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Application of the PAMONO-Sensor for Quantification of Microvesicles and Determination of Nano-Particle Size Distribution

The PAMONO-sensor (plasmon assisted microscopy of nano-objects) demonstrated an ability to detect and quantify individual viruses and virus-like particles. However, another group of biological vesicles—microvesicles (100–1000 nm)—also attracts growing interest as biomarkers of different pathologies...

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Autores principales: Shpacovitch, Victoria, Sidorenko, Irina, Lenssen, Jan Eric, Temchura, Vladimir, Weichert, Frank, Müller, Heinrich, Überla, Klaus, Zybin, Alexander, Schramm, Alexander, Hergenröder, Roland
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5336007/
https://www.ncbi.nlm.nih.gov/pubmed/28134825
http://dx.doi.org/10.3390/s17020244
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author Shpacovitch, Victoria
Sidorenko, Irina
Lenssen, Jan Eric
Temchura, Vladimir
Weichert, Frank
Müller, Heinrich
Überla, Klaus
Zybin, Alexander
Schramm, Alexander
Hergenröder, Roland
author_facet Shpacovitch, Victoria
Sidorenko, Irina
Lenssen, Jan Eric
Temchura, Vladimir
Weichert, Frank
Müller, Heinrich
Überla, Klaus
Zybin, Alexander
Schramm, Alexander
Hergenröder, Roland
author_sort Shpacovitch, Victoria
collection PubMed
description The PAMONO-sensor (plasmon assisted microscopy of nano-objects) demonstrated an ability to detect and quantify individual viruses and virus-like particles. However, another group of biological vesicles—microvesicles (100–1000 nm)—also attracts growing interest as biomarkers of different pathologies and needs development of novel techniques for characterization. This work shows the applicability of a PAMONO-sensor for selective detection of microvesicles in aquatic samples. The sensor permits comparison of relative concentrations of microvesicles between samples. We also study a possibility of repeated use of a sensor chip after elution of the microvesicle capturing layer. Moreover, we improve the detection features of the PAMONO-sensor. The detection process utilizes novel machine learning techniques on the sensor image data to estimate particle size distributions of nano-particles in polydisperse samples. Altogether, our findings expand analytical features and the application field of the PAMONO-sensor. They can also serve for a maturation of diagnostic tools based on the PAMONO-sensor platform.
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spelling pubmed-53360072017-03-16 Application of the PAMONO-Sensor for Quantification of Microvesicles and Determination of Nano-Particle Size Distribution Shpacovitch, Victoria Sidorenko, Irina Lenssen, Jan Eric Temchura, Vladimir Weichert, Frank Müller, Heinrich Überla, Klaus Zybin, Alexander Schramm, Alexander Hergenröder, Roland Sensors (Basel) Article The PAMONO-sensor (plasmon assisted microscopy of nano-objects) demonstrated an ability to detect and quantify individual viruses and virus-like particles. However, another group of biological vesicles—microvesicles (100–1000 nm)—also attracts growing interest as biomarkers of different pathologies and needs development of novel techniques for characterization. This work shows the applicability of a PAMONO-sensor for selective detection of microvesicles in aquatic samples. The sensor permits comparison of relative concentrations of microvesicles between samples. We also study a possibility of repeated use of a sensor chip after elution of the microvesicle capturing layer. Moreover, we improve the detection features of the PAMONO-sensor. The detection process utilizes novel machine learning techniques on the sensor image data to estimate particle size distributions of nano-particles in polydisperse samples. Altogether, our findings expand analytical features and the application field of the PAMONO-sensor. They can also serve for a maturation of diagnostic tools based on the PAMONO-sensor platform. MDPI 2017-01-27 /pmc/articles/PMC5336007/ /pubmed/28134825 http://dx.doi.org/10.3390/s17020244 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shpacovitch, Victoria
Sidorenko, Irina
Lenssen, Jan Eric
Temchura, Vladimir
Weichert, Frank
Müller, Heinrich
Überla, Klaus
Zybin, Alexander
Schramm, Alexander
Hergenröder, Roland
Application of the PAMONO-Sensor for Quantification of Microvesicles and Determination of Nano-Particle Size Distribution
title Application of the PAMONO-Sensor for Quantification of Microvesicles and Determination of Nano-Particle Size Distribution
title_full Application of the PAMONO-Sensor for Quantification of Microvesicles and Determination of Nano-Particle Size Distribution
title_fullStr Application of the PAMONO-Sensor for Quantification of Microvesicles and Determination of Nano-Particle Size Distribution
title_full_unstemmed Application of the PAMONO-Sensor for Quantification of Microvesicles and Determination of Nano-Particle Size Distribution
title_short Application of the PAMONO-Sensor for Quantification of Microvesicles and Determination of Nano-Particle Size Distribution
title_sort application of the pamono-sensor for quantification of microvesicles and determination of nano-particle size distribution
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5336007/
https://www.ncbi.nlm.nih.gov/pubmed/28134825
http://dx.doi.org/10.3390/s17020244
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