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New in vitro system to predict chemotherapeutic efficacy of drug combinations in fresh tumor samples
BACKGROUND: To find the best individual chemotherapy for cancer patients, the efficacy of different chemotherapeutic drugs can be predicted by pretesting tumor samples in vitro via the chemotherapy-resistance (CTR)-Test(®). Although drug combinations are widely used among cancer therapy, so far only...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5337084/ https://www.ncbi.nlm.nih.gov/pubmed/28265509 http://dx.doi.org/10.7717/peerj.3030 |
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author | Kischkel, Frank Christian Eich, Julia Meyer, Carina I. Weidemüller, Paula Krapfl, Jens Yassin-Kelepir, Rauaa Job, Laura Fraefel, Marius Braicu, Ioana Kopp-Schneider, Annette Sehouli, Jalid De Wilde, Rudy Leon |
author_facet | Kischkel, Frank Christian Eich, Julia Meyer, Carina I. Weidemüller, Paula Krapfl, Jens Yassin-Kelepir, Rauaa Job, Laura Fraefel, Marius Braicu, Ioana Kopp-Schneider, Annette Sehouli, Jalid De Wilde, Rudy Leon |
author_sort | Kischkel, Frank Christian |
collection | PubMed |
description | BACKGROUND: To find the best individual chemotherapy for cancer patients, the efficacy of different chemotherapeutic drugs can be predicted by pretesting tumor samples in vitro via the chemotherapy-resistance (CTR)-Test(®). Although drug combinations are widely used among cancer therapy, so far only single drugs are tested by this and other tests. However, several first line chemotherapies are combining two or more chemotherapeutics, leading to the necessity of drug combination testing methods. METHODS: We established a system to measure and predict the efficacy of chemotherapeutic drug combinations with the help of the Loewe additivity concept in combination with the CTR-test. A combination is measured by using half of the monotherapy’s concentration of both drugs simultaneously. With this method, the efficacy of a combination can also be calculated based on single drug measurements. RESULTS: The established system was tested on a data set of ovarian carcinoma samples using the combination carboplatin and paclitaxel and confirmed by using other tumor species and chemotherapeutics. Comparing the measured and the calculated values of the combination testings revealed a high correlation. Additionally, in 70% of the cases the measured and the calculated values lead to the same chemotherapeutic resistance category of the tumor. CONCLUSION: Our data suggest that the best drug combination consists of the most efficient single drugs and the worst drug combination of the least efficient single drugs. Our results showed that single measurements are sufficient to predict combinations in specific cases but there are exceptions in which it is necessary to measure combinations, which is possible with the presented system. |
format | Online Article Text |
id | pubmed-5337084 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-53370842017-03-06 New in vitro system to predict chemotherapeutic efficacy of drug combinations in fresh tumor samples Kischkel, Frank Christian Eich, Julia Meyer, Carina I. Weidemüller, Paula Krapfl, Jens Yassin-Kelepir, Rauaa Job, Laura Fraefel, Marius Braicu, Ioana Kopp-Schneider, Annette Sehouli, Jalid De Wilde, Rudy Leon PeerJ Gynecology and Obstetrics BACKGROUND: To find the best individual chemotherapy for cancer patients, the efficacy of different chemotherapeutic drugs can be predicted by pretesting tumor samples in vitro via the chemotherapy-resistance (CTR)-Test(®). Although drug combinations are widely used among cancer therapy, so far only single drugs are tested by this and other tests. However, several first line chemotherapies are combining two or more chemotherapeutics, leading to the necessity of drug combination testing methods. METHODS: We established a system to measure and predict the efficacy of chemotherapeutic drug combinations with the help of the Loewe additivity concept in combination with the CTR-test. A combination is measured by using half of the monotherapy’s concentration of both drugs simultaneously. With this method, the efficacy of a combination can also be calculated based on single drug measurements. RESULTS: The established system was tested on a data set of ovarian carcinoma samples using the combination carboplatin and paclitaxel and confirmed by using other tumor species and chemotherapeutics. Comparing the measured and the calculated values of the combination testings revealed a high correlation. Additionally, in 70% of the cases the measured and the calculated values lead to the same chemotherapeutic resistance category of the tumor. CONCLUSION: Our data suggest that the best drug combination consists of the most efficient single drugs and the worst drug combination of the least efficient single drugs. Our results showed that single measurements are sufficient to predict combinations in specific cases but there are exceptions in which it is necessary to measure combinations, which is possible with the presented system. PeerJ Inc. 2017-03-02 /pmc/articles/PMC5337084/ /pubmed/28265509 http://dx.doi.org/10.7717/peerj.3030 Text en ©2017 Kischkel et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Gynecology and Obstetrics Kischkel, Frank Christian Eich, Julia Meyer, Carina I. Weidemüller, Paula Krapfl, Jens Yassin-Kelepir, Rauaa Job, Laura Fraefel, Marius Braicu, Ioana Kopp-Schneider, Annette Sehouli, Jalid De Wilde, Rudy Leon New in vitro system to predict chemotherapeutic efficacy of drug combinations in fresh tumor samples |
title | New in vitro system to predict chemotherapeutic efficacy of drug combinations in fresh tumor samples |
title_full | New in vitro system to predict chemotherapeutic efficacy of drug combinations in fresh tumor samples |
title_fullStr | New in vitro system to predict chemotherapeutic efficacy of drug combinations in fresh tumor samples |
title_full_unstemmed | New in vitro system to predict chemotherapeutic efficacy of drug combinations in fresh tumor samples |
title_short | New in vitro system to predict chemotherapeutic efficacy of drug combinations in fresh tumor samples |
title_sort | new in vitro system to predict chemotherapeutic efficacy of drug combinations in fresh tumor samples |
topic | Gynecology and Obstetrics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5337084/ https://www.ncbi.nlm.nih.gov/pubmed/28265509 http://dx.doi.org/10.7717/peerj.3030 |
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