Cargando…
Multiple Kinases Involved in the Nicotinic Modulation of Gamma Oscillations in the Rat Hippocampal CA3 Area
Neuronal synchronization at gamma band frequency (20–80 Hz, γ oscillations) is closely associated with higher brain function, such as learning, memory and attention. Nicotinic acetylcholine receptors (nAChRs) are highly expressed in the hippocampus, and modulate hippocampal γ oscillations, but the i...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5337687/ https://www.ncbi.nlm.nih.gov/pubmed/28321180 http://dx.doi.org/10.3389/fncel.2017.00057 |
_version_ | 1782512417741209600 |
---|---|
author | Wang, JianGang He, XiaoLong Guo, Fangli Cheng, XiangLin Wang, Yali Wang, XiaoFang Feng, ZhiWei Vreugdenhil, Martin Lu, ChengBiao |
author_facet | Wang, JianGang He, XiaoLong Guo, Fangli Cheng, XiangLin Wang, Yali Wang, XiaoFang Feng, ZhiWei Vreugdenhil, Martin Lu, ChengBiao |
author_sort | Wang, JianGang |
collection | PubMed |
description | Neuronal synchronization at gamma band frequency (20–80 Hz, γ oscillations) is closely associated with higher brain function, such as learning, memory and attention. Nicotinic acetylcholine receptors (nAChRs) are highly expressed in the hippocampus, and modulate hippocampal γ oscillations, but the intracellular mechanism underlying such modulation remains elusive. We explored multiple kinases by which nicotine can modulate γ oscillations induced by kainate in rat hippocampal area CA3 in vitro. We found that inhibitors of cyclic AMP dependent kinase (protein kinase A, PKA), protein kinase C (PKC), N-methyl-D-aspartate receptor (NMDA) receptors, Phosphoinositide 3-kinase (PI3K) and extracellular signal-related kinases (ERK), each individually could prevent the γ oscillation-enhancing effect of 1 μM nicotine, whereas none of them affected baseline γ oscillation strength. Inhibition of the serine/threonine kinase Akt increased baseline γ oscillations and partially blocked its nicotinic enhancement. We propose that the PKA-NMDAR-PI3K-ERK pathway modifies cellular properties required for the nicotinic enhancement of γ oscillations, dependent on a PKC-ERK mediated pathway. These signaling pathways provide clues for restoring γ oscillations in pathological conditions affecting cognition. The suppression of γ oscillations at 100 μM nicotine was only dependent on PKA-NMDAR activation and may be due to very high intracellular calcium levels. |
format | Online Article Text |
id | pubmed-5337687 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-53376872017-03-20 Multiple Kinases Involved in the Nicotinic Modulation of Gamma Oscillations in the Rat Hippocampal CA3 Area Wang, JianGang He, XiaoLong Guo, Fangli Cheng, XiangLin Wang, Yali Wang, XiaoFang Feng, ZhiWei Vreugdenhil, Martin Lu, ChengBiao Front Cell Neurosci Neuroscience Neuronal synchronization at gamma band frequency (20–80 Hz, γ oscillations) is closely associated with higher brain function, such as learning, memory and attention. Nicotinic acetylcholine receptors (nAChRs) are highly expressed in the hippocampus, and modulate hippocampal γ oscillations, but the intracellular mechanism underlying such modulation remains elusive. We explored multiple kinases by which nicotine can modulate γ oscillations induced by kainate in rat hippocampal area CA3 in vitro. We found that inhibitors of cyclic AMP dependent kinase (protein kinase A, PKA), protein kinase C (PKC), N-methyl-D-aspartate receptor (NMDA) receptors, Phosphoinositide 3-kinase (PI3K) and extracellular signal-related kinases (ERK), each individually could prevent the γ oscillation-enhancing effect of 1 μM nicotine, whereas none of them affected baseline γ oscillation strength. Inhibition of the serine/threonine kinase Akt increased baseline γ oscillations and partially blocked its nicotinic enhancement. We propose that the PKA-NMDAR-PI3K-ERK pathway modifies cellular properties required for the nicotinic enhancement of γ oscillations, dependent on a PKC-ERK mediated pathway. These signaling pathways provide clues for restoring γ oscillations in pathological conditions affecting cognition. The suppression of γ oscillations at 100 μM nicotine was only dependent on PKA-NMDAR activation and may be due to very high intracellular calcium levels. Frontiers Media S.A. 2017-03-06 /pmc/articles/PMC5337687/ /pubmed/28321180 http://dx.doi.org/10.3389/fncel.2017.00057 Text en Copyright © 2017 Wang, He, Guo, Cheng, Wang, Wang, Feng, Vreugdenhil and Lu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Wang, JianGang He, XiaoLong Guo, Fangli Cheng, XiangLin Wang, Yali Wang, XiaoFang Feng, ZhiWei Vreugdenhil, Martin Lu, ChengBiao Multiple Kinases Involved in the Nicotinic Modulation of Gamma Oscillations in the Rat Hippocampal CA3 Area |
title | Multiple Kinases Involved in the Nicotinic Modulation of Gamma Oscillations in the Rat Hippocampal CA3 Area |
title_full | Multiple Kinases Involved in the Nicotinic Modulation of Gamma Oscillations in the Rat Hippocampal CA3 Area |
title_fullStr | Multiple Kinases Involved in the Nicotinic Modulation of Gamma Oscillations in the Rat Hippocampal CA3 Area |
title_full_unstemmed | Multiple Kinases Involved in the Nicotinic Modulation of Gamma Oscillations in the Rat Hippocampal CA3 Area |
title_short | Multiple Kinases Involved in the Nicotinic Modulation of Gamma Oscillations in the Rat Hippocampal CA3 Area |
title_sort | multiple kinases involved in the nicotinic modulation of gamma oscillations in the rat hippocampal ca3 area |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5337687/ https://www.ncbi.nlm.nih.gov/pubmed/28321180 http://dx.doi.org/10.3389/fncel.2017.00057 |
work_keys_str_mv | AT wangjiangang multiplekinasesinvolvedinthenicotinicmodulationofgammaoscillationsintherathippocampalca3area AT hexiaolong multiplekinasesinvolvedinthenicotinicmodulationofgammaoscillationsintherathippocampalca3area AT guofangli multiplekinasesinvolvedinthenicotinicmodulationofgammaoscillationsintherathippocampalca3area AT chengxianglin multiplekinasesinvolvedinthenicotinicmodulationofgammaoscillationsintherathippocampalca3area AT wangyali multiplekinasesinvolvedinthenicotinicmodulationofgammaoscillationsintherathippocampalca3area AT wangxiaofang multiplekinasesinvolvedinthenicotinicmodulationofgammaoscillationsintherathippocampalca3area AT fengzhiwei multiplekinasesinvolvedinthenicotinicmodulationofgammaoscillationsintherathippocampalca3area AT vreugdenhilmartin multiplekinasesinvolvedinthenicotinicmodulationofgammaoscillationsintherathippocampalca3area AT luchengbiao multiplekinasesinvolvedinthenicotinicmodulationofgammaoscillationsintherathippocampalca3area |