Cargando…
Small-conductance calcium-activated potassium type 2 channels (SK2, KCa2.2) in human brain
SK2 (KCa2.2) channels are voltage-independent Ca(2+)-activated K(+) channels that regulate neuronal excitability in brain regions important for memory formation. In this study, we investigated the distribution and expression of SK2 channels in human brain by Western blot analysis and immunohistochem...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5334391/ https://www.ncbi.nlm.nih.gov/pubmed/27357310 http://dx.doi.org/10.1007/s00429-016-1258-1 |
_version_ | 1782511843736027136 |
---|---|
author | Willis, Michael Trieb, Maria Leitner, Irmgard Wietzorrek, Georg Marksteiner, Josef Knaus, Hans-Günther |
author_facet | Willis, Michael Trieb, Maria Leitner, Irmgard Wietzorrek, Georg Marksteiner, Josef Knaus, Hans-Günther |
author_sort | Willis, Michael |
collection | PubMed |
description | SK2 (KCa2.2) channels are voltage-independent Ca(2+)-activated K(+) channels that regulate neuronal excitability in brain regions important for memory formation. In this study, we investigated the distribution and expression of SK2 channels in human brain by Western blot analysis and immunohistochemistry. Immunoblot analysis of human brain indicated expression of four distinct SK2 channel isoforms: the standard, the long and two short isoforms. Immunohistochemistry in paraffin-embedded post-mortem brain sections was performed in the hippocampal formation, amygdala and neocortex. In hippocampus, SK2-like immunoreactivity could be detected in strata oriens and radiatum of area CA1-CA2 and in the molecular layer. In the amygdala, SK2-like immunoreactivity was highest in the basolateral nuclei, while in neocortex, staining was mainly found enriched in layer V. Activation of SK2 channels is thought to regulate neuronal excitability in brain by contributing to the medium afterhyperpolarization. However, SK2 channels are blocked by apamin with a sensitivity that suggests heteromeric channels. The herein first shown expression of SK2 human isoform b in brain could explain the variability of electrophysiological findings observed with SK2 channels. |
format | Online Article Text |
id | pubmed-5334391 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-53343912017-03-15 Small-conductance calcium-activated potassium type 2 channels (SK2, KCa2.2) in human brain Willis, Michael Trieb, Maria Leitner, Irmgard Wietzorrek, Georg Marksteiner, Josef Knaus, Hans-Günther Brain Struct Funct Original Article SK2 (KCa2.2) channels are voltage-independent Ca(2+)-activated K(+) channels that regulate neuronal excitability in brain regions important for memory formation. In this study, we investigated the distribution and expression of SK2 channels in human brain by Western blot analysis and immunohistochemistry. Immunoblot analysis of human brain indicated expression of four distinct SK2 channel isoforms: the standard, the long and two short isoforms. Immunohistochemistry in paraffin-embedded post-mortem brain sections was performed in the hippocampal formation, amygdala and neocortex. In hippocampus, SK2-like immunoreactivity could be detected in strata oriens and radiatum of area CA1-CA2 and in the molecular layer. In the amygdala, SK2-like immunoreactivity was highest in the basolateral nuclei, while in neocortex, staining was mainly found enriched in layer V. Activation of SK2 channels is thought to regulate neuronal excitability in brain by contributing to the medium afterhyperpolarization. However, SK2 channels are blocked by apamin with a sensitivity that suggests heteromeric channels. The herein first shown expression of SK2 human isoform b in brain could explain the variability of electrophysiological findings observed with SK2 channels. Springer Berlin Heidelberg 2016-06-29 2017 /pmc/articles/PMC5334391/ /pubmed/27357310 http://dx.doi.org/10.1007/s00429-016-1258-1 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Willis, Michael Trieb, Maria Leitner, Irmgard Wietzorrek, Georg Marksteiner, Josef Knaus, Hans-Günther Small-conductance calcium-activated potassium type 2 channels (SK2, KCa2.2) in human brain |
title | Small-conductance calcium-activated potassium type 2 channels (SK2, KCa2.2) in human brain |
title_full | Small-conductance calcium-activated potassium type 2 channels (SK2, KCa2.2) in human brain |
title_fullStr | Small-conductance calcium-activated potassium type 2 channels (SK2, KCa2.2) in human brain |
title_full_unstemmed | Small-conductance calcium-activated potassium type 2 channels (SK2, KCa2.2) in human brain |
title_short | Small-conductance calcium-activated potassium type 2 channels (SK2, KCa2.2) in human brain |
title_sort | small-conductance calcium-activated potassium type 2 channels (sk2, kca2.2) in human brain |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5334391/ https://www.ncbi.nlm.nih.gov/pubmed/27357310 http://dx.doi.org/10.1007/s00429-016-1258-1 |
work_keys_str_mv | AT willismichael smallconductancecalciumactivatedpotassiumtype2channelssk2kca22inhumanbrain AT triebmaria smallconductancecalciumactivatedpotassiumtype2channelssk2kca22inhumanbrain AT leitnerirmgard smallconductancecalciumactivatedpotassiumtype2channelssk2kca22inhumanbrain AT wietzorrekgeorg smallconductancecalciumactivatedpotassiumtype2channelssk2kca22inhumanbrain AT marksteinerjosef smallconductancecalciumactivatedpotassiumtype2channelssk2kca22inhumanbrain AT knaushansgunther smallconductancecalciumactivatedpotassiumtype2channelssk2kca22inhumanbrain |