Negative Regulation of Violacein Biosynthesis in Chromobacterium violaceum

In Chromobacteium violaceum, the purple pigment violacein is under positive regulation by the N-acylhomoserine lactone CviI/R quorum sensing system and negative regulation by an uncharacterized putative repressor. In this study we report that the biosynthesis of violacein is negatively controlled by...

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Autores principales: Devescovi, Giulia, Kojic, Milan, Covaceuszach, Sonia, Cámara, Miguel, Williams, Paul, Bertani, Iris, Subramoni, Sujatha, Venturi, Vittorio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5339254/
https://www.ncbi.nlm.nih.gov/pubmed/28326068
http://dx.doi.org/10.3389/fmicb.2017.00349
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author Devescovi, Giulia
Kojic, Milan
Covaceuszach, Sonia
Cámara, Miguel
Williams, Paul
Bertani, Iris
Subramoni, Sujatha
Venturi, Vittorio
author_facet Devescovi, Giulia
Kojic, Milan
Covaceuszach, Sonia
Cámara, Miguel
Williams, Paul
Bertani, Iris
Subramoni, Sujatha
Venturi, Vittorio
author_sort Devescovi, Giulia
collection PubMed
description In Chromobacteium violaceum, the purple pigment violacein is under positive regulation by the N-acylhomoserine lactone CviI/R quorum sensing system and negative regulation by an uncharacterized putative repressor. In this study we report that the biosynthesis of violacein is negatively controlled by a novel repressor protein, VioS. The violacein operon is regulated negatively by VioS and positively by the CviI/R system in both C. violaceum and in a heterologous Escherichia coli genetic background. VioS does not regulate the CviI/R system and apart from violacein, VioS, and quorum sensing regulate other phenotypes antagonistically. Quorum sensing regulated phenotypes in C. violaceum are therefore further regulated providing an additional level of control.
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spelling pubmed-53392542017-03-21 Negative Regulation of Violacein Biosynthesis in Chromobacterium violaceum Devescovi, Giulia Kojic, Milan Covaceuszach, Sonia Cámara, Miguel Williams, Paul Bertani, Iris Subramoni, Sujatha Venturi, Vittorio Front Microbiol Microbiology In Chromobacteium violaceum, the purple pigment violacein is under positive regulation by the N-acylhomoserine lactone CviI/R quorum sensing system and negative regulation by an uncharacterized putative repressor. In this study we report that the biosynthesis of violacein is negatively controlled by a novel repressor protein, VioS. The violacein operon is regulated negatively by VioS and positively by the CviI/R system in both C. violaceum and in a heterologous Escherichia coli genetic background. VioS does not regulate the CviI/R system and apart from violacein, VioS, and quorum sensing regulate other phenotypes antagonistically. Quorum sensing regulated phenotypes in C. violaceum are therefore further regulated providing an additional level of control. Frontiers Media S.A. 2017-03-07 /pmc/articles/PMC5339254/ /pubmed/28326068 http://dx.doi.org/10.3389/fmicb.2017.00349 Text en Copyright © 2017 Devescovi, Kojic, Covaceuszach, Cámara, Williams, Bertani, Subramoni and Venturi. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Devescovi, Giulia
Kojic, Milan
Covaceuszach, Sonia
Cámara, Miguel
Williams, Paul
Bertani, Iris
Subramoni, Sujatha
Venturi, Vittorio
Negative Regulation of Violacein Biosynthesis in Chromobacterium violaceum
title Negative Regulation of Violacein Biosynthesis in Chromobacterium violaceum
title_full Negative Regulation of Violacein Biosynthesis in Chromobacterium violaceum
title_fullStr Negative Regulation of Violacein Biosynthesis in Chromobacterium violaceum
title_full_unstemmed Negative Regulation of Violacein Biosynthesis in Chromobacterium violaceum
title_short Negative Regulation of Violacein Biosynthesis in Chromobacterium violaceum
title_sort negative regulation of violacein biosynthesis in chromobacterium violaceum
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5339254/
https://www.ncbi.nlm.nih.gov/pubmed/28326068
http://dx.doi.org/10.3389/fmicb.2017.00349
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