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Effect of Wnt Signaling on the Differentiation of Islet β-Cells from Adipose-Derived Stem Cells
The Wnt signaling is critical for pancreatic development and islet function; however, its precise effects on the development and function of the β-cells remain controversial. Here we examined mRNA and protein expression of components of the Wnt signaling throughout the differentiation of islet β-cel...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5337876/ https://www.ncbi.nlm.nih.gov/pubmed/28303247 http://dx.doi.org/10.1155/2017/2501578 |
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author | Wang, Hefei Ren, Yu Hu, Xiao Ma, Min Wang, Xiao Liang, Hao Liu, Dongjun |
author_facet | Wang, Hefei Ren, Yu Hu, Xiao Ma, Min Wang, Xiao Liang, Hao Liu, Dongjun |
author_sort | Wang, Hefei |
collection | PubMed |
description | The Wnt signaling is critical for pancreatic development and islet function; however, its precise effects on the development and function of the β-cells remain controversial. Here we examined mRNA and protein expression of components of the Wnt signaling throughout the differentiation of islet β-cells from adipose-derived stem cells (ADSCs). After induction, ADSCs expressed markers of β-cells, including the insulin, PDX1, and glucagon genes, and the PDX1, CK19, nestin, insulin, and C-peptide proteins, indicating their successful differentiation. Compared with pancreatic adult stem cells (PASCs), the quantities of insulin, GLUT2, and Irs2 mRNA decreased, whereas Gcg, Gck, and Irs1 mRNA increased. Over time, during differentiation, insulin mRNA and protein expression increased, Gcg and Gck mRNA expression increased, Irs1 mRNA expression decreased and then increased, and Irs2 mRNA increased and then decreased (all P < 0.05). The expression of Dvl-2, LRP5, and GSK3β mRNA as well as the Dvl-2, GSK3β, and p-GSK3β proteins also increased (P < 0.05). Expression of TCF7L2 (6–10 d) and β-catenin mRNA as well as the β-catenin protein increased but not significantly (P > 0.05). Our results indicate that the Wnt signaling is activated during ADSC differentiation into islet β-cells, but there was no obvious enrichment of nonphosphorylated β-catenin protein. |
format | Online Article Text |
id | pubmed-5337876 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-53378762017-03-16 Effect of Wnt Signaling on the Differentiation of Islet β-Cells from Adipose-Derived Stem Cells Wang, Hefei Ren, Yu Hu, Xiao Ma, Min Wang, Xiao Liang, Hao Liu, Dongjun Biomed Res Int Research Article The Wnt signaling is critical for pancreatic development and islet function; however, its precise effects on the development and function of the β-cells remain controversial. Here we examined mRNA and protein expression of components of the Wnt signaling throughout the differentiation of islet β-cells from adipose-derived stem cells (ADSCs). After induction, ADSCs expressed markers of β-cells, including the insulin, PDX1, and glucagon genes, and the PDX1, CK19, nestin, insulin, and C-peptide proteins, indicating their successful differentiation. Compared with pancreatic adult stem cells (PASCs), the quantities of insulin, GLUT2, and Irs2 mRNA decreased, whereas Gcg, Gck, and Irs1 mRNA increased. Over time, during differentiation, insulin mRNA and protein expression increased, Gcg and Gck mRNA expression increased, Irs1 mRNA expression decreased and then increased, and Irs2 mRNA increased and then decreased (all P < 0.05). The expression of Dvl-2, LRP5, and GSK3β mRNA as well as the Dvl-2, GSK3β, and p-GSK3β proteins also increased (P < 0.05). Expression of TCF7L2 (6–10 d) and β-catenin mRNA as well as the β-catenin protein increased but not significantly (P > 0.05). Our results indicate that the Wnt signaling is activated during ADSC differentiation into islet β-cells, but there was no obvious enrichment of nonphosphorylated β-catenin protein. Hindawi Publishing Corporation 2017 2017-02-20 /pmc/articles/PMC5337876/ /pubmed/28303247 http://dx.doi.org/10.1155/2017/2501578 Text en Copyright © 2017 Hefei Wang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wang, Hefei Ren, Yu Hu, Xiao Ma, Min Wang, Xiao Liang, Hao Liu, Dongjun Effect of Wnt Signaling on the Differentiation of Islet β-Cells from Adipose-Derived Stem Cells |
title | Effect of Wnt Signaling on the Differentiation of Islet β-Cells from Adipose-Derived Stem Cells |
title_full | Effect of Wnt Signaling on the Differentiation of Islet β-Cells from Adipose-Derived Stem Cells |
title_fullStr | Effect of Wnt Signaling on the Differentiation of Islet β-Cells from Adipose-Derived Stem Cells |
title_full_unstemmed | Effect of Wnt Signaling on the Differentiation of Islet β-Cells from Adipose-Derived Stem Cells |
title_short | Effect of Wnt Signaling on the Differentiation of Islet β-Cells from Adipose-Derived Stem Cells |
title_sort | effect of wnt signaling on the differentiation of islet β-cells from adipose-derived stem cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5337876/ https://www.ncbi.nlm.nih.gov/pubmed/28303247 http://dx.doi.org/10.1155/2017/2501578 |
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