Cargando…
A Comprehensive Phenotypic Investigation of the “Pod-Shattering Syndrome” in Common Bean
Seed shattering in crops is a key domestication trait due to its relevance for seed dispersal, yield, and fundamental questions in evolution (e.g., convergent evolution). Here, we focused on pod shattering in common bean (Phaseolus vulgaris L.), the most important legume crop for human consuption in...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5334323/ https://www.ncbi.nlm.nih.gov/pubmed/28316606 http://dx.doi.org/10.3389/fpls.2017.00251 |
_version_ | 1782511831248535552 |
---|---|
author | Murgia, Maria L. Attene, Giovanna Rodriguez, Monica Bitocchi, Elena Bellucci, Elisa Fois, Davide Nanni, Laura Gioia, Tania Albani, Diego M. Papa, Roberto Rau, Domenico |
author_facet | Murgia, Maria L. Attene, Giovanna Rodriguez, Monica Bitocchi, Elena Bellucci, Elisa Fois, Davide Nanni, Laura Gioia, Tania Albani, Diego M. Papa, Roberto Rau, Domenico |
author_sort | Murgia, Maria L. |
collection | PubMed |
description | Seed shattering in crops is a key domestication trait due to its relevance for seed dispersal, yield, and fundamental questions in evolution (e.g., convergent evolution). Here, we focused on pod shattering in common bean (Phaseolus vulgaris L.), the most important legume crop for human consuption in the world. With this main aim, we developed a methodological pipeline that comprises a thorough characterization under field conditions, including also the chemical composition and histological analysis of the pod valves. The pipeline was developed based on the assumption that the shattering trait itself can be treated in principle as a “syndrome” (i.e., a set of correlated different traits) at the pod level. We characterized a population of 267 introgression lines that were developed ad-hoc to study shattering in common bean. Three main objectives were sought: (1) to dissect the shattering trait into its “components,” of level (percentage of shattering pods per plant) and mode (percentage of pods with twisting or non-twisting valves); (2) to test whether shattering is associated to the chemical composition and/or the histological characteristics of the pod valves; and (3) to test the associations between shattering and other plant traits. We can conclude the following: Very high shattering levels can be achieved in different modes; shattering resistance is mainly a qualitative trait; and high shattering levels is correlated with high carbon and lignin contents of the pod valves and with specific histological charaterstics of the ventral sheath and the inner fibrous layer of the pod wall. Our data also suggest that shattering comes with a “cost,” as it is associated with low pod size, low seed weight per pod, high pod weight, and low seed to pod-valves ratio; indeed, it can be more exaustively described as a syndrome at the pod level. Our work suggests that the valve chemical composition (i.e., carbon and lignin content) can be used for a high troughput phenotyping procedures for shattering phenotyping. Finally, we believe that the application of our pipeline will greatly facilitate comparative studies among legume crops, and gene tagging. |
format | Online Article Text |
id | pubmed-5334323 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-53343232017-03-17 A Comprehensive Phenotypic Investigation of the “Pod-Shattering Syndrome” in Common Bean Murgia, Maria L. Attene, Giovanna Rodriguez, Monica Bitocchi, Elena Bellucci, Elisa Fois, Davide Nanni, Laura Gioia, Tania Albani, Diego M. Papa, Roberto Rau, Domenico Front Plant Sci Plant Science Seed shattering in crops is a key domestication trait due to its relevance for seed dispersal, yield, and fundamental questions in evolution (e.g., convergent evolution). Here, we focused on pod shattering in common bean (Phaseolus vulgaris L.), the most important legume crop for human consuption in the world. With this main aim, we developed a methodological pipeline that comprises a thorough characterization under field conditions, including also the chemical composition and histological analysis of the pod valves. The pipeline was developed based on the assumption that the shattering trait itself can be treated in principle as a “syndrome” (i.e., a set of correlated different traits) at the pod level. We characterized a population of 267 introgression lines that were developed ad-hoc to study shattering in common bean. Three main objectives were sought: (1) to dissect the shattering trait into its “components,” of level (percentage of shattering pods per plant) and mode (percentage of pods with twisting or non-twisting valves); (2) to test whether shattering is associated to the chemical composition and/or the histological characteristics of the pod valves; and (3) to test the associations between shattering and other plant traits. We can conclude the following: Very high shattering levels can be achieved in different modes; shattering resistance is mainly a qualitative trait; and high shattering levels is correlated with high carbon and lignin contents of the pod valves and with specific histological charaterstics of the ventral sheath and the inner fibrous layer of the pod wall. Our data also suggest that shattering comes with a “cost,” as it is associated with low pod size, low seed weight per pod, high pod weight, and low seed to pod-valves ratio; indeed, it can be more exaustively described as a syndrome at the pod level. Our work suggests that the valve chemical composition (i.e., carbon and lignin content) can be used for a high troughput phenotyping procedures for shattering phenotyping. Finally, we believe that the application of our pipeline will greatly facilitate comparative studies among legume crops, and gene tagging. Frontiers Media S.A. 2017-03-03 /pmc/articles/PMC5334323/ /pubmed/28316606 http://dx.doi.org/10.3389/fpls.2017.00251 Text en Copyright © 2017 Murgia, Attene, Rodriguez, Bitocchi, Bellucci, Fois, Nanni, Gioia, Albani, Papa and Rau. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Murgia, Maria L. Attene, Giovanna Rodriguez, Monica Bitocchi, Elena Bellucci, Elisa Fois, Davide Nanni, Laura Gioia, Tania Albani, Diego M. Papa, Roberto Rau, Domenico A Comprehensive Phenotypic Investigation of the “Pod-Shattering Syndrome” in Common Bean |
title | A Comprehensive Phenotypic Investigation of the “Pod-Shattering Syndrome” in Common Bean |
title_full | A Comprehensive Phenotypic Investigation of the “Pod-Shattering Syndrome” in Common Bean |
title_fullStr | A Comprehensive Phenotypic Investigation of the “Pod-Shattering Syndrome” in Common Bean |
title_full_unstemmed | A Comprehensive Phenotypic Investigation of the “Pod-Shattering Syndrome” in Common Bean |
title_short | A Comprehensive Phenotypic Investigation of the “Pod-Shattering Syndrome” in Common Bean |
title_sort | comprehensive phenotypic investigation of the “pod-shattering syndrome” in common bean |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5334323/ https://www.ncbi.nlm.nih.gov/pubmed/28316606 http://dx.doi.org/10.3389/fpls.2017.00251 |
work_keys_str_mv | AT murgiamarial acomprehensivephenotypicinvestigationofthepodshatteringsyndromeincommonbean AT attenegiovanna acomprehensivephenotypicinvestigationofthepodshatteringsyndromeincommonbean AT rodriguezmonica acomprehensivephenotypicinvestigationofthepodshatteringsyndromeincommonbean AT bitocchielena acomprehensivephenotypicinvestigationofthepodshatteringsyndromeincommonbean AT belluccielisa acomprehensivephenotypicinvestigationofthepodshatteringsyndromeincommonbean AT foisdavide acomprehensivephenotypicinvestigationofthepodshatteringsyndromeincommonbean AT nannilaura acomprehensivephenotypicinvestigationofthepodshatteringsyndromeincommonbean AT gioiatania acomprehensivephenotypicinvestigationofthepodshatteringsyndromeincommonbean AT albanidiegom acomprehensivephenotypicinvestigationofthepodshatteringsyndromeincommonbean AT paparoberto acomprehensivephenotypicinvestigationofthepodshatteringsyndromeincommonbean AT raudomenico acomprehensivephenotypicinvestigationofthepodshatteringsyndromeincommonbean AT murgiamarial comprehensivephenotypicinvestigationofthepodshatteringsyndromeincommonbean AT attenegiovanna comprehensivephenotypicinvestigationofthepodshatteringsyndromeincommonbean AT rodriguezmonica comprehensivephenotypicinvestigationofthepodshatteringsyndromeincommonbean AT bitocchielena comprehensivephenotypicinvestigationofthepodshatteringsyndromeincommonbean AT belluccielisa comprehensivephenotypicinvestigationofthepodshatteringsyndromeincommonbean AT foisdavide comprehensivephenotypicinvestigationofthepodshatteringsyndromeincommonbean AT nannilaura comprehensivephenotypicinvestigationofthepodshatteringsyndromeincommonbean AT gioiatania comprehensivephenotypicinvestigationofthepodshatteringsyndromeincommonbean AT albanidiegom comprehensivephenotypicinvestigationofthepodshatteringsyndromeincommonbean AT paparoberto comprehensivephenotypicinvestigationofthepodshatteringsyndromeincommonbean AT raudomenico comprehensivephenotypicinvestigationofthepodshatteringsyndromeincommonbean |