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Evaluation of the expression of VIII factor and VEGF in the regeneration of non-vital teeth in dogs using propolis
OBJECTIVE(S): The purpose of the present study was the immunohistochemical evaluation of VEGF and VII factors in dog’s teeth pulp revascularized with MTA and propolis. MATERIALS AND METHODS: 144 mature and immature two rooted dog’s premolar canals were selected. Pulp necrosis and infection were esta...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mashhad University of Medical Sciences
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5339658/ https://www.ncbi.nlm.nih.gov/pubmed/28293394 http://dx.doi.org/10.22038/ijbms.2017.8243 |
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author | Zarei, Mina Jafarian, Amir Hossein Harandi, Azadeh Javidi, Maryam Gharechahi, Maryam |
author_facet | Zarei, Mina Jafarian, Amir Hossein Harandi, Azadeh Javidi, Maryam Gharechahi, Maryam |
author_sort | Zarei, Mina |
collection | PubMed |
description | OBJECTIVE(S): The purpose of the present study was the immunohistochemical evaluation of VEGF and VII factors in dog’s teeth pulp revascularized with MTA and propolis. MATERIALS AND METHODS: 144 mature and immature two rooted dog’s premolar canals were selected. Pulp necrosis and infection were established after 2 weeks and the disinfection of the canals was done with copious NaOCl irrigation and triantibiotic mixture (ciprofloxacin, metronidazole, and minocycline) for 3 weeks. Subsequently, the blood clot was evoked in the canal by periapical tissue irritation with a k-file. The samples were randomly divided into 6 experimental groups: propolis (groups 1, 2), MTA (groups 3, 4), and parafilm (groups 5, 6) in immature and mature teeth. The animals were sacrificed and samples were prepared for immunohistochemical evaluation of VEGF and the VIII factor. RESULTS: Tissue regeneration was seen in 64.5% of MTA, 38% of propolis, and 0% of parafilm group samples. Expression of VEGF and VIII factor in the propolis group was more than the MTA group and it showed a reduction after 3 months in comparison to 1 month. VEGF and VIII factor were seen in stromal cells in addition to endothelial vessel cells. Overall, expression of angiogenic factors was more in the open apex teeth compared to close apex ones. CONCLUSION: According to the results of this study, propolis can induce the expression of VEGF and VIII factor in infected mature and immature dog’s teeth and is a suitable biomaterial for the revascularization technique. |
format | Online Article Text |
id | pubmed-5339658 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Mashhad University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-53396582017-03-14 Evaluation of the expression of VIII factor and VEGF in the regeneration of non-vital teeth in dogs using propolis Zarei, Mina Jafarian, Amir Hossein Harandi, Azadeh Javidi, Maryam Gharechahi, Maryam Iran J Basic Med Sci Original Article OBJECTIVE(S): The purpose of the present study was the immunohistochemical evaluation of VEGF and VII factors in dog’s teeth pulp revascularized with MTA and propolis. MATERIALS AND METHODS: 144 mature and immature two rooted dog’s premolar canals were selected. Pulp necrosis and infection were established after 2 weeks and the disinfection of the canals was done with copious NaOCl irrigation and triantibiotic mixture (ciprofloxacin, metronidazole, and minocycline) for 3 weeks. Subsequently, the blood clot was evoked in the canal by periapical tissue irritation with a k-file. The samples were randomly divided into 6 experimental groups: propolis (groups 1, 2), MTA (groups 3, 4), and parafilm (groups 5, 6) in immature and mature teeth. The animals were sacrificed and samples were prepared for immunohistochemical evaluation of VEGF and the VIII factor. RESULTS: Tissue regeneration was seen in 64.5% of MTA, 38% of propolis, and 0% of parafilm group samples. Expression of VEGF and VIII factor in the propolis group was more than the MTA group and it showed a reduction after 3 months in comparison to 1 month. VEGF and VIII factor were seen in stromal cells in addition to endothelial vessel cells. Overall, expression of angiogenic factors was more in the open apex teeth compared to close apex ones. CONCLUSION: According to the results of this study, propolis can induce the expression of VEGF and VIII factor in infected mature and immature dog’s teeth and is a suitable biomaterial for the revascularization technique. Mashhad University of Medical Sciences 2017-02 /pmc/articles/PMC5339658/ /pubmed/28293394 http://dx.doi.org/10.22038/ijbms.2017.8243 Text en Copyright: © Iranian Journal of Basic Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Zarei, Mina Jafarian, Amir Hossein Harandi, Azadeh Javidi, Maryam Gharechahi, Maryam Evaluation of the expression of VIII factor and VEGF in the regeneration of non-vital teeth in dogs using propolis |
title | Evaluation of the expression of VIII factor and VEGF in the regeneration of non-vital teeth in dogs using propolis |
title_full | Evaluation of the expression of VIII factor and VEGF in the regeneration of non-vital teeth in dogs using propolis |
title_fullStr | Evaluation of the expression of VIII factor and VEGF in the regeneration of non-vital teeth in dogs using propolis |
title_full_unstemmed | Evaluation of the expression of VIII factor and VEGF in the regeneration of non-vital teeth in dogs using propolis |
title_short | Evaluation of the expression of VIII factor and VEGF in the regeneration of non-vital teeth in dogs using propolis |
title_sort | evaluation of the expression of viii factor and vegf in the regeneration of non-vital teeth in dogs using propolis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5339658/ https://www.ncbi.nlm.nih.gov/pubmed/28293394 http://dx.doi.org/10.22038/ijbms.2017.8243 |
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