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Confocal imaging of mouse mandibular condyle cartilage

Mice are commonly used to study the temporomandibular joint (TMJ) and to model human TMJ disease. However, evaluating TMJ pathology in mice using standard histologic methods is time consuming, labor intensive, and dependent upon investigators’ expertise at consistently orienting and sectioning acros...

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Autores principales: He, Y., Zhang, M., Huang, A. Y., Cui, Y., Bai, D., Warman, M. L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5339703/
https://www.ncbi.nlm.nih.gov/pubmed/28266618
http://dx.doi.org/10.1038/srep43848
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author He, Y.
Zhang, M.
Huang, A. Y.
Cui, Y.
Bai, D.
Warman, M. L.
author_facet He, Y.
Zhang, M.
Huang, A. Y.
Cui, Y.
Bai, D.
Warman, M. L.
author_sort He, Y.
collection PubMed
description Mice are commonly used to study the temporomandibular joint (TMJ) and to model human TMJ disease. However, evaluating TMJ pathology in mice using standard histologic methods is time consuming, labor intensive, and dependent upon investigators’ expertise at consistently orienting and sectioning across tiny specimens. We describe a method that uses confocal microscopy to rapidly and reliably assess indicators of mandibular condyle cartilage pathology in mice. We demonstrate the utility of this method for detecting abnormalities in chondrocyte distribution in mice lacking lubricin (Prg4), the major boundary lubricant of articular cartilage. We further show that the method can provide information about recombination sites and efficiency in mandibular cartilage for Cre-driver strains. Because specimen preparation and data acquisition with confocal microscopy are simple and fast, the method can serve as a primary screening tool for TMJ pathology, before proceeding to complicated, time consuming, secondary analyses.
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spelling pubmed-53397032017-03-10 Confocal imaging of mouse mandibular condyle cartilage He, Y. Zhang, M. Huang, A. Y. Cui, Y. Bai, D. Warman, M. L. Sci Rep Article Mice are commonly used to study the temporomandibular joint (TMJ) and to model human TMJ disease. However, evaluating TMJ pathology in mice using standard histologic methods is time consuming, labor intensive, and dependent upon investigators’ expertise at consistently orienting and sectioning across tiny specimens. We describe a method that uses confocal microscopy to rapidly and reliably assess indicators of mandibular condyle cartilage pathology in mice. We demonstrate the utility of this method for detecting abnormalities in chondrocyte distribution in mice lacking lubricin (Prg4), the major boundary lubricant of articular cartilage. We further show that the method can provide information about recombination sites and efficiency in mandibular cartilage for Cre-driver strains. Because specimen preparation and data acquisition with confocal microscopy are simple and fast, the method can serve as a primary screening tool for TMJ pathology, before proceeding to complicated, time consuming, secondary analyses. Nature Publishing Group 2017-03-07 /pmc/articles/PMC5339703/ /pubmed/28266618 http://dx.doi.org/10.1038/srep43848 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
He, Y.
Zhang, M.
Huang, A. Y.
Cui, Y.
Bai, D.
Warman, M. L.
Confocal imaging of mouse mandibular condyle cartilage
title Confocal imaging of mouse mandibular condyle cartilage
title_full Confocal imaging of mouse mandibular condyle cartilage
title_fullStr Confocal imaging of mouse mandibular condyle cartilage
title_full_unstemmed Confocal imaging of mouse mandibular condyle cartilage
title_short Confocal imaging of mouse mandibular condyle cartilage
title_sort confocal imaging of mouse mandibular condyle cartilage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5339703/
https://www.ncbi.nlm.nih.gov/pubmed/28266618
http://dx.doi.org/10.1038/srep43848
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