Cargando…

Evaluation of two endometriosis models by transplantation of human endometrial tissue fragments and human endometrial mesenchymal cells

BACKGROUND: The animal models of endometriosis could be a valuable alternative tool for clarifying the etiology of endometriosis. OBJECTIVE: In this study two endometriosis models at the morphological and molecular levels was evaluated and compared. MATERIALS AND METHODS: The human endometrial tissu...

Descripción completa

Detalles Bibliográficos
Autores principales: Jafarabadi, Mina, Salehnia, Mojdeh, Sadafi, Rana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research and Clinical Center for Infertility 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5340136/
https://www.ncbi.nlm.nih.gov/pubmed/28280797
_version_ 1782512790437625856
author Jafarabadi, Mina
Salehnia, Mojdeh
Sadafi, Rana
author_facet Jafarabadi, Mina
Salehnia, Mojdeh
Sadafi, Rana
author_sort Jafarabadi, Mina
collection PubMed
description BACKGROUND: The animal models of endometriosis could be a valuable alternative tool for clarifying the etiology of endometriosis. OBJECTIVE: In this study two endometriosis models at the morphological and molecular levels was evaluated and compared. MATERIALS AND METHODS: The human endometrial tissues were cut into small fragments then they were randomly considered for transplantation into γ irradiated mice as model A; or they were isolated and cultured up to fourth passages. 2×10(6) cultured stromal cells were transplanted into γ irradiated mice subcutaneously as model B. twenty days later the ectopic tissues in both models were studied morphologically by Periodic acid-Schiff and hematoxylin and eosin staining. The expression of osteopontin (OPN) and matrix metalloproteinase 2 (MMP2) genes were also assessed using real time RT-PCR. 17-β estradiol levels of mice sera were compared before and after transplantation. RESULTS: The endometrial like glands and stromal cells were formed in the implanted subcutaneous tissue of both endometriosis models. The gland sections per cubic millimeter, the expression of OPN and MMP2 genes and the level of 17-β estradiol were higher in model B than model A (p=0.03). CONCLUSION: Our observation demonstrated that endometrial mesenchymal stromal cells showed more efficiency to establish endometriosis model than human endometrial tissue fragments.
format Online
Article
Text
id pubmed-5340136
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Research and Clinical Center for Infertility
record_format MEDLINE/PubMed
spelling pubmed-53401362017-03-09 Evaluation of two endometriosis models by transplantation of human endometrial tissue fragments and human endometrial mesenchymal cells Jafarabadi, Mina Salehnia, Mojdeh Sadafi, Rana Int J Reprod Biomed Original Article BACKGROUND: The animal models of endometriosis could be a valuable alternative tool for clarifying the etiology of endometriosis. OBJECTIVE: In this study two endometriosis models at the morphological and molecular levels was evaluated and compared. MATERIALS AND METHODS: The human endometrial tissues were cut into small fragments then they were randomly considered for transplantation into γ irradiated mice as model A; or they were isolated and cultured up to fourth passages. 2×10(6) cultured stromal cells were transplanted into γ irradiated mice subcutaneously as model B. twenty days later the ectopic tissues in both models were studied morphologically by Periodic acid-Schiff and hematoxylin and eosin staining. The expression of osteopontin (OPN) and matrix metalloproteinase 2 (MMP2) genes were also assessed using real time RT-PCR. 17-β estradiol levels of mice sera were compared before and after transplantation. RESULTS: The endometrial like glands and stromal cells were formed in the implanted subcutaneous tissue of both endometriosis models. The gland sections per cubic millimeter, the expression of OPN and MMP2 genes and the level of 17-β estradiol were higher in model B than model A (p=0.03). CONCLUSION: Our observation demonstrated that endometrial mesenchymal stromal cells showed more efficiency to establish endometriosis model than human endometrial tissue fragments. Research and Clinical Center for Infertility 2017-01 /pmc/articles/PMC5340136/ /pubmed/28280797 Text en http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Jafarabadi, Mina
Salehnia, Mojdeh
Sadafi, Rana
Evaluation of two endometriosis models by transplantation of human endometrial tissue fragments and human endometrial mesenchymal cells
title Evaluation of two endometriosis models by transplantation of human endometrial tissue fragments and human endometrial mesenchymal cells
title_full Evaluation of two endometriosis models by transplantation of human endometrial tissue fragments and human endometrial mesenchymal cells
title_fullStr Evaluation of two endometriosis models by transplantation of human endometrial tissue fragments and human endometrial mesenchymal cells
title_full_unstemmed Evaluation of two endometriosis models by transplantation of human endometrial tissue fragments and human endometrial mesenchymal cells
title_short Evaluation of two endometriosis models by transplantation of human endometrial tissue fragments and human endometrial mesenchymal cells
title_sort evaluation of two endometriosis models by transplantation of human endometrial tissue fragments and human endometrial mesenchymal cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5340136/
https://www.ncbi.nlm.nih.gov/pubmed/28280797
work_keys_str_mv AT jafarabadimina evaluationoftwoendometriosismodelsbytransplantationofhumanendometrialtissuefragmentsandhumanendometrialmesenchymalcells
AT salehniamojdeh evaluationoftwoendometriosismodelsbytransplantationofhumanendometrialtissuefragmentsandhumanendometrialmesenchymalcells
AT sadafirana evaluationoftwoendometriosismodelsbytransplantationofhumanendometrialtissuefragmentsandhumanendometrialmesenchymalcells