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A standardized method for lectin microarray-based tissue glycome mapping
The significance of glycomic profiling has been highlighted by recent findings that structural changes of glycans are observed in many diseases, including cancer. Therefore, glycomic profiling of the whole body (glycome mapping) under different physiopathological states may contribute to the discove...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5337905/ https://www.ncbi.nlm.nih.gov/pubmed/28262709 http://dx.doi.org/10.1038/srep43560 |
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author | Zou, Xia Yoshida, Maki Nagai-Okatani, Chiaki Iwaki, Jun Matsuda, Atsushi Tan, Binbin Hagiwara, Kozue Sato, Takashi Itakura, Yoko Noro, Erika Kaji, Hiroyuki Toyoda, Masashi Zhang, Yan Narimatsu, Hisashi Kuno, Atsushi |
author_facet | Zou, Xia Yoshida, Maki Nagai-Okatani, Chiaki Iwaki, Jun Matsuda, Atsushi Tan, Binbin Hagiwara, Kozue Sato, Takashi Itakura, Yoko Noro, Erika Kaji, Hiroyuki Toyoda, Masashi Zhang, Yan Narimatsu, Hisashi Kuno, Atsushi |
author_sort | Zou, Xia |
collection | PubMed |
description | The significance of glycomic profiling has been highlighted by recent findings that structural changes of glycans are observed in many diseases, including cancer. Therefore, glycomic profiling of the whole body (glycome mapping) under different physiopathological states may contribute to the discovery of reliable biomarkers with disease-specific alterations. To achieve this, standardization of high-throughput and in-depth analysis of tissue glycome mapping is needed. However, this is a great challenge due to the lack of analytical methodology for glycans on small amounts of endogenous glycoproteins. Here, we established a standardized method of lectin-assisted tissue glycome mapping. Formalin-fixed, paraffin-embedded tissue sections were prepared from brain, liver, kidney, spleen, and testis of two C57BL/6J mice. In total, 190 size-adjusted fragments with different morphology were serially collected from each tissue by laser microdissection and subjected to lectin microarray analysis. The results and subsequent histochemical analysis with selected lectins were highly consistent with previous reports of mass spectrometry-based N- and/or O-glycome analyses and histochemistry. This is the first report to look at both N- and O-glycome profiles of various regions within tissue sections of five different organs. This simple and reproducible mapping approach is also applicable to various disease model mice to facilitate disease-related biomarker discovery. |
format | Online Article Text |
id | pubmed-5337905 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-53379052017-03-08 A standardized method for lectin microarray-based tissue glycome mapping Zou, Xia Yoshida, Maki Nagai-Okatani, Chiaki Iwaki, Jun Matsuda, Atsushi Tan, Binbin Hagiwara, Kozue Sato, Takashi Itakura, Yoko Noro, Erika Kaji, Hiroyuki Toyoda, Masashi Zhang, Yan Narimatsu, Hisashi Kuno, Atsushi Sci Rep Article The significance of glycomic profiling has been highlighted by recent findings that structural changes of glycans are observed in many diseases, including cancer. Therefore, glycomic profiling of the whole body (glycome mapping) under different physiopathological states may contribute to the discovery of reliable biomarkers with disease-specific alterations. To achieve this, standardization of high-throughput and in-depth analysis of tissue glycome mapping is needed. However, this is a great challenge due to the lack of analytical methodology for glycans on small amounts of endogenous glycoproteins. Here, we established a standardized method of lectin-assisted tissue glycome mapping. Formalin-fixed, paraffin-embedded tissue sections were prepared from brain, liver, kidney, spleen, and testis of two C57BL/6J mice. In total, 190 size-adjusted fragments with different morphology were serially collected from each tissue by laser microdissection and subjected to lectin microarray analysis. The results and subsequent histochemical analysis with selected lectins were highly consistent with previous reports of mass spectrometry-based N- and/or O-glycome analyses and histochemistry. This is the first report to look at both N- and O-glycome profiles of various regions within tissue sections of five different organs. This simple and reproducible mapping approach is also applicable to various disease model mice to facilitate disease-related biomarker discovery. Nature Publishing Group 2017-03-06 /pmc/articles/PMC5337905/ /pubmed/28262709 http://dx.doi.org/10.1038/srep43560 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Zou, Xia Yoshida, Maki Nagai-Okatani, Chiaki Iwaki, Jun Matsuda, Atsushi Tan, Binbin Hagiwara, Kozue Sato, Takashi Itakura, Yoko Noro, Erika Kaji, Hiroyuki Toyoda, Masashi Zhang, Yan Narimatsu, Hisashi Kuno, Atsushi A standardized method for lectin microarray-based tissue glycome mapping |
title | A standardized method for lectin microarray-based tissue glycome mapping |
title_full | A standardized method for lectin microarray-based tissue glycome mapping |
title_fullStr | A standardized method for lectin microarray-based tissue glycome mapping |
title_full_unstemmed | A standardized method for lectin microarray-based tissue glycome mapping |
title_short | A standardized method for lectin microarray-based tissue glycome mapping |
title_sort | standardized method for lectin microarray-based tissue glycome mapping |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5337905/ https://www.ncbi.nlm.nih.gov/pubmed/28262709 http://dx.doi.org/10.1038/srep43560 |
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