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Molecular outcomes, clinical consequences, and genetic diagnosis of Oculocutaneous Albinism in Pakistani population
Nonsyndromic oculocutaneous Albinism (nsOCA) is clinically characterized by the loss of pigmentation in the skin, hair, and iris. OCA is amongst the most common causes of vision impairment in children. To date, pathogenic variants in six genes have been identified in individuals with nsOCA. Here, we...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5339803/ https://www.ncbi.nlm.nih.gov/pubmed/28266639 http://dx.doi.org/10.1038/srep44185 |
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author | Shahzad, Mohsin Yousaf, Sairah Waryah, Yar M. Gul, Hadia Kausar, Tasleem Tariq, Nabeela Mahmood, Umair Ali, Muhammad Khan, Muzammil A. Waryah, Ali M. Shaikh, Rehan S. Riazuddin, Saima Ahmed, Zubair M. |
author_facet | Shahzad, Mohsin Yousaf, Sairah Waryah, Yar M. Gul, Hadia Kausar, Tasleem Tariq, Nabeela Mahmood, Umair Ali, Muhammad Khan, Muzammil A. Waryah, Ali M. Shaikh, Rehan S. Riazuddin, Saima Ahmed, Zubair M. |
author_sort | Shahzad, Mohsin |
collection | PubMed |
description | Nonsyndromic oculocutaneous Albinism (nsOCA) is clinically characterized by the loss of pigmentation in the skin, hair, and iris. OCA is amongst the most common causes of vision impairment in children. To date, pathogenic variants in six genes have been identified in individuals with nsOCA. Here, we determined the identities, frequencies, and clinical consequences of OCA alleles in 94 previously unreported Pakistani families. Combination of Sanger and Exome sequencing revealed 38 alleles, including 22 novel variants, segregating with nsOCA phenotype in 80 families. Variants of TYR and OCA2 genes were the most common cause of nsOCA, occurring in 43 and 30 families, respectively. Twenty-two novel variants include nine missense, four splice site, two non-sense, one insertion and six gross deletions. In vitro studies revealed retention of OCA proteins harboring novel missense alleles in the endoplasmic reticulum (ER) of transfected cells. Exon-trapping assays with constructs containing splice site alleles revealed errors in splicing. As eight alleles account for approximately 56% (95% CI: 46.52–65.24%) of nsOCA cases, primarily enrolled from Punjab province of Pakistan, hierarchical strategies for variant detection would be feasible and cost-efficient genetic tests for OCA in families with similar origin. Thus, we developed Tetra-primer ARMS assays for rapid, reliable, reproducible and economical screening of most of these common alleles. |
format | Online Article Text |
id | pubmed-5339803 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-53398032017-03-10 Molecular outcomes, clinical consequences, and genetic diagnosis of Oculocutaneous Albinism in Pakistani population Shahzad, Mohsin Yousaf, Sairah Waryah, Yar M. Gul, Hadia Kausar, Tasleem Tariq, Nabeela Mahmood, Umair Ali, Muhammad Khan, Muzammil A. Waryah, Ali M. Shaikh, Rehan S. Riazuddin, Saima Ahmed, Zubair M. Sci Rep Article Nonsyndromic oculocutaneous Albinism (nsOCA) is clinically characterized by the loss of pigmentation in the skin, hair, and iris. OCA is amongst the most common causes of vision impairment in children. To date, pathogenic variants in six genes have been identified in individuals with nsOCA. Here, we determined the identities, frequencies, and clinical consequences of OCA alleles in 94 previously unreported Pakistani families. Combination of Sanger and Exome sequencing revealed 38 alleles, including 22 novel variants, segregating with nsOCA phenotype in 80 families. Variants of TYR and OCA2 genes were the most common cause of nsOCA, occurring in 43 and 30 families, respectively. Twenty-two novel variants include nine missense, four splice site, two non-sense, one insertion and six gross deletions. In vitro studies revealed retention of OCA proteins harboring novel missense alleles in the endoplasmic reticulum (ER) of transfected cells. Exon-trapping assays with constructs containing splice site alleles revealed errors in splicing. As eight alleles account for approximately 56% (95% CI: 46.52–65.24%) of nsOCA cases, primarily enrolled from Punjab province of Pakistan, hierarchical strategies for variant detection would be feasible and cost-efficient genetic tests for OCA in families with similar origin. Thus, we developed Tetra-primer ARMS assays for rapid, reliable, reproducible and economical screening of most of these common alleles. Nature Publishing Group 2017-03-07 /pmc/articles/PMC5339803/ /pubmed/28266639 http://dx.doi.org/10.1038/srep44185 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Shahzad, Mohsin Yousaf, Sairah Waryah, Yar M. Gul, Hadia Kausar, Tasleem Tariq, Nabeela Mahmood, Umair Ali, Muhammad Khan, Muzammil A. Waryah, Ali M. Shaikh, Rehan S. Riazuddin, Saima Ahmed, Zubair M. Molecular outcomes, clinical consequences, and genetic diagnosis of Oculocutaneous Albinism in Pakistani population |
title | Molecular outcomes, clinical consequences, and genetic diagnosis of Oculocutaneous Albinism in Pakistani population |
title_full | Molecular outcomes, clinical consequences, and genetic diagnosis of Oculocutaneous Albinism in Pakistani population |
title_fullStr | Molecular outcomes, clinical consequences, and genetic diagnosis of Oculocutaneous Albinism in Pakistani population |
title_full_unstemmed | Molecular outcomes, clinical consequences, and genetic diagnosis of Oculocutaneous Albinism in Pakistani population |
title_short | Molecular outcomes, clinical consequences, and genetic diagnosis of Oculocutaneous Albinism in Pakistani population |
title_sort | molecular outcomes, clinical consequences, and genetic diagnosis of oculocutaneous albinism in pakistani population |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5339803/ https://www.ncbi.nlm.nih.gov/pubmed/28266639 http://dx.doi.org/10.1038/srep44185 |
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