Molecular characterization of vancomycin-resistant Enterococcus faecium isolates from Bermuda

Molecular characteristics of vancomycin resistant enterococci isolates from Bermuda Island is currently unknown. This study was conducted to investigate phenotypic and genotypic characteristics of VRE isolates from Bermuda Island using the chromogenic agar, E-tests, polymerase chain reaction (PCR),...

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Autores principales: Akpaka, Patrick Eberechi, Kissoon, Shivnarine, Wilson, Clyde, Jayaratne, Padman, Smith, Ashley, Golding, George R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5340350/
https://www.ncbi.nlm.nih.gov/pubmed/28267763
http://dx.doi.org/10.1371/journal.pone.0171317
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author Akpaka, Patrick Eberechi
Kissoon, Shivnarine
Wilson, Clyde
Jayaratne, Padman
Smith, Ashley
Golding, George R.
author_facet Akpaka, Patrick Eberechi
Kissoon, Shivnarine
Wilson, Clyde
Jayaratne, Padman
Smith, Ashley
Golding, George R.
author_sort Akpaka, Patrick Eberechi
collection PubMed
description Molecular characteristics of vancomycin resistant enterococci isolates from Bermuda Island is currently unknown. This study was conducted to investigate phenotypic and genotypic characteristics of VRE isolates from Bermuda Island using the chromogenic agar, E-tests, polymerase chain reaction (PCR), pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Eighteen E. faecium isolates were completely analyzed and were all resistant to vancomycin, susceptible to linezolid and quinupristin/dalfopristin, positive for vanA and esp genes. The MLST analysis confirmed most isolates were of the sequence types linked to clonal complex 17 (CC17) that is widely associated with outbreaks in hospitals. Infection control measures, antibiotic stewardship, and surveillance activities will continue to be a priority in hospital on the Island.
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spelling pubmed-53403502017-03-10 Molecular characterization of vancomycin-resistant Enterococcus faecium isolates from Bermuda Akpaka, Patrick Eberechi Kissoon, Shivnarine Wilson, Clyde Jayaratne, Padman Smith, Ashley Golding, George R. PLoS One Research Article Molecular characteristics of vancomycin resistant enterococci isolates from Bermuda Island is currently unknown. This study was conducted to investigate phenotypic and genotypic characteristics of VRE isolates from Bermuda Island using the chromogenic agar, E-tests, polymerase chain reaction (PCR), pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Eighteen E. faecium isolates were completely analyzed and were all resistant to vancomycin, susceptible to linezolid and quinupristin/dalfopristin, positive for vanA and esp genes. The MLST analysis confirmed most isolates were of the sequence types linked to clonal complex 17 (CC17) that is widely associated with outbreaks in hospitals. Infection control measures, antibiotic stewardship, and surveillance activities will continue to be a priority in hospital on the Island. Public Library of Science 2017-03-07 /pmc/articles/PMC5340350/ /pubmed/28267763 http://dx.doi.org/10.1371/journal.pone.0171317 Text en © 2017 Akpaka et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Akpaka, Patrick Eberechi
Kissoon, Shivnarine
Wilson, Clyde
Jayaratne, Padman
Smith, Ashley
Golding, George R.
Molecular characterization of vancomycin-resistant Enterococcus faecium isolates from Bermuda
title Molecular characterization of vancomycin-resistant Enterococcus faecium isolates from Bermuda
title_full Molecular characterization of vancomycin-resistant Enterococcus faecium isolates from Bermuda
title_fullStr Molecular characterization of vancomycin-resistant Enterococcus faecium isolates from Bermuda
title_full_unstemmed Molecular characterization of vancomycin-resistant Enterococcus faecium isolates from Bermuda
title_short Molecular characterization of vancomycin-resistant Enterococcus faecium isolates from Bermuda
title_sort molecular characterization of vancomycin-resistant enterococcus faecium isolates from bermuda
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5340350/
https://www.ncbi.nlm.nih.gov/pubmed/28267763
http://dx.doi.org/10.1371/journal.pone.0171317
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