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flippant–An R package for the automated analysis of fluorescence-based scramblase assays

BACKGROUND: The lipid scrambling activity of protein extracts and purified scramblases is typically measured using a fluorescence-based assay. While the assay has yielded insight into the scramblase activity in crude membrane preparations, functional validation of candidate scramblases, stoichiometr...

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Detalles Bibliográficos
Autores principales: Cotton, Richard J., Ploier, Birgit, Goren, Michael A., Menon, Anant K., Graumann, Johannes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5335725/
https://www.ncbi.nlm.nih.gov/pubmed/28253836
http://dx.doi.org/10.1186/s12859-017-1542-y