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Dendritic stratification differs among retinal OFF bipolar cell types in the absence of rod photoreceptors

Retinal OFF bipolar cells show distinct connectivity patterns with photoreceptors in the wild-type mouse retina. Some types are cone-specific while others penetrate further through the outer plexiform layer (OPL) to contact rods in addition to cones. To explore dendritic stratification of OFF bipola...

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Autores principales: Puller, Christian, Arbogast, Patrick, Keeley, Patrick W., Reese, Benjamin E., Haverkamp, Silke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5336283/
https://www.ncbi.nlm.nih.gov/pubmed/28257490
http://dx.doi.org/10.1371/journal.pone.0173455
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author Puller, Christian
Arbogast, Patrick
Keeley, Patrick W.
Reese, Benjamin E.
Haverkamp, Silke
author_facet Puller, Christian
Arbogast, Patrick
Keeley, Patrick W.
Reese, Benjamin E.
Haverkamp, Silke
author_sort Puller, Christian
collection PubMed
description Retinal OFF bipolar cells show distinct connectivity patterns with photoreceptors in the wild-type mouse retina. Some types are cone-specific while others penetrate further through the outer plexiform layer (OPL) to contact rods in addition to cones. To explore dendritic stratification of OFF bipolar cells in the absence of rods, we made use of the ‘cone-full’ Nrl(-/-) mouse retina in which all photoreceptor precursor cells commit to a cone fate including those which would have become rods in wild-type retinas. The dendritic distribution of OFF bipolar cell types was investigated by confocal and electron microscopic imaging of immunolabeled tissue sections. The cells’ dendrites formed basal contacts with cone terminals and expressed the corresponding glutamate receptor subunits at those sites, indicating putative synapses. All of the four analyzed cell populations showed distinctive patterns of vertical dendritic invasion through the OPL. This disparate behavior of dendritic extension in an environment containing only cone terminals demonstrates type-dependent specificity for dendritic outgrowth in OFF bipolar cells: rod terminals are not required for inducing dendritic extension into distal areas of the OPL.
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spelling pubmed-53362832017-03-10 Dendritic stratification differs among retinal OFF bipolar cell types in the absence of rod photoreceptors Puller, Christian Arbogast, Patrick Keeley, Patrick W. Reese, Benjamin E. Haverkamp, Silke PLoS One Research Article Retinal OFF bipolar cells show distinct connectivity patterns with photoreceptors in the wild-type mouse retina. Some types are cone-specific while others penetrate further through the outer plexiform layer (OPL) to contact rods in addition to cones. To explore dendritic stratification of OFF bipolar cells in the absence of rods, we made use of the ‘cone-full’ Nrl(-/-) mouse retina in which all photoreceptor precursor cells commit to a cone fate including those which would have become rods in wild-type retinas. The dendritic distribution of OFF bipolar cell types was investigated by confocal and electron microscopic imaging of immunolabeled tissue sections. The cells’ dendrites formed basal contacts with cone terminals and expressed the corresponding glutamate receptor subunits at those sites, indicating putative synapses. All of the four analyzed cell populations showed distinctive patterns of vertical dendritic invasion through the OPL. This disparate behavior of dendritic extension in an environment containing only cone terminals demonstrates type-dependent specificity for dendritic outgrowth in OFF bipolar cells: rod terminals are not required for inducing dendritic extension into distal areas of the OPL. Public Library of Science 2017-03-03 /pmc/articles/PMC5336283/ /pubmed/28257490 http://dx.doi.org/10.1371/journal.pone.0173455 Text en © 2017 Puller et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Puller, Christian
Arbogast, Patrick
Keeley, Patrick W.
Reese, Benjamin E.
Haverkamp, Silke
Dendritic stratification differs among retinal OFF bipolar cell types in the absence of rod photoreceptors
title Dendritic stratification differs among retinal OFF bipolar cell types in the absence of rod photoreceptors
title_full Dendritic stratification differs among retinal OFF bipolar cell types in the absence of rod photoreceptors
title_fullStr Dendritic stratification differs among retinal OFF bipolar cell types in the absence of rod photoreceptors
title_full_unstemmed Dendritic stratification differs among retinal OFF bipolar cell types in the absence of rod photoreceptors
title_short Dendritic stratification differs among retinal OFF bipolar cell types in the absence of rod photoreceptors
title_sort dendritic stratification differs among retinal off bipolar cell types in the absence of rod photoreceptors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5336283/
https://www.ncbi.nlm.nih.gov/pubmed/28257490
http://dx.doi.org/10.1371/journal.pone.0173455
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