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Nicotine-induced damages in testicular tissue of rats; evidences for bcl-2, p53 and caspase-3 expression
OBJECTIVE(S): Present study was performed in order to uncover new aspects for nicotine-induced damages on spermatogenesis cell lineage. MATERIALS AND METHODS: For this purpose, 36 mature male Wistar rats were divided into three groups as; control-sham (0.2 ml, saline normal, IP), low dose (0.2 mg/kg...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mashhad University of Medical Sciences
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5339662/ https://www.ncbi.nlm.nih.gov/pubmed/28293398 http://dx.doi.org/10.22038/ijbms.2017.8249 |
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author | Mosadegh, Maryam Hasanzadeh, Shapour Razi, Mazdak |
author_facet | Mosadegh, Maryam Hasanzadeh, Shapour Razi, Mazdak |
author_sort | Mosadegh, Maryam |
collection | PubMed |
description | OBJECTIVE(S): Present study was performed in order to uncover new aspects for nicotine-induced damages on spermatogenesis cell lineage. MATERIALS AND METHODS: For this purpose, 36 mature male Wistar rats were divided into three groups as; control-sham (0.2 ml, saline normal, IP), low dose (0.2 mg/kg BW(-1), IP) nicotine-received and high dose (0.4 mg/kg BW(-1), IP) nicotine-received groups. Following 7 weeks, the expression of bcl-2, p53 and caspase-3 at mRNA and protein levels were investigated by using reverse-transcriptase PCR (RT-PCR) and immunohistochemical (IHC) analyses, respectively. Moreover, the serum level of FSH, LH and testosterone were evaluated. Finally, the mRNA damage was analyzed by using special fluorescent staining. RESULTS: Nicotine, at both dose levels, decreased tubular differentiation, spermiogenesis and repopulation indices and enhanced cellular depletion. Animals in nicotine-received groups exhibited a significant (P<0.05) reduction at mRNA and protein levels of bcl-2. More analyses revealed a remarkable (P<0.05) enhancement in expression of p53 and caspase-3 in comparison to control-sham animals. Finally, nicotine resulted in a significant (P<0.05) reduction in serum level of testosterone and elevated mRNA damage. CONCLUSION: Our data showed that, nicotine by suppressing the testosterone biosynthesis, reducing mRNA and protein levels of bcl-2 and up regulating the p53 and caspase-3 mRNA and protein levels adversely affects the spermatogenesis and results in cellular depletion. |
format | Online Article Text |
id | pubmed-5339662 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Mashhad University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-53396622017-03-14 Nicotine-induced damages in testicular tissue of rats; evidences for bcl-2, p53 and caspase-3 expression Mosadegh, Maryam Hasanzadeh, Shapour Razi, Mazdak Iran J Basic Med Sci Original Article OBJECTIVE(S): Present study was performed in order to uncover new aspects for nicotine-induced damages on spermatogenesis cell lineage. MATERIALS AND METHODS: For this purpose, 36 mature male Wistar rats were divided into three groups as; control-sham (0.2 ml, saline normal, IP), low dose (0.2 mg/kg BW(-1), IP) nicotine-received and high dose (0.4 mg/kg BW(-1), IP) nicotine-received groups. Following 7 weeks, the expression of bcl-2, p53 and caspase-3 at mRNA and protein levels were investigated by using reverse-transcriptase PCR (RT-PCR) and immunohistochemical (IHC) analyses, respectively. Moreover, the serum level of FSH, LH and testosterone were evaluated. Finally, the mRNA damage was analyzed by using special fluorescent staining. RESULTS: Nicotine, at both dose levels, decreased tubular differentiation, spermiogenesis and repopulation indices and enhanced cellular depletion. Animals in nicotine-received groups exhibited a significant (P<0.05) reduction at mRNA and protein levels of bcl-2. More analyses revealed a remarkable (P<0.05) enhancement in expression of p53 and caspase-3 in comparison to control-sham animals. Finally, nicotine resulted in a significant (P<0.05) reduction in serum level of testosterone and elevated mRNA damage. CONCLUSION: Our data showed that, nicotine by suppressing the testosterone biosynthesis, reducing mRNA and protein levels of bcl-2 and up regulating the p53 and caspase-3 mRNA and protein levels adversely affects the spermatogenesis and results in cellular depletion. Mashhad University of Medical Sciences 2017-02 /pmc/articles/PMC5339662/ /pubmed/28293398 http://dx.doi.org/10.22038/ijbms.2017.8249 Text en Copyright: © Iranian Journal of Basic Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Mosadegh, Maryam Hasanzadeh, Shapour Razi, Mazdak Nicotine-induced damages in testicular tissue of rats; evidences for bcl-2, p53 and caspase-3 expression |
title | Nicotine-induced damages in testicular tissue of rats; evidences for bcl-2, p53 and caspase-3 expression |
title_full | Nicotine-induced damages in testicular tissue of rats; evidences for bcl-2, p53 and caspase-3 expression |
title_fullStr | Nicotine-induced damages in testicular tissue of rats; evidences for bcl-2, p53 and caspase-3 expression |
title_full_unstemmed | Nicotine-induced damages in testicular tissue of rats; evidences for bcl-2, p53 and caspase-3 expression |
title_short | Nicotine-induced damages in testicular tissue of rats; evidences for bcl-2, p53 and caspase-3 expression |
title_sort | nicotine-induced damages in testicular tissue of rats; evidences for bcl-2, p53 and caspase-3 expression |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5339662/ https://www.ncbi.nlm.nih.gov/pubmed/28293398 http://dx.doi.org/10.22038/ijbms.2017.8249 |
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