Cargando…
DNA methylation and gene expression changes derived from assisted reproductive technologies can be decreased by reproductive fluids
The number of children born since the origin of Assisted Reproductive Technologies (ART) exceeds 5 million. The majority seem healthy, but a higher frequency of defects has been reported among ART-conceived infants, suggesting an epigenetic cost. We report the first whole-genome DNA methylation data...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5340525/ https://www.ncbi.nlm.nih.gov/pubmed/28134613 http://dx.doi.org/10.7554/eLife.23670 |
_version_ | 1782512845603209216 |
---|---|
author | Canovas, Sebastian Ivanova, Elena Romar, Raquel García-Martínez, Soledad Soriano-Úbeda, Cristina García-Vázquez, Francisco A Saadeh, Heba Andrews, Simon Kelsey, Gavin Coy, Pilar |
author_facet | Canovas, Sebastian Ivanova, Elena Romar, Raquel García-Martínez, Soledad Soriano-Úbeda, Cristina García-Vázquez, Francisco A Saadeh, Heba Andrews, Simon Kelsey, Gavin Coy, Pilar |
author_sort | Canovas, Sebastian |
collection | PubMed |
description | The number of children born since the origin of Assisted Reproductive Technologies (ART) exceeds 5 million. The majority seem healthy, but a higher frequency of defects has been reported among ART-conceived infants, suggesting an epigenetic cost. We report the first whole-genome DNA methylation datasets from single pig blastocysts showing differences between in vivo and in vitro produced embryos. Blastocysts were produced in vitro either without (C-IVF) or in the presence of natural reproductive fluids (Natur-IVF). Natur-IVF embryos were of higher quality than C-IVF in terms of cell number and hatching ability. RNA-Seq and DNA methylation analyses showed that Natur-IVF embryos have expression and methylation patterns closer to in vivo blastocysts. Genes involved in reprogramming, imprinting and development were affected by culture, with fewer aberrations in Natur-IVF embryos. Methylation analysis detected methylated changes in C-IVF, but not in Natur-IVF, at genes whose methylation could be critical, such as IGF2R and NNAT. DOI: http://dx.doi.org/10.7554/eLife.23670.001 |
format | Online Article Text |
id | pubmed-5340525 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-53405252017-03-10 DNA methylation and gene expression changes derived from assisted reproductive technologies can be decreased by reproductive fluids Canovas, Sebastian Ivanova, Elena Romar, Raquel García-Martínez, Soledad Soriano-Úbeda, Cristina García-Vázquez, Francisco A Saadeh, Heba Andrews, Simon Kelsey, Gavin Coy, Pilar eLife Developmental Biology and Stem Cells The number of children born since the origin of Assisted Reproductive Technologies (ART) exceeds 5 million. The majority seem healthy, but a higher frequency of defects has been reported among ART-conceived infants, suggesting an epigenetic cost. We report the first whole-genome DNA methylation datasets from single pig blastocysts showing differences between in vivo and in vitro produced embryos. Blastocysts were produced in vitro either without (C-IVF) or in the presence of natural reproductive fluids (Natur-IVF). Natur-IVF embryos were of higher quality than C-IVF in terms of cell number and hatching ability. RNA-Seq and DNA methylation analyses showed that Natur-IVF embryos have expression and methylation patterns closer to in vivo blastocysts. Genes involved in reprogramming, imprinting and development were affected by culture, with fewer aberrations in Natur-IVF embryos. Methylation analysis detected methylated changes in C-IVF, but not in Natur-IVF, at genes whose methylation could be critical, such as IGF2R and NNAT. DOI: http://dx.doi.org/10.7554/eLife.23670.001 eLife Sciences Publications, Ltd 2017-02-01 /pmc/articles/PMC5340525/ /pubmed/28134613 http://dx.doi.org/10.7554/eLife.23670 Text en © 2017, Canovas et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Developmental Biology and Stem Cells Canovas, Sebastian Ivanova, Elena Romar, Raquel García-Martínez, Soledad Soriano-Úbeda, Cristina García-Vázquez, Francisco A Saadeh, Heba Andrews, Simon Kelsey, Gavin Coy, Pilar DNA methylation and gene expression changes derived from assisted reproductive technologies can be decreased by reproductive fluids |
title | DNA methylation and gene expression changes derived from assisted reproductive technologies can be decreased by reproductive fluids |
title_full | DNA methylation and gene expression changes derived from assisted reproductive technologies can be decreased by reproductive fluids |
title_fullStr | DNA methylation and gene expression changes derived from assisted reproductive technologies can be decreased by reproductive fluids |
title_full_unstemmed | DNA methylation and gene expression changes derived from assisted reproductive technologies can be decreased by reproductive fluids |
title_short | DNA methylation and gene expression changes derived from assisted reproductive technologies can be decreased by reproductive fluids |
title_sort | dna methylation and gene expression changes derived from assisted reproductive technologies can be decreased by reproductive fluids |
topic | Developmental Biology and Stem Cells |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5340525/ https://www.ncbi.nlm.nih.gov/pubmed/28134613 http://dx.doi.org/10.7554/eLife.23670 |
work_keys_str_mv | AT canovassebastian dnamethylationandgeneexpressionchangesderivedfromassistedreproductivetechnologiescanbedecreasedbyreproductivefluids AT ivanovaelena dnamethylationandgeneexpressionchangesderivedfromassistedreproductivetechnologiescanbedecreasedbyreproductivefluids AT romarraquel dnamethylationandgeneexpressionchangesderivedfromassistedreproductivetechnologiescanbedecreasedbyreproductivefluids AT garciamartinezsoledad dnamethylationandgeneexpressionchangesderivedfromassistedreproductivetechnologiescanbedecreasedbyreproductivefluids AT sorianoubedacristina dnamethylationandgeneexpressionchangesderivedfromassistedreproductivetechnologiescanbedecreasedbyreproductivefluids AT garciavazquezfranciscoa dnamethylationandgeneexpressionchangesderivedfromassistedreproductivetechnologiescanbedecreasedbyreproductivefluids AT saadehheba dnamethylationandgeneexpressionchangesderivedfromassistedreproductivetechnologiescanbedecreasedbyreproductivefluids AT andrewssimon dnamethylationandgeneexpressionchangesderivedfromassistedreproductivetechnologiescanbedecreasedbyreproductivefluids AT kelseygavin dnamethylationandgeneexpressionchangesderivedfromassistedreproductivetechnologiescanbedecreasedbyreproductivefluids AT coypilar dnamethylationandgeneexpressionchangesderivedfromassistedreproductivetechnologiescanbedecreasedbyreproductivefluids |