Cargando…

New in vitro system to predict chemotherapeutic efficacy of drug combinations in fresh tumor samples

BACKGROUND: To find the best individual chemotherapy for cancer patients, the efficacy of different chemotherapeutic drugs can be predicted by pretesting tumor samples in vitro via the chemotherapy-resistance (CTR)-Test(®). Although drug combinations are widely used among cancer therapy, so far only...

Descripción completa

Detalles Bibliográficos
Autores principales: Kischkel, Frank Christian, Eich, Julia, Meyer, Carina I., Weidemüller, Paula, Krapfl, Jens, Yassin-Kelepir, Rauaa, Job, Laura, Fraefel, Marius, Braicu, Ioana, Kopp-Schneider, Annette, Sehouli, Jalid, De Wilde, Rudy Leon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5337084/
https://www.ncbi.nlm.nih.gov/pubmed/28265509
http://dx.doi.org/10.7717/peerj.3030
_version_ 1782512330251173888
author Kischkel, Frank Christian
Eich, Julia
Meyer, Carina I.
Weidemüller, Paula
Krapfl, Jens
Yassin-Kelepir, Rauaa
Job, Laura
Fraefel, Marius
Braicu, Ioana
Kopp-Schneider, Annette
Sehouli, Jalid
De Wilde, Rudy Leon
author_facet Kischkel, Frank Christian
Eich, Julia
Meyer, Carina I.
Weidemüller, Paula
Krapfl, Jens
Yassin-Kelepir, Rauaa
Job, Laura
Fraefel, Marius
Braicu, Ioana
Kopp-Schneider, Annette
Sehouli, Jalid
De Wilde, Rudy Leon
author_sort Kischkel, Frank Christian
collection PubMed
description BACKGROUND: To find the best individual chemotherapy for cancer patients, the efficacy of different chemotherapeutic drugs can be predicted by pretesting tumor samples in vitro via the chemotherapy-resistance (CTR)-Test(®). Although drug combinations are widely used among cancer therapy, so far only single drugs are tested by this and other tests. However, several first line chemotherapies are combining two or more chemotherapeutics, leading to the necessity of drug combination testing methods. METHODS: We established a system to measure and predict the efficacy of chemotherapeutic drug combinations with the help of the Loewe additivity concept in combination with the CTR-test. A combination is measured by using half of the monotherapy’s concentration of both drugs simultaneously. With this method, the efficacy of a combination can also be calculated based on single drug measurements. RESULTS: The established system was tested on a data set of ovarian carcinoma samples using the combination carboplatin and paclitaxel and confirmed by using other tumor species and chemotherapeutics. Comparing the measured and the calculated values of the combination testings revealed a high correlation. Additionally, in 70% of the cases the measured and the calculated values lead to the same chemotherapeutic resistance category of the tumor. CONCLUSION: Our data suggest that the best drug combination consists of the most efficient single drugs and the worst drug combination of the least efficient single drugs. Our results showed that single measurements are sufficient to predict combinations in specific cases but there are exceptions in which it is necessary to measure combinations, which is possible with the presented system.
format Online
Article
Text
id pubmed-5337084
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher PeerJ Inc.
record_format MEDLINE/PubMed
spelling pubmed-53370842017-03-06 New in vitro system to predict chemotherapeutic efficacy of drug combinations in fresh tumor samples Kischkel, Frank Christian Eich, Julia Meyer, Carina I. Weidemüller, Paula Krapfl, Jens Yassin-Kelepir, Rauaa Job, Laura Fraefel, Marius Braicu, Ioana Kopp-Schneider, Annette Sehouli, Jalid De Wilde, Rudy Leon PeerJ Gynecology and Obstetrics BACKGROUND: To find the best individual chemotherapy for cancer patients, the efficacy of different chemotherapeutic drugs can be predicted by pretesting tumor samples in vitro via the chemotherapy-resistance (CTR)-Test(®). Although drug combinations are widely used among cancer therapy, so far only single drugs are tested by this and other tests. However, several first line chemotherapies are combining two or more chemotherapeutics, leading to the necessity of drug combination testing methods. METHODS: We established a system to measure and predict the efficacy of chemotherapeutic drug combinations with the help of the Loewe additivity concept in combination with the CTR-test. A combination is measured by using half of the monotherapy’s concentration of both drugs simultaneously. With this method, the efficacy of a combination can also be calculated based on single drug measurements. RESULTS: The established system was tested on a data set of ovarian carcinoma samples using the combination carboplatin and paclitaxel and confirmed by using other tumor species and chemotherapeutics. Comparing the measured and the calculated values of the combination testings revealed a high correlation. Additionally, in 70% of the cases the measured and the calculated values lead to the same chemotherapeutic resistance category of the tumor. CONCLUSION: Our data suggest that the best drug combination consists of the most efficient single drugs and the worst drug combination of the least efficient single drugs. Our results showed that single measurements are sufficient to predict combinations in specific cases but there are exceptions in which it is necessary to measure combinations, which is possible with the presented system. PeerJ Inc. 2017-03-02 /pmc/articles/PMC5337084/ /pubmed/28265509 http://dx.doi.org/10.7717/peerj.3030 Text en ©2017 Kischkel et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Gynecology and Obstetrics
Kischkel, Frank Christian
Eich, Julia
Meyer, Carina I.
Weidemüller, Paula
Krapfl, Jens
Yassin-Kelepir, Rauaa
Job, Laura
Fraefel, Marius
Braicu, Ioana
Kopp-Schneider, Annette
Sehouli, Jalid
De Wilde, Rudy Leon
New in vitro system to predict chemotherapeutic efficacy of drug combinations in fresh tumor samples
title New in vitro system to predict chemotherapeutic efficacy of drug combinations in fresh tumor samples
title_full New in vitro system to predict chemotherapeutic efficacy of drug combinations in fresh tumor samples
title_fullStr New in vitro system to predict chemotherapeutic efficacy of drug combinations in fresh tumor samples
title_full_unstemmed New in vitro system to predict chemotherapeutic efficacy of drug combinations in fresh tumor samples
title_short New in vitro system to predict chemotherapeutic efficacy of drug combinations in fresh tumor samples
title_sort new in vitro system to predict chemotherapeutic efficacy of drug combinations in fresh tumor samples
topic Gynecology and Obstetrics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5337084/
https://www.ncbi.nlm.nih.gov/pubmed/28265509
http://dx.doi.org/10.7717/peerj.3030
work_keys_str_mv AT kischkelfrankchristian newinvitrosystemtopredictchemotherapeuticefficacyofdrugcombinationsinfreshtumorsamples
AT eichjulia newinvitrosystemtopredictchemotherapeuticefficacyofdrugcombinationsinfreshtumorsamples
AT meyercarinai newinvitrosystemtopredictchemotherapeuticefficacyofdrugcombinationsinfreshtumorsamples
AT weidemullerpaula newinvitrosystemtopredictchemotherapeuticefficacyofdrugcombinationsinfreshtumorsamples
AT krapfljens newinvitrosystemtopredictchemotherapeuticefficacyofdrugcombinationsinfreshtumorsamples
AT yassinkelepirrauaa newinvitrosystemtopredictchemotherapeuticefficacyofdrugcombinationsinfreshtumorsamples
AT joblaura newinvitrosystemtopredictchemotherapeuticefficacyofdrugcombinationsinfreshtumorsamples
AT fraefelmarius newinvitrosystemtopredictchemotherapeuticefficacyofdrugcombinationsinfreshtumorsamples
AT braicuioana newinvitrosystemtopredictchemotherapeuticefficacyofdrugcombinationsinfreshtumorsamples
AT koppschneiderannette newinvitrosystemtopredictchemotherapeuticefficacyofdrugcombinationsinfreshtumorsamples
AT sehoulijalid newinvitrosystemtopredictchemotherapeuticefficacyofdrugcombinationsinfreshtumorsamples
AT dewilderudyleon newinvitrosystemtopredictchemotherapeuticefficacyofdrugcombinationsinfreshtumorsamples