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Methylation analysis and HPV genotyping of self-collected cervical samples from women not responding to screening invitation and review of the literature
AIM OF THE STUDY: To assess the feasibility of partial HPV genotyping and methylation analysis of CADM1, MAL, and miR124-2 genes as triage tests in assaying self-collected cervical samples positive for high-risk HPV on primary screening, and to review the literature regarding host cellular gene meth...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5338782/ https://www.ncbi.nlm.nih.gov/pubmed/28263992 http://dx.doi.org/10.1371/journal.pone.0172226 |
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author | Del Mistro, Annarosa Frayle, Helena Rizzi, Martina Fantin, Gianpiero Ferro, Antonio Angeletti, Paolo Matteo Giorgi Rossi, Paolo Altobelli, Emma |
author_facet | Del Mistro, Annarosa Frayle, Helena Rizzi, Martina Fantin, Gianpiero Ferro, Antonio Angeletti, Paolo Matteo Giorgi Rossi, Paolo Altobelli, Emma |
author_sort | Del Mistro, Annarosa |
collection | PubMed |
description | AIM OF THE STUDY: To assess the feasibility of partial HPV genotyping and methylation analysis of CADM1, MAL, and miR124-2 genes as triage tests in assaying self-collected cervical samples positive for high-risk HPV on primary screening, and to review the literature regarding host cellular gene methylation analysis of self-collected cervical samples. MATERIAL AND METHODS: Women residing in North-East Italy who had failed to respond to the invitation to participate in an organized population-based program were invited to provide a self-sample. Their stored baseline (self-collected) and follow-up (clinician-collected) cervical samples were included in the study. DNA was extracted from HPV-positive (Qiagen’s Hybrid Capture 2, HC2) samples. Partial genotyping with separate detection of HPV types 16 and 18 was performed with a hybrid capture-based method and a quantitative PCR assay. Methylation was assayed with a quantitative methylation-specific PCR. RESULTS: High-risk HPV infection was detected in 48% of baseline and 71% of follow-up HC2-positive samples. Methylation was demonstrated respectively in 15% and 23.5% of baseline and follow-up samples and chiefly involved a single gene (miR124-2). Invalid quantitative PCR results were recorded in 5% of self-collected samples. The specificity of miR124-1, MAL, and CADM1 methylation was 84%, 94%, and 98%, respectively, and the specificity of the three markers combined was 84%. Sensitivity was not estimated due to the lack of CIN2+ samples. The systematic review showed that different methylation assays yield different accuracy values. CONCLUSION: Self-collected samples are suitable for methylation assays included in reflex triage testing. The reproducibility and accuracy of the methylation tests described in the literature should be improved. |
format | Online Article Text |
id | pubmed-5338782 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-53387822017-03-10 Methylation analysis and HPV genotyping of self-collected cervical samples from women not responding to screening invitation and review of the literature Del Mistro, Annarosa Frayle, Helena Rizzi, Martina Fantin, Gianpiero Ferro, Antonio Angeletti, Paolo Matteo Giorgi Rossi, Paolo Altobelli, Emma PLoS One Research Article AIM OF THE STUDY: To assess the feasibility of partial HPV genotyping and methylation analysis of CADM1, MAL, and miR124-2 genes as triage tests in assaying self-collected cervical samples positive for high-risk HPV on primary screening, and to review the literature regarding host cellular gene methylation analysis of self-collected cervical samples. MATERIAL AND METHODS: Women residing in North-East Italy who had failed to respond to the invitation to participate in an organized population-based program were invited to provide a self-sample. Their stored baseline (self-collected) and follow-up (clinician-collected) cervical samples were included in the study. DNA was extracted from HPV-positive (Qiagen’s Hybrid Capture 2, HC2) samples. Partial genotyping with separate detection of HPV types 16 and 18 was performed with a hybrid capture-based method and a quantitative PCR assay. Methylation was assayed with a quantitative methylation-specific PCR. RESULTS: High-risk HPV infection was detected in 48% of baseline and 71% of follow-up HC2-positive samples. Methylation was demonstrated respectively in 15% and 23.5% of baseline and follow-up samples and chiefly involved a single gene (miR124-2). Invalid quantitative PCR results were recorded in 5% of self-collected samples. The specificity of miR124-1, MAL, and CADM1 methylation was 84%, 94%, and 98%, respectively, and the specificity of the three markers combined was 84%. Sensitivity was not estimated due to the lack of CIN2+ samples. The systematic review showed that different methylation assays yield different accuracy values. CONCLUSION: Self-collected samples are suitable for methylation assays included in reflex triage testing. The reproducibility and accuracy of the methylation tests described in the literature should be improved. Public Library of Science 2017-03-06 /pmc/articles/PMC5338782/ /pubmed/28263992 http://dx.doi.org/10.1371/journal.pone.0172226 Text en © 2017 Del Mistro et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Del Mistro, Annarosa Frayle, Helena Rizzi, Martina Fantin, Gianpiero Ferro, Antonio Angeletti, Paolo Matteo Giorgi Rossi, Paolo Altobelli, Emma Methylation analysis and HPV genotyping of self-collected cervical samples from women not responding to screening invitation and review of the literature |
title | Methylation analysis and HPV genotyping of self-collected cervical samples from women not responding to screening invitation and review of the literature |
title_full | Methylation analysis and HPV genotyping of self-collected cervical samples from women not responding to screening invitation and review of the literature |
title_fullStr | Methylation analysis and HPV genotyping of self-collected cervical samples from women not responding to screening invitation and review of the literature |
title_full_unstemmed | Methylation analysis and HPV genotyping of self-collected cervical samples from women not responding to screening invitation and review of the literature |
title_short | Methylation analysis and HPV genotyping of self-collected cervical samples from women not responding to screening invitation and review of the literature |
title_sort | methylation analysis and hpv genotyping of self-collected cervical samples from women not responding to screening invitation and review of the literature |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5338782/ https://www.ncbi.nlm.nih.gov/pubmed/28263992 http://dx.doi.org/10.1371/journal.pone.0172226 |
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