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Alternative cDEP Design to Facilitate Cell Isolation for Identification by Raman Spectroscopy

Dielectrophoresis (DEP) uses non-uniform electric fields to cause motion in particles due to the particles’ intrinsic properties. As such, DEP is a well-suited label-free means for cell sorting. Of the various methods of implementing DEP, contactless dielectrophoresis (cDEP) is advantageous as it av...

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Autores principales: Hanson, Cynthia, Vargis, Elizabeth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5335981/
https://www.ncbi.nlm.nih.gov/pubmed/28208767
http://dx.doi.org/10.3390/s17020327
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author Hanson, Cynthia
Vargis, Elizabeth
author_facet Hanson, Cynthia
Vargis, Elizabeth
author_sort Hanson, Cynthia
collection PubMed
description Dielectrophoresis (DEP) uses non-uniform electric fields to cause motion in particles due to the particles’ intrinsic properties. As such, DEP is a well-suited label-free means for cell sorting. Of the various methods of implementing DEP, contactless dielectrophoresis (cDEP) is advantageous as it avoids common problems associated with DEP, such as electrode fouling and electrolysis. Unfortunately, cDEP devices can be difficult to fabricate, replicate, and reuse. In addition, the operating parameters are limited by the dielectric breakdown of polydimethylsiloxane (PDMS). This study presents an alternative way to fabricate a cDEP device allowing for higher operating voltages, improved replication, and the opportunity for analysis using Raman spectroscopy. In this device, channels were formed in fused silica rather than PDMS. The device successfully trapped 3.3 μm polystyrene spheres for analysis by Raman spectroscopy. The successful implementation indicates the potential to use cDEP to isolate and identify biological samples on a single device.
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spelling pubmed-53359812017-03-16 Alternative cDEP Design to Facilitate Cell Isolation for Identification by Raman Spectroscopy Hanson, Cynthia Vargis, Elizabeth Sensors (Basel) Article Dielectrophoresis (DEP) uses non-uniform electric fields to cause motion in particles due to the particles’ intrinsic properties. As such, DEP is a well-suited label-free means for cell sorting. Of the various methods of implementing DEP, contactless dielectrophoresis (cDEP) is advantageous as it avoids common problems associated with DEP, such as electrode fouling and electrolysis. Unfortunately, cDEP devices can be difficult to fabricate, replicate, and reuse. In addition, the operating parameters are limited by the dielectric breakdown of polydimethylsiloxane (PDMS). This study presents an alternative way to fabricate a cDEP device allowing for higher operating voltages, improved replication, and the opportunity for analysis using Raman spectroscopy. In this device, channels were formed in fused silica rather than PDMS. The device successfully trapped 3.3 μm polystyrene spheres for analysis by Raman spectroscopy. The successful implementation indicates the potential to use cDEP to isolate and identify biological samples on a single device. MDPI 2017-02-09 /pmc/articles/PMC5335981/ /pubmed/28208767 http://dx.doi.org/10.3390/s17020327 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hanson, Cynthia
Vargis, Elizabeth
Alternative cDEP Design to Facilitate Cell Isolation for Identification by Raman Spectroscopy
title Alternative cDEP Design to Facilitate Cell Isolation for Identification by Raman Spectroscopy
title_full Alternative cDEP Design to Facilitate Cell Isolation for Identification by Raman Spectroscopy
title_fullStr Alternative cDEP Design to Facilitate Cell Isolation for Identification by Raman Spectroscopy
title_full_unstemmed Alternative cDEP Design to Facilitate Cell Isolation for Identification by Raman Spectroscopy
title_short Alternative cDEP Design to Facilitate Cell Isolation for Identification by Raman Spectroscopy
title_sort alternative cdep design to facilitate cell isolation for identification by raman spectroscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5335981/
https://www.ncbi.nlm.nih.gov/pubmed/28208767
http://dx.doi.org/10.3390/s17020327
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