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Different Proteome Profiles between Male and Female Populus cathayana Exposed to UV-B Radiation

With increasing altitude, solar UV-B radiation is enhanced. Based on the phenomenon of male-biased sex ratio of Populus cathayana Rehder in high altitude alpine area, we hypothesized that males have a faster and more sophisticated responsive mechanism to high UV-B radiation than that of females. Our...

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Autores principales: Zhang, Yunxiang, Feng, Lihua, Jiang, Hao, Zhang, Yuanbin, Zhang, Sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5339244/
https://www.ncbi.nlm.nih.gov/pubmed/28326097
http://dx.doi.org/10.3389/fpls.2017.00320
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author Zhang, Yunxiang
Feng, Lihua
Jiang, Hao
Zhang, Yuanbin
Zhang, Sheng
author_facet Zhang, Yunxiang
Feng, Lihua
Jiang, Hao
Zhang, Yuanbin
Zhang, Sheng
author_sort Zhang, Yunxiang
collection PubMed
description With increasing altitude, solar UV-B radiation is enhanced. Based on the phenomenon of male-biased sex ratio of Populus cathayana Rehder in high altitude alpine area, we hypothesized that males have a faster and more sophisticated responsive mechanism to high UV-B radiation than that of females. Our previous studies have shown sexually different responses to high UV-B radiation were existed in P. cathayana at the morphological, physiological, and transcriptomic levels. However, the responses at the proteomic level remain unclear. In this study, an isobaric tag for relative and absolute quantification (iTRAQ)-based quantitative proteome analysis was performed in P. cathayana females and males. A total of 2,405 proteins were identified, with 331 proteins defined as differentially expressed proteins (DEPs). Among of these, 79 and 138 DEPs were decreased and 47 and 107 DEPs were increased under high solar UV-B radiation in females and males, respectively. A bioinformatics analysis categorized the common responsive proteins in the sexes as related to carbohydrate and energy metabolism, translation/transcription/post-transcriptional modification, photosynthesis, and redox reactions. The responsive proteins that showed differences in sex were mainly those involved in amino acid metabolism, stress response, and translation/transcription/post-transcriptional modification. This study provides proteomic profiles that poplars responding to solar UV-B radiation, and it also provides new insights into differentially sex-related responses to UV-B radiation.
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spelling pubmed-53392442017-03-21 Different Proteome Profiles between Male and Female Populus cathayana Exposed to UV-B Radiation Zhang, Yunxiang Feng, Lihua Jiang, Hao Zhang, Yuanbin Zhang, Sheng Front Plant Sci Plant Science With increasing altitude, solar UV-B radiation is enhanced. Based on the phenomenon of male-biased sex ratio of Populus cathayana Rehder in high altitude alpine area, we hypothesized that males have a faster and more sophisticated responsive mechanism to high UV-B radiation than that of females. Our previous studies have shown sexually different responses to high UV-B radiation were existed in P. cathayana at the morphological, physiological, and transcriptomic levels. However, the responses at the proteomic level remain unclear. In this study, an isobaric tag for relative and absolute quantification (iTRAQ)-based quantitative proteome analysis was performed in P. cathayana females and males. A total of 2,405 proteins were identified, with 331 proteins defined as differentially expressed proteins (DEPs). Among of these, 79 and 138 DEPs were decreased and 47 and 107 DEPs were increased under high solar UV-B radiation in females and males, respectively. A bioinformatics analysis categorized the common responsive proteins in the sexes as related to carbohydrate and energy metabolism, translation/transcription/post-transcriptional modification, photosynthesis, and redox reactions. The responsive proteins that showed differences in sex were mainly those involved in amino acid metabolism, stress response, and translation/transcription/post-transcriptional modification. This study provides proteomic profiles that poplars responding to solar UV-B radiation, and it also provides new insights into differentially sex-related responses to UV-B radiation. Frontiers Media S.A. 2017-03-07 /pmc/articles/PMC5339244/ /pubmed/28326097 http://dx.doi.org/10.3389/fpls.2017.00320 Text en Copyright © 2017 Zhang, Feng, Jiang, Zhang and Zhang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Zhang, Yunxiang
Feng, Lihua
Jiang, Hao
Zhang, Yuanbin
Zhang, Sheng
Different Proteome Profiles between Male and Female Populus cathayana Exposed to UV-B Radiation
title Different Proteome Profiles between Male and Female Populus cathayana Exposed to UV-B Radiation
title_full Different Proteome Profiles between Male and Female Populus cathayana Exposed to UV-B Radiation
title_fullStr Different Proteome Profiles between Male and Female Populus cathayana Exposed to UV-B Radiation
title_full_unstemmed Different Proteome Profiles between Male and Female Populus cathayana Exposed to UV-B Radiation
title_short Different Proteome Profiles between Male and Female Populus cathayana Exposed to UV-B Radiation
title_sort different proteome profiles between male and female populus cathayana exposed to uv-b radiation
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5339244/
https://www.ncbi.nlm.nih.gov/pubmed/28326097
http://dx.doi.org/10.3389/fpls.2017.00320
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