda epididymal fluid (CEF) in the absence or presence of BBD126 antibody or with recombinant BBD126 (rBBD126). Confocal microscopy revealed that rBBD126 binds to corpus sperm with the same pattern observed for BBD126 in cauda sperm, whereas an aberrant binding pattern is observed when sperm are subject to CEF incubation. Addition of CEF increased motility as well as the number of corpus sperm migrating through cervical mucus from estrus cows. However, it decreased the ability of sperm to fertilize in vitro matured oocytes. The presence of the antibody failed to abrogate these effects. Furthermore, when rBBD126 was added in the absence of other factors and proteins from the CEF, an increase in motility was also observed and no negative effects in fertility were seen. These results suggest that BBD126 plays a key role in the acquisition of sperm motility in the epididymis. Society for the Study of Reproduction, Inc. 2016-10-05 2016-12 /pmc/articles/PMC5333942/ /pubmed/27707713 http://dx.doi.org/10.1095/biolreprod.116.138792 Text en © 2016 by the Society for the Study of Reproduction, Inc. http://creativecommons.org/licenses/by-nc/4.0/ This article is available under a Creative Commons License 4.0 (Attribution-Non-Commercial), as described at http://creativecommons.org/licenses/by-nc/4.0 |
spellingShingle | Articles Fernandez-Fuertes, Beatriz Narciandi, Fernando O'Farrelly, Cliona Kelly, Alan K. Fair, Sean Meade, Kieran G. Lonergan, Patrick Cauda Epididymis-Specific Beta-Defensin 126 Promotes Sperm Motility but Not Fertilizing Ability in Cattle |
title | Cauda Epididymis-Specific Beta-Defensin 126 Promotes Sperm Motility but Not Fertilizing Ability in Cattle |
title_full | Cauda Epididymis-Specific Beta-Defensin 126 Promotes Sperm Motility but Not Fertilizing Ability in Cattle |
title_fullStr | Cauda Epididymis-Specific Beta-Defensin 126 Promotes Sperm Motility but Not Fertilizing Ability in Cattle |
title_full_unstemmed | Cauda Epididymis-Specific Beta-Defensin 126 Promotes Sperm Motility but Not Fertilizing Ability in Cattle |
title_short | Cauda Epididymis-Specific Beta-Defensin 126 Promotes Sperm Motility but Not Fertilizing Ability in Cattle |
title_sort | cauda epididymis-specific beta-defensin 126 promotes sperm motility but not fertilizing ability in cattle |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5333942/ https://www.ncbi.nlm.nih.gov/pubmed/27707713 http://dx.doi.org/10.1095/biolreprod.116.138792 |
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