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A Comprehensive Phenotypic Investigation of the “Pod-Shattering Syndrome” in Common Bean

Seed shattering in crops is a key domestication trait due to its relevance for seed dispersal, yield, and fundamental questions in evolution (e.g., convergent evolution). Here, we focused on pod shattering in common bean (Phaseolus vulgaris L.), the most important legume crop for human consuption in...

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Autores principales: Murgia, Maria L., Attene, Giovanna, Rodriguez, Monica, Bitocchi, Elena, Bellucci, Elisa, Fois, Davide, Nanni, Laura, Gioia, Tania, Albani, Diego M., Papa, Roberto, Rau, Domenico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5334323/
https://www.ncbi.nlm.nih.gov/pubmed/28316606
http://dx.doi.org/10.3389/fpls.2017.00251
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author Murgia, Maria L.
Attene, Giovanna
Rodriguez, Monica
Bitocchi, Elena
Bellucci, Elisa
Fois, Davide
Nanni, Laura
Gioia, Tania
Albani, Diego M.
Papa, Roberto
Rau, Domenico
author_facet Murgia, Maria L.
Attene, Giovanna
Rodriguez, Monica
Bitocchi, Elena
Bellucci, Elisa
Fois, Davide
Nanni, Laura
Gioia, Tania
Albani, Diego M.
Papa, Roberto
Rau, Domenico
author_sort Murgia, Maria L.
collection PubMed
description Seed shattering in crops is a key domestication trait due to its relevance for seed dispersal, yield, and fundamental questions in evolution (e.g., convergent evolution). Here, we focused on pod shattering in common bean (Phaseolus vulgaris L.), the most important legume crop for human consuption in the world. With this main aim, we developed a methodological pipeline that comprises a thorough characterization under field conditions, including also the chemical composition and histological analysis of the pod valves. The pipeline was developed based on the assumption that the shattering trait itself can be treated in principle as a “syndrome” (i.e., a set of correlated different traits) at the pod level. We characterized a population of 267 introgression lines that were developed ad-hoc to study shattering in common bean. Three main objectives were sought: (1) to dissect the shattering trait into its “components,” of level (percentage of shattering pods per plant) and mode (percentage of pods with twisting or non-twisting valves); (2) to test whether shattering is associated to the chemical composition and/or the histological characteristics of the pod valves; and (3) to test the associations between shattering and other plant traits. We can conclude the following: Very high shattering levels can be achieved in different modes; shattering resistance is mainly a qualitative trait; and high shattering levels is correlated with high carbon and lignin contents of the pod valves and with specific histological charaterstics of the ventral sheath and the inner fibrous layer of the pod wall. Our data also suggest that shattering comes with a “cost,” as it is associated with low pod size, low seed weight per pod, high pod weight, and low seed to pod-valves ratio; indeed, it can be more exaustively described as a syndrome at the pod level. Our work suggests that the valve chemical composition (i.e., carbon and lignin content) can be used for a high troughput phenotyping procedures for shattering phenotyping. Finally, we believe that the application of our pipeline will greatly facilitate comparative studies among legume crops, and gene tagging.
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spelling pubmed-53343232017-03-17 A Comprehensive Phenotypic Investigation of the “Pod-Shattering Syndrome” in Common Bean Murgia, Maria L. Attene, Giovanna Rodriguez, Monica Bitocchi, Elena Bellucci, Elisa Fois, Davide Nanni, Laura Gioia, Tania Albani, Diego M. Papa, Roberto Rau, Domenico Front Plant Sci Plant Science Seed shattering in crops is a key domestication trait due to its relevance for seed dispersal, yield, and fundamental questions in evolution (e.g., convergent evolution). Here, we focused on pod shattering in common bean (Phaseolus vulgaris L.), the most important legume crop for human consuption in the world. With this main aim, we developed a methodological pipeline that comprises a thorough characterization under field conditions, including also the chemical composition and histological analysis of the pod valves. The pipeline was developed based on the assumption that the shattering trait itself can be treated in principle as a “syndrome” (i.e., a set of correlated different traits) at the pod level. We characterized a population of 267 introgression lines that were developed ad-hoc to study shattering in common bean. Three main objectives were sought: (1) to dissect the shattering trait into its “components,” of level (percentage of shattering pods per plant) and mode (percentage of pods with twisting or non-twisting valves); (2) to test whether shattering is associated to the chemical composition and/or the histological characteristics of the pod valves; and (3) to test the associations between shattering and other plant traits. We can conclude the following: Very high shattering levels can be achieved in different modes; shattering resistance is mainly a qualitative trait; and high shattering levels is correlated with high carbon and lignin contents of the pod valves and with specific histological charaterstics of the ventral sheath and the inner fibrous layer of the pod wall. Our data also suggest that shattering comes with a “cost,” as it is associated with low pod size, low seed weight per pod, high pod weight, and low seed to pod-valves ratio; indeed, it can be more exaustively described as a syndrome at the pod level. Our work suggests that the valve chemical composition (i.e., carbon and lignin content) can be used for a high troughput phenotyping procedures for shattering phenotyping. Finally, we believe that the application of our pipeline will greatly facilitate comparative studies among legume crops, and gene tagging. Frontiers Media S.A. 2017-03-03 /pmc/articles/PMC5334323/ /pubmed/28316606 http://dx.doi.org/10.3389/fpls.2017.00251 Text en Copyright © 2017 Murgia, Attene, Rodriguez, Bitocchi, Bellucci, Fois, Nanni, Gioia, Albani, Papa and Rau. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Murgia, Maria L.
Attene, Giovanna
Rodriguez, Monica
Bitocchi, Elena
Bellucci, Elisa
Fois, Davide
Nanni, Laura
Gioia, Tania
Albani, Diego M.
Papa, Roberto
Rau, Domenico
A Comprehensive Phenotypic Investigation of the “Pod-Shattering Syndrome” in Common Bean
title A Comprehensive Phenotypic Investigation of the “Pod-Shattering Syndrome” in Common Bean
title_full A Comprehensive Phenotypic Investigation of the “Pod-Shattering Syndrome” in Common Bean
title_fullStr A Comprehensive Phenotypic Investigation of the “Pod-Shattering Syndrome” in Common Bean
title_full_unstemmed A Comprehensive Phenotypic Investigation of the “Pod-Shattering Syndrome” in Common Bean
title_short A Comprehensive Phenotypic Investigation of the “Pod-Shattering Syndrome” in Common Bean
title_sort comprehensive phenotypic investigation of the “pod-shattering syndrome” in common bean
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5334323/
https://www.ncbi.nlm.nih.gov/pubmed/28316606
http://dx.doi.org/10.3389/fpls.2017.00251
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