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The RNAi pathway plays a small part in Wolbachia-mediated blocking of dengue virus in mosquito cells
Wolbachia pipientis is an insect endosymbiont known to limit the replication of viruses including dengue and Zika in their primary mosquito vector, Aedes aegypti. Wolbachia is being released into mosquito populations globally in a bid to control the diseases caused by these viruses. It is theorized...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5338330/ https://www.ncbi.nlm.nih.gov/pubmed/28262718 http://dx.doi.org/10.1038/srep43847 |
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author | Terradas, Gerard Joubert, D. Albert McGraw, Elizabeth A. |
author_facet | Terradas, Gerard Joubert, D. Albert McGraw, Elizabeth A. |
author_sort | Terradas, Gerard |
collection | PubMed |
description | Wolbachia pipientis is an insect endosymbiont known to limit the replication of viruses including dengue and Zika in their primary mosquito vector, Aedes aegypti. Wolbachia is being released into mosquito populations globally in a bid to control the diseases caused by these viruses. It is theorized that Wolbachia’s priming of the insect immune system may confer protection against subsequent viral infection. Other hypotheses posit a role for competition between Wolbachia and viruses for host cellular resources. Using an A. aegypti cell line infected with Wolbachia, we tested the effects of targeting siRNAs against the major innate immune pathways on dengue virus loads. We show that while Wolbachia infection induces genes in the Toll, JAK/STAT and RNAi pathways, only reduced expression of RNAi leads to a rebound of dengue virus loads in Wolbachia-infected cells. The magnitude of the effect explained less than 10% of the total DENV load, demonstrating that blocking must be dependent on other factors in addition to the expression of RNAi. The findings bode well for the long-term stability of blocking given that immunity gene expression would likely be highly plastic and susceptible to rapid evolution. |
format | Online Article Text |
id | pubmed-5338330 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-53383302017-03-08 The RNAi pathway plays a small part in Wolbachia-mediated blocking of dengue virus in mosquito cells Terradas, Gerard Joubert, D. Albert McGraw, Elizabeth A. Sci Rep Article Wolbachia pipientis is an insect endosymbiont known to limit the replication of viruses including dengue and Zika in their primary mosquito vector, Aedes aegypti. Wolbachia is being released into mosquito populations globally in a bid to control the diseases caused by these viruses. It is theorized that Wolbachia’s priming of the insect immune system may confer protection against subsequent viral infection. Other hypotheses posit a role for competition between Wolbachia and viruses for host cellular resources. Using an A. aegypti cell line infected with Wolbachia, we tested the effects of targeting siRNAs against the major innate immune pathways on dengue virus loads. We show that while Wolbachia infection induces genes in the Toll, JAK/STAT and RNAi pathways, only reduced expression of RNAi leads to a rebound of dengue virus loads in Wolbachia-infected cells. The magnitude of the effect explained less than 10% of the total DENV load, demonstrating that blocking must be dependent on other factors in addition to the expression of RNAi. The findings bode well for the long-term stability of blocking given that immunity gene expression would likely be highly plastic and susceptible to rapid evolution. Nature Publishing Group 2017-03-06 /pmc/articles/PMC5338330/ /pubmed/28262718 http://dx.doi.org/10.1038/srep43847 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Terradas, Gerard Joubert, D. Albert McGraw, Elizabeth A. The RNAi pathway plays a small part in Wolbachia-mediated blocking of dengue virus in mosquito cells |
title | The RNAi pathway plays a small part in Wolbachia-mediated blocking of dengue virus in mosquito cells |
title_full | The RNAi pathway plays a small part in Wolbachia-mediated blocking of dengue virus in mosquito cells |
title_fullStr | The RNAi pathway plays a small part in Wolbachia-mediated blocking of dengue virus in mosquito cells |
title_full_unstemmed | The RNAi pathway plays a small part in Wolbachia-mediated blocking of dengue virus in mosquito cells |
title_short | The RNAi pathway plays a small part in Wolbachia-mediated blocking of dengue virus in mosquito cells |
title_sort | rnai pathway plays a small part in wolbachia-mediated blocking of dengue virus in mosquito cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5338330/ https://www.ncbi.nlm.nih.gov/pubmed/28262718 http://dx.doi.org/10.1038/srep43847 |
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