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Alteration of T cell immunity by lentiviral transduction of human monocyte-derived dendritic cells
BACKGROUND: Dendritic cells (DCs) are professional antigen-presenting cells that play important roles during human immunodeficiency virus type 1 (HIV-1) infection. HIV-1 derived lentiviral vectors (LVs) transduce DCs at high efficiency but their effects on DC functions have not been carefully studie...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2004
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC534092/ https://www.ncbi.nlm.nih.gov/pubmed/15518595 http://dx.doi.org/10.1186/1742-4690-1-37 |
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author | Chen, Xiaochuan He, Jin Chang, Lung-Ji |
author_facet | Chen, Xiaochuan He, Jin Chang, Lung-Ji |
author_sort | Chen, Xiaochuan |
collection | PubMed |
description | BACKGROUND: Dendritic cells (DCs) are professional antigen-presenting cells that play important roles during human immunodeficiency virus type 1 (HIV-1) infection. HIV-1 derived lentiviral vectors (LVs) transduce DCs at high efficiency but their effects on DC functions have not been carefully studied. Modification of DCs using LVs may lead to important applications in transplantation, treatment of cancer, autoimmune and infectious diseases. RESULTS: Using DCs prepared from multiple blood donors, we report that LV transduction of DCs resulted in altered DC phenotypes and functions. Lentiviral transduction of DCs resulted in down-regulation of cell surface molecules including CD1a, co-stimulatory molecules CD80, CD86, ICAM-1, and DC-SIGN. DCs transduced with LVs displayed a diminished capacity to polarize naive T cells to differentiate into Th1 effectors. This impaired Th1 response could be fully corrected by co-transduction of DCs with LVs encoding interleukin-12 (IL-12), interferon-gamma (IFN-γ), or small interfering RNA (siRNA) targeting IL-10. CONCLUSIONS: DCs transduced with LVs in vitro displayed diminished Th1 functions due to altered DC phenotypes. Our study addresses an important issue concerning lentiviral infection and modification of DC functions, and provides a rational approach using LVs for immunotherapy. |
format | Text |
id | pubmed-534092 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2004 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-5340922004-11-28 Alteration of T cell immunity by lentiviral transduction of human monocyte-derived dendritic cells Chen, Xiaochuan He, Jin Chang, Lung-Ji Retrovirology Research BACKGROUND: Dendritic cells (DCs) are professional antigen-presenting cells that play important roles during human immunodeficiency virus type 1 (HIV-1) infection. HIV-1 derived lentiviral vectors (LVs) transduce DCs at high efficiency but their effects on DC functions have not been carefully studied. Modification of DCs using LVs may lead to important applications in transplantation, treatment of cancer, autoimmune and infectious diseases. RESULTS: Using DCs prepared from multiple blood donors, we report that LV transduction of DCs resulted in altered DC phenotypes and functions. Lentiviral transduction of DCs resulted in down-regulation of cell surface molecules including CD1a, co-stimulatory molecules CD80, CD86, ICAM-1, and DC-SIGN. DCs transduced with LVs displayed a diminished capacity to polarize naive T cells to differentiate into Th1 effectors. This impaired Th1 response could be fully corrected by co-transduction of DCs with LVs encoding interleukin-12 (IL-12), interferon-gamma (IFN-γ), or small interfering RNA (siRNA) targeting IL-10. CONCLUSIONS: DCs transduced with LVs in vitro displayed diminished Th1 functions due to altered DC phenotypes. Our study addresses an important issue concerning lentiviral infection and modification of DC functions, and provides a rational approach using LVs for immunotherapy. BioMed Central 2004-11-01 /pmc/articles/PMC534092/ /pubmed/15518595 http://dx.doi.org/10.1186/1742-4690-1-37 Text en Copyright © 2004 Chen et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Chen, Xiaochuan He, Jin Chang, Lung-Ji Alteration of T cell immunity by lentiviral transduction of human monocyte-derived dendritic cells |
title | Alteration of T cell immunity by lentiviral transduction of human monocyte-derived dendritic cells |
title_full | Alteration of T cell immunity by lentiviral transduction of human monocyte-derived dendritic cells |
title_fullStr | Alteration of T cell immunity by lentiviral transduction of human monocyte-derived dendritic cells |
title_full_unstemmed | Alteration of T cell immunity by lentiviral transduction of human monocyte-derived dendritic cells |
title_short | Alteration of T cell immunity by lentiviral transduction of human monocyte-derived dendritic cells |
title_sort | alteration of t cell immunity by lentiviral transduction of human monocyte-derived dendritic cells |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC534092/ https://www.ncbi.nlm.nih.gov/pubmed/15518595 http://dx.doi.org/10.1186/1742-4690-1-37 |
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