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Evaluation of the expression of VIII factor and VEGF in the regeneration of non-vital teeth in dogs using propolis

OBJECTIVE(S): The purpose of the present study was the immunohistochemical evaluation of VEGF and VII factors in dog’s teeth pulp revascularized with MTA and propolis. MATERIALS AND METHODS: 144 mature and immature two rooted dog’s premolar canals were selected. Pulp necrosis and infection were esta...

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Autores principales: Zarei, Mina, Jafarian, Amir Hossein, Harandi, Azadeh, Javidi, Maryam, Gharechahi, Maryam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5339658/
https://www.ncbi.nlm.nih.gov/pubmed/28293394
http://dx.doi.org/10.22038/ijbms.2017.8243
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author Zarei, Mina
Jafarian, Amir Hossein
Harandi, Azadeh
Javidi, Maryam
Gharechahi, Maryam
author_facet Zarei, Mina
Jafarian, Amir Hossein
Harandi, Azadeh
Javidi, Maryam
Gharechahi, Maryam
author_sort Zarei, Mina
collection PubMed
description OBJECTIVE(S): The purpose of the present study was the immunohistochemical evaluation of VEGF and VII factors in dog’s teeth pulp revascularized with MTA and propolis. MATERIALS AND METHODS: 144 mature and immature two rooted dog’s premolar canals were selected. Pulp necrosis and infection were established after 2 weeks and the disinfection of the canals was done with copious NaOCl irrigation and triantibiotic mixture (ciprofloxacin, metronidazole, and minocycline) for 3 weeks. Subsequently, the blood clot was evoked in the canal by periapical tissue irritation with a k-file. The samples were randomly divided into 6 experimental groups: propolis (groups 1, 2), MTA (groups 3, 4), and parafilm (groups 5, 6) in immature and mature teeth. The animals were sacrificed and samples were prepared for immunohistochemical evaluation of VEGF and the VIII factor. RESULTS: Tissue regeneration was seen in 64.5% of MTA, 38% of propolis, and 0% of parafilm group samples. Expression of VEGF and VIII factor in the propolis group was more than the MTA group and it showed a reduction after 3 months in comparison to 1 month. VEGF and VIII factor were seen in stromal cells in addition to endothelial vessel cells. Overall, expression of angiogenic factors was more in the open apex teeth compared to close apex ones. CONCLUSION: According to the results of this study, propolis can induce the expression of VEGF and VIII factor in infected mature and immature dog’s teeth and is a suitable biomaterial for the revascularization technique.
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spelling pubmed-53396582017-03-14 Evaluation of the expression of VIII factor and VEGF in the regeneration of non-vital teeth in dogs using propolis Zarei, Mina Jafarian, Amir Hossein Harandi, Azadeh Javidi, Maryam Gharechahi, Maryam Iran J Basic Med Sci Original Article OBJECTIVE(S): The purpose of the present study was the immunohistochemical evaluation of VEGF and VII factors in dog’s teeth pulp revascularized with MTA and propolis. MATERIALS AND METHODS: 144 mature and immature two rooted dog’s premolar canals were selected. Pulp necrosis and infection were established after 2 weeks and the disinfection of the canals was done with copious NaOCl irrigation and triantibiotic mixture (ciprofloxacin, metronidazole, and minocycline) for 3 weeks. Subsequently, the blood clot was evoked in the canal by periapical tissue irritation with a k-file. The samples were randomly divided into 6 experimental groups: propolis (groups 1, 2), MTA (groups 3, 4), and parafilm (groups 5, 6) in immature and mature teeth. The animals were sacrificed and samples were prepared for immunohistochemical evaluation of VEGF and the VIII factor. RESULTS: Tissue regeneration was seen in 64.5% of MTA, 38% of propolis, and 0% of parafilm group samples. Expression of VEGF and VIII factor in the propolis group was more than the MTA group and it showed a reduction after 3 months in comparison to 1 month. VEGF and VIII factor were seen in stromal cells in addition to endothelial vessel cells. Overall, expression of angiogenic factors was more in the open apex teeth compared to close apex ones. CONCLUSION: According to the results of this study, propolis can induce the expression of VEGF and VIII factor in infected mature and immature dog’s teeth and is a suitable biomaterial for the revascularization technique. Mashhad University of Medical Sciences 2017-02 /pmc/articles/PMC5339658/ /pubmed/28293394 http://dx.doi.org/10.22038/ijbms.2017.8243 Text en Copyright: © Iranian Journal of Basic Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Zarei, Mina
Jafarian, Amir Hossein
Harandi, Azadeh
Javidi, Maryam
Gharechahi, Maryam
Evaluation of the expression of VIII factor and VEGF in the regeneration of non-vital teeth in dogs using propolis
title Evaluation of the expression of VIII factor and VEGF in the regeneration of non-vital teeth in dogs using propolis
title_full Evaluation of the expression of VIII factor and VEGF in the regeneration of non-vital teeth in dogs using propolis
title_fullStr Evaluation of the expression of VIII factor and VEGF in the regeneration of non-vital teeth in dogs using propolis
title_full_unstemmed Evaluation of the expression of VIII factor and VEGF in the regeneration of non-vital teeth in dogs using propolis
title_short Evaluation of the expression of VIII factor and VEGF in the regeneration of non-vital teeth in dogs using propolis
title_sort evaluation of the expression of viii factor and vegf in the regeneration of non-vital teeth in dogs using propolis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5339658/
https://www.ncbi.nlm.nih.gov/pubmed/28293394
http://dx.doi.org/10.22038/ijbms.2017.8243
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