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Cytotoxicity and Antimicrobial Effects of a New Fast-Set MTA

Purpose. To compare the biocompatibility and antimicrobial effectiveness of the new Fast-Set MTA (FS-MTA) with ProRoot MTA (RS-MTA). Methods. The agar overlay method with neutral red dye was used. L929 mouse fibroblast cells were cultured. The liquid and oil extracts and solid test material were pla...

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Autores principales: Shin, Michelle, Chen, Jung-Wei, Tsai, Chi-Yang, Aprecio, Raydolfo, Zhang, Wu, Yochim, Ji Min, Teng, Naichia, Torabinejad, Mahmoud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5337838/
https://www.ncbi.nlm.nih.gov/pubmed/28303246
http://dx.doi.org/10.1155/2017/2071247
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author Shin, Michelle
Chen, Jung-Wei
Tsai, Chi-Yang
Aprecio, Raydolfo
Zhang, Wu
Yochim, Ji Min
Teng, Naichia
Torabinejad, Mahmoud
author_facet Shin, Michelle
Chen, Jung-Wei
Tsai, Chi-Yang
Aprecio, Raydolfo
Zhang, Wu
Yochim, Ji Min
Teng, Naichia
Torabinejad, Mahmoud
author_sort Shin, Michelle
collection PubMed
description Purpose. To compare the biocompatibility and antimicrobial effectiveness of the new Fast-Set MTA (FS-MTA) with ProRoot MTA (RS-MTA). Methods. The agar overlay method with neutral red dye was used. L929 mouse fibroblast cells were cultured. The liquid and oil extracts and solid test material were placed on the agar overlay, four samples for each material. Phenol was used as the positive control and cottonseed oil and MEM extracts were used as negative controls. Cytotoxicity was examined by measuring the zones of decolorization and evaluating cell lysis under an inverted microscope using the established criteria after 24 and 48 hours. The antimicrobial test was performed using the Kirby-Bauer disk-diffusion method against S. mutans, E. faecalis, F. nucleatum, P. gingivalis, and P. intermedia. The size of the zone of inhibition was measured in millimeters. Results. There was no zone of decolorization seen under or around the test materials for FS-MTA and RS-MTA at 24 and 48 hours. The antimicrobial test demonstrated no inhibitory effect of FS-MTA or RS-MTA on any bacterial species after 24 and 48 hours. Conclusions. There was no cytotoxicity or bacterial inhibition observed by the new Fast-Set MTA when compared to the ProRoot MTA after setting.
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spelling pubmed-53378382017-03-16 Cytotoxicity and Antimicrobial Effects of a New Fast-Set MTA Shin, Michelle Chen, Jung-Wei Tsai, Chi-Yang Aprecio, Raydolfo Zhang, Wu Yochim, Ji Min Teng, Naichia Torabinejad, Mahmoud Biomed Res Int Research Article Purpose. To compare the biocompatibility and antimicrobial effectiveness of the new Fast-Set MTA (FS-MTA) with ProRoot MTA (RS-MTA). Methods. The agar overlay method with neutral red dye was used. L929 mouse fibroblast cells were cultured. The liquid and oil extracts and solid test material were placed on the agar overlay, four samples for each material. Phenol was used as the positive control and cottonseed oil and MEM extracts were used as negative controls. Cytotoxicity was examined by measuring the zones of decolorization and evaluating cell lysis under an inverted microscope using the established criteria after 24 and 48 hours. The antimicrobial test was performed using the Kirby-Bauer disk-diffusion method against S. mutans, E. faecalis, F. nucleatum, P. gingivalis, and P. intermedia. The size of the zone of inhibition was measured in millimeters. Results. There was no zone of decolorization seen under or around the test materials for FS-MTA and RS-MTA at 24 and 48 hours. The antimicrobial test demonstrated no inhibitory effect of FS-MTA or RS-MTA on any bacterial species after 24 and 48 hours. Conclusions. There was no cytotoxicity or bacterial inhibition observed by the new Fast-Set MTA when compared to the ProRoot MTA after setting. Hindawi Publishing Corporation 2017 2017-02-20 /pmc/articles/PMC5337838/ /pubmed/28303246 http://dx.doi.org/10.1155/2017/2071247 Text en Copyright © 2017 Michelle Shin et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Shin, Michelle
Chen, Jung-Wei
Tsai, Chi-Yang
Aprecio, Raydolfo
Zhang, Wu
Yochim, Ji Min
Teng, Naichia
Torabinejad, Mahmoud
Cytotoxicity and Antimicrobial Effects of a New Fast-Set MTA
title Cytotoxicity and Antimicrobial Effects of a New Fast-Set MTA
title_full Cytotoxicity and Antimicrobial Effects of a New Fast-Set MTA
title_fullStr Cytotoxicity and Antimicrobial Effects of a New Fast-Set MTA
title_full_unstemmed Cytotoxicity and Antimicrobial Effects of a New Fast-Set MTA
title_short Cytotoxicity and Antimicrobial Effects of a New Fast-Set MTA
title_sort cytotoxicity and antimicrobial effects of a new fast-set mta
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5337838/
https://www.ncbi.nlm.nih.gov/pubmed/28303246
http://dx.doi.org/10.1155/2017/2071247
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