Notch1 Signaling Contributes to Hypoxia-induced High Expression of Integrin β1 in Keratinocyte Migration

Oxygen tension is an important micro-environmental factor that affects epidermal development and function. After injury, high oxygen consumption and vascular injury result in partial hypoxia. However, whether hypoxia benefits or hurts wound healing remains controversial. In this study, a tissue oxyg...

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Autores principales: Tang, Di, Yan, Tiantian, Zhang, Junhui, Jiang, Xupin, Zhang, Dongxia, Huang, Yuesheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5339698/
https://www.ncbi.nlm.nih.gov/pubmed/28266574
http://dx.doi.org/10.1038/srep43926
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author Tang, Di
Yan, Tiantian
Zhang, Junhui
Jiang, Xupin
Zhang, Dongxia
Huang, Yuesheng
author_facet Tang, Di
Yan, Tiantian
Zhang, Junhui
Jiang, Xupin
Zhang, Dongxia
Huang, Yuesheng
author_sort Tang, Di
collection PubMed
description Oxygen tension is an important micro-environmental factor that affects epidermal development and function. After injury, high oxygen consumption and vascular injury result in partial hypoxia. However, whether hypoxia benefits or hurts wound healing remains controversial. In this study, a tissue oxygen tension monitor was used to detect the spatial and temporal distribution of oxygen in burn wounds. In vitro, we demonstrate that hypoxia promoted the expression of integrin β1 and the migration of keratinocytes. Furthermore, hypoxia-induced migration was slowed by Notch1 ligands and a siRNA against ITGB1 (integrin β1). Our findings suggest that integrin β1 may be an oxygen-sensitive molecule that promotes keratinocyte migration during wound healing and that Notch1 signaling is involved in this process.
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spelling pubmed-53396982017-03-10 Notch1 Signaling Contributes to Hypoxia-induced High Expression of Integrin β1 in Keratinocyte Migration Tang, Di Yan, Tiantian Zhang, Junhui Jiang, Xupin Zhang, Dongxia Huang, Yuesheng Sci Rep Article Oxygen tension is an important micro-environmental factor that affects epidermal development and function. After injury, high oxygen consumption and vascular injury result in partial hypoxia. However, whether hypoxia benefits or hurts wound healing remains controversial. In this study, a tissue oxygen tension monitor was used to detect the spatial and temporal distribution of oxygen in burn wounds. In vitro, we demonstrate that hypoxia promoted the expression of integrin β1 and the migration of keratinocytes. Furthermore, hypoxia-induced migration was slowed by Notch1 ligands and a siRNA against ITGB1 (integrin β1). Our findings suggest that integrin β1 may be an oxygen-sensitive molecule that promotes keratinocyte migration during wound healing and that Notch1 signaling is involved in this process. Nature Publishing Group 2017-03-07 /pmc/articles/PMC5339698/ /pubmed/28266574 http://dx.doi.org/10.1038/srep43926 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Tang, Di
Yan, Tiantian
Zhang, Junhui
Jiang, Xupin
Zhang, Dongxia
Huang, Yuesheng
Notch1 Signaling Contributes to Hypoxia-induced High Expression of Integrin β1 in Keratinocyte Migration
title Notch1 Signaling Contributes to Hypoxia-induced High Expression of Integrin β1 in Keratinocyte Migration
title_full Notch1 Signaling Contributes to Hypoxia-induced High Expression of Integrin β1 in Keratinocyte Migration
title_fullStr Notch1 Signaling Contributes to Hypoxia-induced High Expression of Integrin β1 in Keratinocyte Migration
title_full_unstemmed Notch1 Signaling Contributes to Hypoxia-induced High Expression of Integrin β1 in Keratinocyte Migration
title_short Notch1 Signaling Contributes to Hypoxia-induced High Expression of Integrin β1 in Keratinocyte Migration
title_sort notch1 signaling contributes to hypoxia-induced high expression of integrin β1 in keratinocyte migration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5339698/
https://www.ncbi.nlm.nih.gov/pubmed/28266574
http://dx.doi.org/10.1038/srep43926
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