An Electrochemical Gas Biosensor Based on Enzymes Immobilized on Chromatography Paper for Ethanol Vapor Detection

This paper presents a novel method of fabricating an enzymatic biosensor for breath analysis using chromatography paper as enzyme supporting layer and a liquid phase layer on top of screen printed carbon electrodes. We evaluated the performance with ethanol vapor being one of the breathing ingredien...

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Autores principales: Kuretake, Tatsumi, Kawahara, Shogo, Motooka, Masanobu, Uno, Shigeyasu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5336011/
https://www.ncbi.nlm.nih.gov/pubmed/28157154
http://dx.doi.org/10.3390/s17020281
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author Kuretake, Tatsumi
Kawahara, Shogo
Motooka, Masanobu
Uno, Shigeyasu
author_facet Kuretake, Tatsumi
Kawahara, Shogo
Motooka, Masanobu
Uno, Shigeyasu
author_sort Kuretake, Tatsumi
collection PubMed
description This paper presents a novel method of fabricating an enzymatic biosensor for breath analysis using chromatography paper as enzyme supporting layer and a liquid phase layer on top of screen printed carbon electrodes. We evaluated the performance with ethanol vapor being one of the breathing ingredients. The experimental results show that our sensor is able to measure the concentration of ethanol vapor within the range of 50 to 500 ppm. These results suggest the ability of detecting breath ethanol, and it can possibly be applied as a generic vapor biosensor to a wide range of diseases.
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spelling pubmed-53360112017-03-16 An Electrochemical Gas Biosensor Based on Enzymes Immobilized on Chromatography Paper for Ethanol Vapor Detection Kuretake, Tatsumi Kawahara, Shogo Motooka, Masanobu Uno, Shigeyasu Sensors (Basel) Article This paper presents a novel method of fabricating an enzymatic biosensor for breath analysis using chromatography paper as enzyme supporting layer and a liquid phase layer on top of screen printed carbon electrodes. We evaluated the performance with ethanol vapor being one of the breathing ingredients. The experimental results show that our sensor is able to measure the concentration of ethanol vapor within the range of 50 to 500 ppm. These results suggest the ability of detecting breath ethanol, and it can possibly be applied as a generic vapor biosensor to a wide range of diseases. MDPI 2017-02-01 /pmc/articles/PMC5336011/ /pubmed/28157154 http://dx.doi.org/10.3390/s17020281 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kuretake, Tatsumi
Kawahara, Shogo
Motooka, Masanobu
Uno, Shigeyasu
An Electrochemical Gas Biosensor Based on Enzymes Immobilized on Chromatography Paper for Ethanol Vapor Detection
title An Electrochemical Gas Biosensor Based on Enzymes Immobilized on Chromatography Paper for Ethanol Vapor Detection
title_full An Electrochemical Gas Biosensor Based on Enzymes Immobilized on Chromatography Paper for Ethanol Vapor Detection
title_fullStr An Electrochemical Gas Biosensor Based on Enzymes Immobilized on Chromatography Paper for Ethanol Vapor Detection
title_full_unstemmed An Electrochemical Gas Biosensor Based on Enzymes Immobilized on Chromatography Paper for Ethanol Vapor Detection
title_short An Electrochemical Gas Biosensor Based on Enzymes Immobilized on Chromatography Paper for Ethanol Vapor Detection
title_sort electrochemical gas biosensor based on enzymes immobilized on chromatography paper for ethanol vapor detection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5336011/
https://www.ncbi.nlm.nih.gov/pubmed/28157154
http://dx.doi.org/10.3390/s17020281
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