Cargando…

Effect of Wnt Signaling on the Differentiation of Islet β-Cells from Adipose-Derived Stem Cells

The Wnt signaling is critical for pancreatic development and islet function; however, its precise effects on the development and function of the β-cells remain controversial. Here we examined mRNA and protein expression of components of the Wnt signaling throughout the differentiation of islet β-cel...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Hefei, Ren, Yu, Hu, Xiao, Ma, Min, Wang, Xiao, Liang, Hao, Liu, Dongjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5337876/
https://www.ncbi.nlm.nih.gov/pubmed/28303247
http://dx.doi.org/10.1155/2017/2501578
_version_ 1782512456984166400
author Wang, Hefei
Ren, Yu
Hu, Xiao
Ma, Min
Wang, Xiao
Liang, Hao
Liu, Dongjun
author_facet Wang, Hefei
Ren, Yu
Hu, Xiao
Ma, Min
Wang, Xiao
Liang, Hao
Liu, Dongjun
author_sort Wang, Hefei
collection PubMed
description The Wnt signaling is critical for pancreatic development and islet function; however, its precise effects on the development and function of the β-cells remain controversial. Here we examined mRNA and protein expression of components of the Wnt signaling throughout the differentiation of islet β-cells from adipose-derived stem cells (ADSCs). After induction, ADSCs expressed markers of β-cells, including the insulin, PDX1, and glucagon genes, and the PDX1, CK19, nestin, insulin, and C-peptide proteins, indicating their successful differentiation. Compared with pancreatic adult stem cells (PASCs), the quantities of insulin, GLUT2, and Irs2 mRNA decreased, whereas Gcg, Gck, and Irs1 mRNA increased. Over time, during differentiation, insulin mRNA and protein expression increased, Gcg and Gck mRNA expression increased, Irs1 mRNA expression decreased and then increased, and Irs2 mRNA increased and then decreased (all P < 0.05). The expression of Dvl-2, LRP5, and GSK3β mRNA as well as the Dvl-2, GSK3β, and p-GSK3β proteins also increased (P < 0.05). Expression of TCF7L2 (6–10 d) and β-catenin mRNA as well as the β-catenin protein increased but not significantly (P > 0.05). Our results indicate that the Wnt signaling is activated during ADSC differentiation into islet β-cells, but there was no obvious enrichment of nonphosphorylated β-catenin protein.
format Online
Article
Text
id pubmed-5337876
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-53378762017-03-16 Effect of Wnt Signaling on the Differentiation of Islet β-Cells from Adipose-Derived Stem Cells Wang, Hefei Ren, Yu Hu, Xiao Ma, Min Wang, Xiao Liang, Hao Liu, Dongjun Biomed Res Int Research Article The Wnt signaling is critical for pancreatic development and islet function; however, its precise effects on the development and function of the β-cells remain controversial. Here we examined mRNA and protein expression of components of the Wnt signaling throughout the differentiation of islet β-cells from adipose-derived stem cells (ADSCs). After induction, ADSCs expressed markers of β-cells, including the insulin, PDX1, and glucagon genes, and the PDX1, CK19, nestin, insulin, and C-peptide proteins, indicating their successful differentiation. Compared with pancreatic adult stem cells (PASCs), the quantities of insulin, GLUT2, and Irs2 mRNA decreased, whereas Gcg, Gck, and Irs1 mRNA increased. Over time, during differentiation, insulin mRNA and protein expression increased, Gcg and Gck mRNA expression increased, Irs1 mRNA expression decreased and then increased, and Irs2 mRNA increased and then decreased (all P < 0.05). The expression of Dvl-2, LRP5, and GSK3β mRNA as well as the Dvl-2, GSK3β, and p-GSK3β proteins also increased (P < 0.05). Expression of TCF7L2 (6–10 d) and β-catenin mRNA as well as the β-catenin protein increased but not significantly (P > 0.05). Our results indicate that the Wnt signaling is activated during ADSC differentiation into islet β-cells, but there was no obvious enrichment of nonphosphorylated β-catenin protein. Hindawi Publishing Corporation 2017 2017-02-20 /pmc/articles/PMC5337876/ /pubmed/28303247 http://dx.doi.org/10.1155/2017/2501578 Text en Copyright © 2017 Hefei Wang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wang, Hefei
Ren, Yu
Hu, Xiao
Ma, Min
Wang, Xiao
Liang, Hao
Liu, Dongjun
Effect of Wnt Signaling on the Differentiation of Islet β-Cells from Adipose-Derived Stem Cells
title Effect of Wnt Signaling on the Differentiation of Islet β-Cells from Adipose-Derived Stem Cells
title_full Effect of Wnt Signaling on the Differentiation of Islet β-Cells from Adipose-Derived Stem Cells
title_fullStr Effect of Wnt Signaling on the Differentiation of Islet β-Cells from Adipose-Derived Stem Cells
title_full_unstemmed Effect of Wnt Signaling on the Differentiation of Islet β-Cells from Adipose-Derived Stem Cells
title_short Effect of Wnt Signaling on the Differentiation of Islet β-Cells from Adipose-Derived Stem Cells
title_sort effect of wnt signaling on the differentiation of islet β-cells from adipose-derived stem cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5337876/
https://www.ncbi.nlm.nih.gov/pubmed/28303247
http://dx.doi.org/10.1155/2017/2501578
work_keys_str_mv AT wanghefei effectofwntsignalingonthedifferentiationofisletbcellsfromadiposederivedstemcells
AT renyu effectofwntsignalingonthedifferentiationofisletbcellsfromadiposederivedstemcells
AT huxiao effectofwntsignalingonthedifferentiationofisletbcellsfromadiposederivedstemcells
AT mamin effectofwntsignalingonthedifferentiationofisletbcellsfromadiposederivedstemcells
AT wangxiao effectofwntsignalingonthedifferentiationofisletbcellsfromadiposederivedstemcells
AT lianghao effectofwntsignalingonthedifferentiationofisletbcellsfromadiposederivedstemcells
AT liudongjun effectofwntsignalingonthedifferentiationofisletbcellsfromadiposederivedstemcells