Cargando…

Duodenal Endoluminal Barrier Sleeve Alters Gut Microbiota of ZDF rats

BACKGROUND/OBJECTIVES: The combination of energy dense diets and reduced energy expenditure in modern society has escalated the prevalence of obesity and obesity-related comorbidities. Among these disease-states, type-2 diabetics (T2D) are disproportionately associated with obesity, suggesting a sha...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Teayoun, Holleman, Cassie L, Ptacek, Travis, Morrow, Casey D, Habegger, Kirk M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5340580/
https://www.ncbi.nlm.nih.gov/pubmed/27924082
http://dx.doi.org/10.1038/ijo.2016.224
_version_ 1782512851429097472
author Kim, Teayoun
Holleman, Cassie L
Ptacek, Travis
Morrow, Casey D
Habegger, Kirk M
author_facet Kim, Teayoun
Holleman, Cassie L
Ptacek, Travis
Morrow, Casey D
Habegger, Kirk M
author_sort Kim, Teayoun
collection PubMed
description BACKGROUND/OBJECTIVES: The combination of energy dense diets and reduced energy expenditure in modern society has escalated the prevalence of obesity and obesity-related comorbidities. Among these disease-states, type-2 diabetics (T2D) are disproportionately associated with obesity, suggesting a shared etiology. In conjunction with defects in hormonal and inflammatory states, obesity and T2D are also characterized by dysbiosis. METHODS: We have recently described the beneficial effects of duodenal nutrient exclusion, as induced by the duodenal endoluminal sleeve (DES); including body weight loss, prevented fat mass accumulation, and improved glucose tolerance in the ZDF rat, a rodent model of obesity and type-2 diabetes (T2D). To assess the relative role of DES on hindgut microbiota in the context of these metabolic changes, we analyzed cecal samples from rats implanted with a duodenal endoluminal sleeve (DES), or a sham control of this procedure. A group of pair-fed (pf) sham controls was also included to account for changes induced by reduced body weight and food intake. RESULTS: Analysis of hind gut microbiota following DES in the ZDF rat elucidated discrete changes in several microbial populations including a reduction in Paraprevotella family members of the Clostridiales order along with an increase in Akkermansia muciniphila and species of the Allobaculum and Bifidobacterium genera. CONCLUSIONS: Together these observations suggest that like Roux-en Y gastric bypass (RYGB) and Metformin, regulation of gut microbiota may be a contributing factor to the therapeutic effects of DES.
format Online
Article
Text
id pubmed-5340580
institution National Center for Biotechnology Information
language English
publishDate 2016
record_format MEDLINE/PubMed
spelling pubmed-53405802017-06-07 Duodenal Endoluminal Barrier Sleeve Alters Gut Microbiota of ZDF rats Kim, Teayoun Holleman, Cassie L Ptacek, Travis Morrow, Casey D Habegger, Kirk M Int J Obes (Lond) Article BACKGROUND/OBJECTIVES: The combination of energy dense diets and reduced energy expenditure in modern society has escalated the prevalence of obesity and obesity-related comorbidities. Among these disease-states, type-2 diabetics (T2D) are disproportionately associated with obesity, suggesting a shared etiology. In conjunction with defects in hormonal and inflammatory states, obesity and T2D are also characterized by dysbiosis. METHODS: We have recently described the beneficial effects of duodenal nutrient exclusion, as induced by the duodenal endoluminal sleeve (DES); including body weight loss, prevented fat mass accumulation, and improved glucose tolerance in the ZDF rat, a rodent model of obesity and type-2 diabetes (T2D). To assess the relative role of DES on hindgut microbiota in the context of these metabolic changes, we analyzed cecal samples from rats implanted with a duodenal endoluminal sleeve (DES), or a sham control of this procedure. A group of pair-fed (pf) sham controls was also included to account for changes induced by reduced body weight and food intake. RESULTS: Analysis of hind gut microbiota following DES in the ZDF rat elucidated discrete changes in several microbial populations including a reduction in Paraprevotella family members of the Clostridiales order along with an increase in Akkermansia muciniphila and species of the Allobaculum and Bifidobacterium genera. CONCLUSIONS: Together these observations suggest that like Roux-en Y gastric bypass (RYGB) and Metformin, regulation of gut microbiota may be a contributing factor to the therapeutic effects of DES. 2016-12-07 2017-03 /pmc/articles/PMC5340580/ /pubmed/27924082 http://dx.doi.org/10.1038/ijo.2016.224 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Kim, Teayoun
Holleman, Cassie L
Ptacek, Travis
Morrow, Casey D
Habegger, Kirk M
Duodenal Endoluminal Barrier Sleeve Alters Gut Microbiota of ZDF rats
title Duodenal Endoluminal Barrier Sleeve Alters Gut Microbiota of ZDF rats
title_full Duodenal Endoluminal Barrier Sleeve Alters Gut Microbiota of ZDF rats
title_fullStr Duodenal Endoluminal Barrier Sleeve Alters Gut Microbiota of ZDF rats
title_full_unstemmed Duodenal Endoluminal Barrier Sleeve Alters Gut Microbiota of ZDF rats
title_short Duodenal Endoluminal Barrier Sleeve Alters Gut Microbiota of ZDF rats
title_sort duodenal endoluminal barrier sleeve alters gut microbiota of zdf rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5340580/
https://www.ncbi.nlm.nih.gov/pubmed/27924082
http://dx.doi.org/10.1038/ijo.2016.224
work_keys_str_mv AT kimteayoun duodenalendoluminalbarriersleevealtersgutmicrobiotaofzdfrats
AT hollemancassiel duodenalendoluminalbarriersleevealtersgutmicrobiotaofzdfrats
AT ptacektravis duodenalendoluminalbarriersleevealtersgutmicrobiotaofzdfrats
AT morrowcaseyd duodenalendoluminalbarriersleevealtersgutmicrobiotaofzdfrats
AT habeggerkirkm duodenalendoluminalbarriersleevealtersgutmicrobiotaofzdfrats